Parathyroid hormone-related protein (1-37) induces cAMP response in human osteoblast-like cells

  • Calcif Tissue Int. 1998 Mar;62(3):250-4. doi: 10.1007/s002239900425.
J A Tsai  1 ,  E Bucht ,  A Stark ,  U Sjöstedt ,  O Törring
Affiliations
  • 1. Department of Molecular Medicine, Karolinska Hospital and Institute, Stockholm, Sweden.
PMID: 9501959 DOI: 10.1007/s002239900425
Abstract

During recent years parathyroid hormone-related protein (PTHrP) research has been focused on the physiological functions of different fragments of the PTHrP molecule. Here we demonstrate that PTHrP (1-37) induced cyclic adenosine monophosphate (cAMP) response in primary human osteoblast-like cells, which were well characterized by the presence of Alkaline Phosphatase activity and osteocalcin production after stimulation with 1,25-dihydroxyvitamin D3. However, there was no cAMP response to PTHrP (58-77). Furthermore, the response to PTHrP (1-37) was dose dependent, with a significant increase at 1 nM. The presence of PTHrP (1-37)-induced cAMP response in human osteoblast-like cells implies that aminoterminal PTHrP fragments may exert important functions in the bone.

Products