2. Anti-infection
  3. SARS-CoV HBV
  4. Punicalagin

Punicalagin is a polyphenol ingredient isolated from Pomegranate (Punica granatum L.) or the leaves of Terminalia catappa L.. Punicalagin is a reversible and non-competitive 3CLpro inhibitor and inhibits SARS-CoV-2 replication in vitro. Punicalagin is an anti-hepatitis B virus (HBV) agent and has antioxidant, anti-inflammatory, and anticancer effects. Punicalagin has the potential for the research of COVID-19.

For research use only. We do not sell to patients.

CAS No. : 65995-63-3

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Based on 15 publication(s) in Google Scholar

Other Forms of Punicalagin:

Punicalagin Related Antibodies

Top Publications Citing Use of Products

    Punicalagin purchased from MedChemExpress. Usage Cited in: Acta Pharmacol Sin. 2025 Sep 3.  [Abstract]

    Body weight changes in Female BALB/c mice aged 6–8 weeks after treatment with 20 mg/kg of Salvianolic acid A (SAA), Chebulinic Acid, Tannic acid, or Punicalagin via i.g. upon challenge once daily for 3 days. 300 mg/kg T-705 administered via i.g. was used as a positive control.

    Punicalagin purchased from MedChemExpress. Usage Cited in: Acta Pharmacol Sin. 2025 Sep 3.  [Abstract]

    T-705 and SAA significantly reduced viral copies in the liver in Female BALB/c mice aged 6–8 weeks after treatment with 20 mg/kg of Salvianolic acid A (SAA), Chebulinic Acid, Tannic acid, or Punicalagin via i.g. upon challenge once daily for 3 days. 300 mg/kg T-705 administered via i.g. was used as a positive control.

    Punicalagin purchased from MedChemExpress. Usage Cited in: Phytomedicine. 2025 Jan 1:136:156330.  [Abstract]

    Punicalagin (PUN)-treated HIE group was administered various doses of PUN (10 and 20 mg/kg) 24 h before hypoxia by intraperitoneal (i.p.) injection at 24 h intervals in Sprague-Dawley (SD) rats. The mRNA expression of the inflammatory factors Ccl6, Lgals3, C1qa, TYROBP, and CD74 in the brain tissues 48 h postoperatively, normalized to GAPDH for each sample. PUN can effectively reduce the inflammatory response in brain tissue after HIE, while the AKT signaling pathway inhibitor 3-MA reversed this therapeutic effect.

    Punicalagin purchased from MedChemExpress. Usage Cited in: Phytomedicine. 2025 Jan 1:136:156330.  [Abstract]

    Punicalagin (PUN)-treated HIE group was administered various doses of PUN (10 and 20 mg/kg) 24 h before hypoxia by intraperitoneal (i.p.) injection at 24 h intervals in Sprague-Dawley (SD) rats. Representative images of HE staining in the cortex post-operatively.

    Punicalagin purchased from MedChemExpress. Usage Cited in: Phytomedicine. 2025 Jan 1:136:156330.  [Abstract]

    Punicalagin (PUN)-treated HIE group was administered various doses of PUN (10 and 20 mg/kg) 24 h before hypoxia by intraperitoneal (i.p.) injection at 24 h intervals in Sprague-Dawley (SD) rats. Representative Western blot images and quantification of Bcl-2 and Bax expression three days post-operation in the cortex.

    Punicalagin purchased from MedChemExpress. Usage Cited in: Phytomedicine. 2025 Jan 1:136:156330.  [Abstract]

    Punicalagin (PUN)-treated HIE group was administered various doses of PUN (10 and 20 mg/kg) 24 h before hypoxia by intraperitoneal (i.p.) injection at 24 h intervals in Sprague-Dawley (SD) rats. Representative TUNEL-stained (green) and DAPI-stained (blue) brain sections of the cortex three days post-operatively. Quantitative analysis of the percentage of TUNEL-positive cells in the cortex.

    Punicalagin purchased from MedChemExpress. Usage Cited in: Phytomedicine. 2025 Jan 1:136:156330.  [Abstract]

    Punicalagin (PUN)-treated HIE group was administered various doses of PUN (10 and 20 mg/kg) 24 h before hypoxia by intraperitoneal (i.p.) injection at 24 h intervals in Sprague-Dawley (SD) rats. Representative images of immunohistochemical staining of p-AKT and immunofluorescence staining for p62 (red) and LC3 (green) in the cortex of each group at 72 h post-HI.

    • Biological Activity

    • Purity & Documentation

    • References

    • Customer Review

    Description

    Punicalagin is a polyphenol ingredient isolated from Pomegranate (Punica granatum L.) or the leaves of Terminalia catappa L.. Punicalagin is a reversible and non-competitive 3CLpro inhibitor and inhibits SARS-CoV-2 replication in vitro. Punicalagin is an anti-hepatitis B virus (HBV) agent and has antioxidant, anti-inflammatory, and anticancer effects. Punicalagin has the potential for the research of COVID-19[1][2][3].

    In Vitro

    Punicalagin (100 mg/ml) induces apoptosis in HT-29, HCT116 colon cells[1].
    Punicalagin slightly inhibits the PLpro activity with an IC50 of over 50 μM. Punicalagin inhibits the plaque formation of SARS-CoV-2 in a dose-dependent manner, with EC50 values of 7.20 μM[4].
    Punicalagin binds at an allosteric site in the dimer interface. Punicalagin inhibit SARS-CoV-2 replication by a mechanism other than preventing S-mediated viral entry[4].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Punicalagin Related Antibodies
    In Vivo

    Punicalagin (10 mg/kg) inhibits the edema rate of 58.15% in rats[2].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
    Clinical Trial
    Molecular Weight

    1084.72

    Formula

    C48H28O30
    CAS No.
    Appearance

    Solid

    Color

    Light yellow to yellow

    SMILES

    O=C(C1=C(C2=C(O)C(O)=C(O)C=C2C(O[C@@H]3[C@@H](CO4)OC(O)[C@H](OC5=O)[C@H]3OC(C6=CC(O)=C(O)C(O)=C6C7=C5C=C(O)C(O)=C7O)=O)=O)C(O)=C8O)OC(C(C1=C8O9)=C%10C9=O)=C(O)C(O)=C%10C%11=C(C4=O)C=C(O)C(O)=C%11O
    Structure Classification
    Initial Source
    Shipping

    Room temperature in continental US; may vary elsewhere.
    Storage

    4°C, protect from light

    *In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

    Solvent & Solubility
    In Vitro: 

    H2O : 100 mg/mL (92.19 mM; Need ultrasonic)

    DMSO : 50 mg/mL (46.09 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 0.9219 mL 4.6095 mL 9.2190 mL
    5 mM 0.1844 mL 0.9219 mL 1.8438 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    * Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

    • Molarity Calculator

    • Dilution Calculator

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    Concentration (start)

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    In Vivo:

    Select the appropriate dissolution method based on your experimental animal and administration route.

    For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
    To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

      Solubility: ≥ 2.17 mg/mL (2.00 mM); Clear solution

      This protocol yields a clear solution of ≥ 2.17 mg/mL (saturation unknown).

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (21.7 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

      Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
    • Protocol 2

      Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

      Solubility: ≥ 2.17 mg/mL (2.00 mM); Clear solution

      This protocol yields a clear solution of ≥ 2.17 mg/mL (saturation unknown).

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (21.7 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

      Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

    Dosage

    mg/kg

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    (per animal)

    g

    Dosing volume
    (per animal)

    μL

    Number of animals

    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Calculation results:
    Working solution concentration: mg/mL
    This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
    The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
    Purity & Documentation

    Purity: 99.90%

    SDS (393 KB)

    COA (264 KB) HNMR (389 KB) LCMS (98 KB)

    Handling Instructions (2659 KB)
    References

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    DMSO / H2O 1 mM 0.9219 mL 4.6095 mL 9.2190 mL 23.0474 mL
    5 mM 0.1844 mL 0.9219 mL 1.8438 mL 4.6095 mL
    10 mM 0.0922 mL 0.4609 mL 0.9219 mL 2.3047 mL
    15 mM 0.0615 mL 0.3073 mL 0.6146 mL 1.5365 mL
    20 mM 0.0461 mL 0.2305 mL 0.4609 mL 1.1524 mL
    25 mM 0.0369 mL 0.1844 mL 0.3688 mL 0.9219 mL
    30 mM 0.0307 mL 0.1536 mL 0.3073 mL 0.7682 mL
    40 mM 0.0230 mL 0.1152 mL 0.2305 mL 0.5762 mL
    H2O 50 mM 0.0184 mL 0.0922 mL 0.1844 mL 0.4609 mL
    60 mM 0.0154 mL 0.0768 mL 0.1536 mL 0.3841 mL
    80 mM 0.0115 mL 0.0576 mL 0.1152 mL 0.2881 mL

    * Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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    Punicalagin Related Classifications

    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    Punicalagin65995-63-3SARS-CoVHBVSARS coronavirusHepatitis B virusAntioxidantpolyphenolingredientPomegranateantioxidantanti-inflammatoryanticancerInhibitorinhibitorinhibit

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