2. Apoptosis Anti-infection Neuronal Signaling Epigenetics TGF-beta/Smad Metabolic Enzyme/Protease Cell Cycle/DNA Damage
  3. Apoptosis Influenza Virus Antibiotic Monoamine Oxidase PKC Cytochrome P450 Dopamine β-hydroxylase Reverse Transcriptase Telomerase
  4. Hypericin

Hypericin is a naturally occurring substance found in Hyperlcurn perforatum L. Hypericin is an inhibitor of PKC (protein kinase C), MAO (monoaminoxidase), dopamine-beta-hydroxylase, reverse transcriptase, telomerase and CYP (cytochrome P450). Hypericin shows antitumor, antiviral, antidepressive activities, and can induce apoptosis.

For research use only. We do not sell to patients.

CAS No. : 548-04-9

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Based on 8 publication(s) in Google Scholar

Other Forms of Hypericin:

Hypericin Related Antibodies

Top Publications Citing Use of Products

    Hypericin purchased from MedChemExpress. Usage Cited in: Int J Nanomedicine. 2025 Feb 4:20:1529-1541.  [Abstract]

    WM-266-4 cells were co-cultured with different concentrations of HPDENs for 12 hours. The PBS group served as the negative control, while the Hypericin (0.2 μg/mL) group served as the positive control. Cells were then irradiated with an LED at 4 mW/cm² for 10 minutes or left untreated. Data are expressed as means ± SEM.

    Hypericin purchased from MedChemExpress. Usage Cited in: Int J Nanomedicine. 2025 Feb 4:20:1529-1541.  [Abstract]

    Fluorescence microscopy images depict cellular reactive oxygen species levels following treatment with PBS, Hypericin (0.2 μg/mL), and HPDENs with or without LED illumination. Scale bar: 100 μm. Graphic represents cellular ROS levels observed in fluorescence microscopy images. n=3. Data are expressed as means ± SEM.

    Hypericin purchased from MedChemExpress. Usage Cited in: Int J Nanomedicine. 2025 Feb 4:20:1529-1541.  [Abstract]

    Hypericin (0.2 μg/mL). Flow cytometry was employed to assess the expression of apoptosis-related genes in Annexin V-labeled cells. Quantitation of FITC-positive results obtained through flow cytometry. n=3. Data are expressed as means ± SEM.

    Hypericin purchased from MedChemExpress. Usage Cited in: Biomed Pharmacother. 2023 Nov:167:115545.  [Abstract]

    Hypericin (0.2 mg/kg, gavage). Changes in body weight. (csingle bondh) Behavioral tests: SPT. Quantitation of crossing number of rats in OFT. Rearing number of rats in OFT. The average speed of rats in OFT. Immobility time in FST.

    Hypericin purchased from MedChemExpress. Usage Cited in: Biomed Pharmacother. 2023 Nov:167:115545.  [Abstract]

    Hypericin (0.2 mg/kg, gavage). Western blots and analysis showed that lower levels of Cx43 were restored by HY treatment in CUS rats. Representative bands of Cx43, p-Cx43 at Serine-368, and GAPDH. GAPDH was used as a loading control. Quantitation of Cx43/GAPDH, p-Cx43/GAPDH and p-Cx43/Cx43.

    Hypericin purchased from MedChemExpress. Usage Cited in: Biomed Pharmacother. 2023 Nov:167:115545.  [Abstract]

    Hypericin (0.2 mg/kg, gavage). Immunofluorescent staining of p-Cx43. Cx43 or p-Cx43 was labeled with green fluorescence and nuclei were labeled by Hoechst 33342 with blue fluorescence. Scale bar: 200 µm.

    Hypericin purchased from MedChemExpress. Usage Cited in: Biomed Pharmacother. 2023 Nov:167:115545.  [Abstract]

    Cell viability of CORT-treated astrocytes was improved by Hypericin (0.1, 0.5, 2.5 μM). Results were expressed as percentages of the Ctrl group. Data were obtained from six independent experiments and shown as the mean ± SEM.

    View All Antibiotic Isoform Specific Products:

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    PKC PKCα PKCβ PKCγ PKCδ PKCε PKCη PKCζ PKCθ PKCμ PKC-ι ℩ PKCι

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    CYP1 CYP2 CYP3 CYP4 CYP11 CYP17 Aromatase/CYP19A1 CYP26 CYP51 CYP1A2 CYP2D6 CYP2C9 CYP2C19 CYP3A CYP3A4 CYP17A1

    • Biological Activity

    • Purity & Documentation

    • References

    • Customer Review

    Description

    Hypericin is a naturally occurring substance found in Hyperlcurn perforatum L. Hypericin is an inhibitor of PKC (protein kinase C), MAO (monoaminoxidase), dopamine-beta-hydroxylase, reverse transcriptase, telomerase and CYP (cytochrome P450). Hypericin shows antitumor, antiviral, antidepressive activities, and can induce apoptosis[1][2][3].

    Cellular Effect
    Cell Line Type Value Description References
    CHO IC50
    0.3 μM
    Compound: 1
    Inhibition of recombinant CRF1 receptor expressed in CHO cells assessed as inhibition of CRF-induced cAMP formation
    Inhibition of recombinant CRF1 receptor expressed in CHO cells assessed as inhibition of CRF-induced cAMP formation
    		[PMID: 20408551]
    HaCaT IC50
    1.7 μM
    Compound: 6p ; Hypericin
    Antiproliferative (inhibition of cell growth) activity against HaCaT cells (human keratinocyte line)
    Antiproliferative (inhibition of cell growth) activity against HaCaT cells (human keratinocyte line)
    		[PMID: 9371243]
    In Vitro

    Hypericin (0.25-4 μM; 24 h) inhibits the growth of fibroblasts (Fb), melanocytes (Mc), and keratinocytes (Kc)[2].
    Hypericin (3 μM; 24 h) treatment can induce cells apoptosis[2].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Hypericin Related Antibodies

    Cell Viability Assay[2]

    Cell Line: Fibroblasts (Fb), melanocytes (Mc), and keratinocytes (Kc)
    Concentration: 0.25 μM; 0.5 μM; 1 μM; 2 μM; 3 μM; 4 μM
    Incubation Time: 24 hours
    Result: Showed the LD50 for Fb and Mc at 1.75 μM and 3.5 μM, respectively, and for Kc at a greater dose than 4 μM.

    Apoptosis Analysis[2]

    Cell Line: Fibroblasts (Fb), melanocytes (Mc), and keratinocytes (Kc)
    Concentration: 3 μM
    Incubation Time: 24 hours
    Result: Showed a significant (p<0.001) early apoptotic Fb population (64%), and a smaller, significant (p<0.05) early apoptotic Mc population (20%).
    In Vivo

    Hypericin (Intravenous injection; 10 mg/kg; once) treatment delays tumor growth[3].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Animal Model: 18-20 week-old female BALB/c mice injected with CT26 carcinomas[3]
    Dosage: 10 mg/kg
    Administration: Intravenous injection; 10 mg/kg; once
    Result: Showed a four times delayed tumor growth compared to the control groups.
    Clinical Trial
    Molecular Weight

    504.44

    Formula

    C30H16O8
    CAS No.
    Appearance

    Solid

    Color

    Brown to black

    SMILES

    O=C1C2=C(O)C=C(O)C3=C2C4=C(C5=C6C(O)=CC(C)=C5C7=C4C1=C(O)C=C7C)C8=C3C(O)=CC(O)=C8C6=O
    Structure Classification
    Initial Source
    Shipping

    Room temperature in continental US; may vary elsewhere.
    Storage
    Powder -20°C 3 years
    4°C 2 years
    In solvent -80°C 6 months
    -20°C 1 month
    Solvent & Solubility
    In Vitro: 

    DMSO : 250 mg/mL (495.60 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 1.9824 mL 9.9120 mL 19.8240 mL
    5 mM 0.3965 mL 1.9824 mL 3.9648 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    • Molarity Calculator

    • Dilution Calculator

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    Concentration (start)

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    In Vivo:

    Select the appropriate dissolution method based on your experimental animal and administration route.

    For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
    To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

      Solubility: 2.08 mg/mL (4.12 mM); Suspended solution; Need ultrasonic

      This protocol yields a suspended solution of 2.08 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

      Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
    • Protocol 2

      Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

      Solubility: 2.08 mg/mL (4.12 mM); Clear solution; Need ultrasonic

      This protocol yields a clear solution of 2.08 mg/mL.

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

      Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.

    For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  50% PEG300    50% Saline

      Solubility: 10 mg/mL (19.82 mM); Suspended solution; Need ultrasonic

    • Protocol 2

      Add each solvent one by one:  0.5% CMC-Na/saline water

      Solubility: 25 mg/mL (49.56 mM); Suspended solution; Need ultrasonic

    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

    Dosage

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    g

    Dosing volume
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    Number of animals

    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Please enter your animal formula composition:
    %
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    +
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    Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
    The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
    Calculation results:
    Working solution concentration: mg/mL
    Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
    The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
    Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
     If the continuous dosing period exceeds half a month, please choose this protocol carefully.
    Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
    Purity & Documentation

    Purity: 98.79%

    SDS (393 KB)

    COA (265 KB) HNMR (265 KB) RP-HPLC (205 KB) MS (69 KB)

    Handling Instructions (2659 KB)
    References

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    DMSO 1 mM 1.9824 mL 9.9120 mL 19.8240 mL 49.5599 mL
    5 mM 0.3965 mL 1.9824 mL 3.9648 mL 9.9120 mL
    10 mM 0.1982 mL 0.9912 mL 1.9824 mL 4.9560 mL
    15 mM 0.1322 mL 0.6608 mL 1.3216 mL 3.3040 mL
    20 mM 0.0991 mL 0.4956 mL 0.9912 mL 2.4780 mL
    25 mM 0.0793 mL 0.3965 mL 0.7930 mL 1.9824 mL
    30 mM 0.0661 mL 0.3304 mL 0.6608 mL 1.6520 mL
    40 mM 0.0496 mL 0.2478 mL 0.4956 mL 1.2390 mL
    50 mM 0.0396 mL 0.1982 mL 0.3965 mL 0.9912 mL
    60 mM 0.0330 mL 0.1652 mL 0.3304 mL 0.8260 mL
    80 mM 0.0248 mL 0.1239 mL 0.2478 mL 0.6195 mL
    100 mM 0.0198 mL 0.0991 mL 0.1982 mL 0.4956 mL
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    Hypericin Related Classifications

    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    Hypericin548-04-9ApoptosisInfluenza VirusAntibioticMonoamine OxidasePKCCytochrome P450Dopamine β-hydroxylaseReverse TranscriptaseTelomeraseMAOProtein kinase CCYPsDopamine beta-hydroxylaseDopamine beta-monooxygenaseDopamine β-monooxygenaseHypericumprotein kinase Cmonoaminoxidasedopamine-beta-hydroxylasereverse transcriptasetelomerase and CYPcytochrome P450antitumor activityantiviral activityantidepressive activityapoptosisfibroblastsmelanocyteskeratinocytesInhibitorinhibitorinhibit

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