BLOC1S2 Protein, Human (GST)

BLOC1S2, a vital BLOC-1 complex component, is crucial for lysosome-related organelle (LRO) biogenesis, including platelet dense granules and melanosomes. Collaborating with the AP-3 complex, BLOC-1 directs membrane protein cargos into vesicles for delivery into neurites and nerve terminals, implicating it in neurite extension. As part of the BORC complex, BLOC1S2 contributes to lysosome movement and localization, potentially recruiting ARL8B. It may also influence cell proliferation within the BLOC-1 and BORC complexes, interacting with various components. BLOC1S2 Protein, Human (GST) is the recombinant human-derived BLOC1S2 protein, expressed by E. coli, with N-GST labeled tag. The total length of BLOC1S2 Protein, Human (GST) is 99 a.a., with molecular weight of ~11.35 KDa.

BLOC1S2 protein is a vital component of the BLOC-1 complex, crucial for the normal biogenesis of lysosome-related organelles (LRO), including platelet dense granules and melanosomes. Together with the AP-3 complex, the BLOC-1 complex is essential for directing membrane protein cargos into vesicles formed at cell bodies, facilitating their delivery into neurites and nerve terminals. The BLOC-1 complex, in conjunction with SNARE proteins, is also implicated in neurite extension. As a part of the BORC complex, BLOC1S2 may contribute to the movement and localization of lysosomes at the cell periphery, associating with the cytosolic face of lysosomes and potentially recruiting ARL8B to couple lysosomes to microtubule plus-end-directed kinesin motors. Additionally, BLOC1S2 may play a role in cell proliferation as part of the biogenesis of lysosome-related organelles complex 1 (BLOC-1) and the BLOC-one-related complex (BORC), interacting with various components within these complexes, including BLOC1S1, BLOC1S3, BLOC1S4, BLOC1S5, SNAPIN, and IFT57.

Human

E. coli

N-GST

Q6QNY1-2 (M1-R99)

282991  [NCBI]

Approximately 11.35 kDa.

Greater than 95% as determined by Tris-Bis PAGE.

Lyophilized powder.

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.