2. Epigenetics PI3K/Akt/mTOR Apoptosis Metabolic Enzyme/Protease NF-κB
  3. AMPK Apoptosis Cytochrome P450 Phosphatase NF-κB
  4. Karanjin

Karanjin is an orally active furanoflavonoid which can be isolated from several Leguminosae. Karanjin exhibits evident anti-diabetic, anti-cancer, anti-inflammatory, antioxidant, anticolitis, anti-ulcer, anti-Alzheimer properties and multiple insect repellent/insecticidal, acaricide properties, suggesting the potential of Karanjin to be applied to relevant research.

For research use only. We do not sell to patients.

Karanjin Chemical Structure

Karanjin Chemical Structure

CAS No. : 521-88-0

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10 mg USD 210 In-stock
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Based on 1 publication(s) in Google Scholar

Karanjin Related Antibodies

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  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

Karanjin is an orally active furanoflavonoid which can be isolated from several Leguminosae. Karanjin exhibits evident anti-diabetic, anti-cancer, anti-inflammatory, antioxidant, anticolitis, anti-ulcer, anti-Alzheimer properties and multiple insect repellent/insecticidal, acaricide properties, suggesting the potential of Karanjin to be applied to relevant research[1].

In Vitro

Karanjin (10-25 μM, 16 h) enhances glucose uptake in L6-GLUT4 myc cells through stimulates translocation of GLUT4 to plasma membrane-associated with activation of AMPK pathway and inhibits the activity of PTPase in a dose-dependent manner. Shows no significant effect on cell viability[1].
Karanjin (4-8 μM, 72 h) blocks the cell cycle of A549 and HL-60 markedly as well as induces significant cell apoptosis[1].
Karanjin (80, 160 μM, 30 min) decreases ROS level by inhibition of I-κB degradation resulting in restriction of NF-κB nuclear translocation in Hela cells[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Karanjin Related Antibodies

Cell Cycle Analysis[1]

Cell Line: A549, HL-60
Concentration: 4, 6, 8 μM for A549, 2, 4, 6 μM for HL-60
Incubation Time: 72 h
Result: Blocked the cell cycle markedly at G2/M phase of A549 (>60% for 8 μM) and HL-60 (>40% for 6 μM)
In Vivo

Karanjin (50, 100 mg/kg, p.o., 6 h) reduces the blood glucose level of Streptozotocin (HY-13753)-induced diabetic rats[1].
Karanjin (20 mg/kg/d, p.o., 21 d) reduces joint injury and cartilage damage along with edema and erythema in the AIA model rats[1].
Karanjin (10, 20 mg/kg/d, p.o., 14 d) significantly decreases serum ACP, ALP and TNFα levels in the AIA model rats [1].
Karanjin (25 or 50 mg/kg/d, p.o. or i.g., 8 d) improved learning and memory in Diazepam-induced amnesia mice[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Streptozotocin-induced diabetic rats[1]
Dosage: 50, 100 mg/kg
Administration: Oral gavage (p.o.), wait for 6h
Result: Lowered the blood glucose level by 11.7% and 20.7% at 50 and 100 mg/kg gavage.
Animal Model: Adjuvant induced arthritis model (AIA) Wistar strain male albino rats[1]
Dosage: 10, 20 mg/kg/d
Administration: Oral gavage (p.o.), 14 d for serum assay, 21 d for histological examination
Result: Reduced articular cartilage damage, cellular infiltration in the articular cartilage and spongy bone damage significantly. Decreased serum acid phosphatase, alkaline phosphatase levels and TNFα level markedly.
Animal Model: Diazepam-induced male swiss albino mice [1]
Dosage: 25, 50 mg/kg/d
Administration: Oral gavage (p.o.) or Intraperitoneal injection (i.p.) for 8 d
Result: Decreased the transfer latency on all the observation days, significantly reversed diazepam-induced amnesia, indicating improved learning and memory in treated mice.
Molecular Weight

292.29

Formula

C18H12O4
CAS No.
Appearance

Solid

Color

White to off-white

SMILES

O=C1C2=CC=C(OC=C3)C3=C2OC(C4=CC=CC=C4)=C1OC
Structure Classification
Initial Source
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, sealed storage, away from moisture and light

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

Solvent & Solubility
In Vitro: 

DMSO : 25 mg/mL (85.53 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 3.4213 mL 17.1063 mL 34.2126 mL
5 mM 0.6843 mL 3.4213 mL 6.8425 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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Concentration
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Volume
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Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

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C2

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In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    90% Corn Oil

    Solubility: ≥ 2.5 mg/mL (8.55 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
% 
+
%
Tween-80 +
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation

Purity: 99.75%

COA (256 KB) HNMR (245 KB) LCMS (93 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 3.4213 mL 17.1063 mL 34.2126 mL 85.5315 mL
5 mM 0.6843 mL 3.4213 mL 6.8425 mL 17.1063 mL
10 mM 0.3421 mL 1.7106 mL 3.4213 mL 8.5531 mL
15 mM 0.2281 mL 1.1404 mL 2.2808 mL 5.7021 mL
20 mM 0.1711 mL 0.8553 mL 1.7106 mL 4.2766 mL
25 mM 0.1369 mL 0.6843 mL 1.3685 mL 3.4213 mL
30 mM 0.1140 mL 0.5702 mL 1.1404 mL 2.8510 mL
40 mM 0.0855 mL 0.4277 mL 0.8553 mL 2.1383 mL
50 mM 0.0684 mL 0.3421 mL 0.6843 mL 1.7106 mL
60 mM 0.0570 mL 0.2851 mL 0.5702 mL 1.4255 mL
80 mM 0.0428 mL 0.2138 mL 0.4277 mL 1.0691 mL
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Karanjin Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Karanjin521-88-0AMPKApoptosisCytochrome P450PhosphataseNF-κBAMP-activated protein kinaseCYPsNuclear factor-κBNuclear factor-kappaBapoptosisanti-diabeticanti-canceranti-inflammatoryanti-hyperglycemicantioxidantanticolitisanti-ulceranti-AlzheimerL6-GLUT4 myc cellsA549HL-60HelaInhibitorinhibitorinhibit

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