DNMT2 Protein, Human (sf9, GST)

The DNMT2 protein exhibits remarkable specificity by selectively methylating cytosine 38 within the anticodon loop of tRNA (Asp). This site-specific methylation is critical for epigenetic modification of transfer RNA, affecting its structure, stability, and functional interactions. DNMT2 Protein, Human (sf9, GST) is the recombinant human-derived DNMT2 protein, expressed by Sf9 insect cells , with N-GST labeled tag. The total length of DNMT2 Protein, Human (sf9, GST) is 391 a.a., with molecular weight of ~60 kDa.

DNMT2 protein exhibits remarkable specificity in its enzymatic activity, as it selectively methylates cytosine 38 within the anticodon loop of tRNA(Asp). This site-specific methylation event plays a crucial role in the epigenetic modification of transfer RNA molecules, potentially influencing their structure, stability, and functional interactions. By targeting a specific cytosine residue in the anticodon loop of tRNA(Asp), DNMT2 contributes to the modulation of translational efficiency and fidelity. The enzyme's precision in methylating this particular site underscores its significance in the intricate regulatory mechanisms governing tRNA function, emphasizing the importance of DNMT2 in the epigenetic landscape of cellular processes involving tRNA(Asp).

Human

Sf9 insect cells

N-GST

O14717 (M1-E391)

1787  [NCBI]

Approximately 60 kDa

Greater than 90% as determined by reducing SDS-PAGE

<1 EU/μg, determined by LAL method.