DNMT2 Protein, Human (sf9, GST)
Based on 1 Customer Validation
The DNMT2 protein exhibits remarkable specificity by selectively methylating cytosine 38 within the anticodon loop of tRNA (Asp). This site-specific methylation is critical for epigenetic modification of transfer RNA, affecting its structure, stability, and functional interactions. DNMT2 Protein, Human (sf9, GST) is the recombinant human-derived DNMT2 protein, expressed by Sf9 insect cells , with N-GST labeled tag.
- Species: Human
- Source: Sf9 insect cells
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Storage:Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
- Help & FAQs
Biological Activity
The DNMT2 protein exhibits remarkable specificity by selectively methylating cytosine 38 within the anticodon loop of tRNA (Asp). This site-specific methylation is critical for epigenetic modification of transfer RNA, affecting its structure, stability, and functional interactions. DNMT2 Protein, Human (sf9, GST) is the recombinant human-derived DNMT2 protein, expressed by Sf9 insect cells , with N-GST labeled tag.
DNMT2 protein exhibits remarkable specificity in its enzymatic activity, as it selectively methylates cytosine 38 within the anticodon loop of tRNA(Asp). This site-specific methylation event plays a crucial role in the epigenetic modification of transfer RNA molecules, potentially influencing their structure, stability, and functional interactions. By targeting a specific cytosine residue in the anticodon loop of tRNA(Asp), DNMT2 contributes to the modulation of translational efficiency and fidelity. The enzyme's precision in methylating this particular site underscores its significance in the intricate regulatory mechanisms governing tRNA function, emphasizing the importance of DNMT2 in the epigenetic landscape of cellular processes involving tRNA(Asp).
The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.
Technical Parameters
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Species Human
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Source Sf9 insect cells
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Tag N-GST
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Accession
O14717 (M1-E391)
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Molecular Construction
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N-term
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GST
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DNMT2 (M1-E391)
Accession # O14717 -
C-term
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Protein Length
Full Length
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Synonyms
TRDMT1; Uncharacterized Protein DKFZp686N1286; Prev. DNMT2; DNA Methyltransferase Homolog HsaIIP; TRNA (Cytosine(38)-C(5))-Methyltransferase; DNA Cytosine-5 Methyltransferase 2; DNA (Cytosine-5)-Methyltransferase-Like Protein 2; DNA Methyltransferase-2; R
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AA Sequence
MEPLRVLELYSGVGGMHHALRESCIPAQVVAAIDVNTVANEVYKYNFPHTQLLAKTIEGITLEEFDRLSFDMILMSPPCQPFTRIGRQGDMTDSRTNSFLHILDILPRLQKLPKYILLENVKGFEVSSTRDLLIQTIENCGFQYQEFLLSPTSLGIPNSRLRYFLIAKLQSEPLPFQAPGQVLMEFPKIESVHPQKYAMDVENKIQEKNVEPNISFDGSIQCSGKDAILFKLETAEEIHRKNQQDSDLSVKMLKDFLEDDTDVNQYLLPPKSLLRYALLLDIVQPTCRRSVCFTKGYGSYIEGTGSVLQTAEDVQVENIYKSLTNLSQEEQITKLLILKLRYFTPKEIANLLGFPPEFGFPEKITVKQRYRLLGNSLNVHVVAKLIKILYE
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Predicted Molecular Mass
71 kDa
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Molecular Weight
Approximately 60 kDa, based on SDS-PAGE under reducing conditions.
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Purity
≥ 90%, as determined by reducing SDS-PAGE.
Product Properties
Lyophilized powder.
Lyophilized from a 0.22 μm filtered solution of 20 mM Tris, 500 mM NaCl, 0.5 mM GSH, 10% glycerol, pH 8, 5% trehalose, 5% mannitol, 0.01% Tween 80 are added as protectants before lyophilization.
Note: For SPR assay, please replace the buffer. Primary amine components (e.g., Tris, imidazole) can affect protein-coupled chips.
<1 EU/μg, determined by LAL method.
It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.
Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
Room temperature in continental US; may vary elsewhere.
Documentation
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Data Sheet (237 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Handling Instructions (2659 KB)
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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