Effects of activating GABAB1 receptor on proliferation, migration, invasion and epithelial-mesenchymal transition of ovarian cancer cells

  • J Ovarian Res. 2020 Oct 24;13(1):126. doi: 10.1186/s13048-020-00726-4.
Jun Gao  1 ,  Yao Gao  2 ,  Shixin Lin  2 ,  Xia Zou  1 ,  Yukai Zhu  2 ,  Xintong Chen  2 ,  Hong Wan  3 ,  Hong Zhu  4
Affiliations
  • 1. Department of Obstetrics and Gynecology, the Third Affiliated Hospital of Nanchang University, Xiangshanbei Road 128, Donghu District, Nanchang, 330008, Jiangxi, China.
  • 2. Postgraduate Department, Jiangxi Medical College of Nanchang University, Nanchang, 330008, Jiangxi, China.
  • 3. Department of Obstetrics and Gynecology, the Third Affiliated Hospital of Nanchang University, Xiangshanbei Road 128, Donghu District, Nanchang, 330008, Jiangxi, China. [email protected].
  • 4. Department of Obstetrics and Gynecology, the Third Affiliated Hospital of Nanchang University, Xiangshanbei Road 128, Donghu District, Nanchang, 330008, Jiangxi, China. [email protected].
PMID: 33099319 DOI: 10.1186/s13048-020-00726-4
Abstract

Objective: This study aimed to explore the effects of activating GABAB1 receptor by baclofen on proliferation, migration, invasion and epithelial-mesenchymal transition (EMT) of ovarian Cancer cells.

Results: One hundred μmol/L, 200 μmol/L and 300 μmol/L were selected as low, medium and high baclofen concentrations respectively. Cells were divided into four groups: Control, 100 μmol/L, 200 μmol/L and 300 μmol/L. Compared with the control group, the viability, colony formation, migration and invasion of SKOV3 cells were inhibited, and the Apoptosis of SKOV3 cells were enhanced significantly at 200 μmol/L and 300 μmol/L baclofen. Moreover, they changed significantly with the increase of baclofen concentration. Compared with the control group, the expression of E-cadherin and GABAB1 increased and the N-Cadherin expression decreased significantly in 200 μmol/L and 300 μmol/L groups. Higher concentration of baclofen induced higher expression of E-cadherin and lower expression of N-Cadherin.

Conclusion: Baclofen inhibited the proliferation, cloning, migration, invasion and EMT of ovarian Cancer cells by activating GABAB1 receptor. These results might contribute a lot to clarify the role and possible mechanism of GABAB1 receptor in ovarian Cancer.

Keywords
Baclofen; Epithelial-mesenchymal transition; G protein-coupled receptor; GABAB1 receptor; Ovarian cancer.
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