Effects of karanjin on cell cycle arrest and apoptosis in human A549, HepG2 and HL-60 cancer cells

  • Biol Res. 2015 Jul 26;48(1):40. doi: 10.1186/s40659-015-0031-x.
Jian-Ru Guo  1 ,  Qian-Qian Chen  2 ,  Christopher Wai-Kei Lam  3 ,  Wei Zhang  4
Affiliations
  • 1. State Key Laboratory of Quality Research in Chinese Medicines, Macau Institute for Applied Research in Medicine and Health, Macau University of Science and Technology, Taipa, Macau, China. [email protected].
  • 2. State Key Laboratory of Quality Research in Chinese Medicines, Macau Institute for Applied Research in Medicine and Health, Macau University of Science and Technology, Taipa, Macau, China. [email protected].
  • 3. State Key Laboratory of Quality Research in Chinese Medicines, Macau Institute for Applied Research in Medicine and Health, Macau University of Science and Technology, Taipa, Macau, China. [email protected].
  • 4. State Key Laboratory of Quality Research in Chinese Medicines, Macau Institute for Applied Research in Medicine and Health, Macau University of Science and Technology, Taipa, Macau, China. [email protected].
PMID: 26209237 DOI: 10.1186/s40659-015-0031-x
Abstract

Background: We have investigated the potential Anticancer effects of karanjin, a principal furanoflavonol constituent of the Chinese medicine Fordia cauliflora, using cytotoxic assay, cell cycle arrest, and induction of Apoptosis in three human Cancer cell lines (A549, HepG2 and HL-60 cells).

Results: MTT cytotoxic assay showed that karanjin could inhibit the proliferation and viability of all three Cancer cells. The induction of cell cycle arrest was observed via a PI (propidium iodide)/RNase Staining Buffer detection kit and analyzed by flow cytometry: karanjin could dose-dependently induce cell cycle arrest at G2/M phase in the three cell lines. Cell Apoptosis was assessed by Annexin V-FITC/PI staining: all three Cancer cells treated with karanjin exhibited significantly increased apoptotic rates, especially in the percentage of late Apoptosis cells.

Conclusion: Karanjin can induce Cancer cell death through cell cycle arrest and enhance Apoptosis. This compound may be effective clinically for Cancer pharmacotherapy.

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