DNMT3A Protein, Human (His, Myc)
Based on 1 Customer Validation
- Species: Human
- Source: E. coli
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Storage:Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
Technical Parameters
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Species Human
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Source E. coli
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Tag N-10*His;C-Myc
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Accession
Q9Y6K1-3 (M1-P166)
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Gene ID1788
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Molecular Construction
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N-term
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10*His
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DNMT3A (M1-P166)
Accession # Q9Y6K1-3 -
Myc
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C-term
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Protein Length
Partial
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Synonyms
DNMT3A; DNA (Cytosine-5-)-Methyltransferase; DNA Methyltransferase 3 Alpha; DNA Cytosine Methyltransferase 3A2; DNA (Cytosine-5)-Methyltransferase 3A; DNA Methyltransferase HsaIIIA; Cysteine Methyltransferase DNMT3A; DNMT3A2; DNA (Cytosine-5-)-Methyltrans
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AA Sequence
MPAMPSSGPGDTSSSAAEREEDRKDGEEQEEPRGKEERQEPSTTARKVGRPGRKRKHPPVESGDTPKDPAVISKSPSMAQDSGASELLPNGDLEKRSEPQPEEGSPAGGQKGGAPAEGEGAAETLPEASRAVENGCCTPKEGRGAPAEAGESSAPGAASSGPTSIP
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Predicted Molecular Mass
24.3 kDa
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Molecular Weight
Approximately 35 kDa, based on SDS-PAGE under reducing conditions.
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Structure/Form
Monomer
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Purity
≥ 90%, as determined by reducing SDS-PAGE.
Product Properties
Lyophilized powder.
1.Lyophilized from a 0.22 μm filtered solution of Tris/PBS-based buffer, 6% Trehalose.
2.Lyophilized from a 0.22 μm filtered solution of PBS, 6% Trehalose, pH 7.4.
Note: For SPR assay, please replace the buffer. Primary amine components (e.g., Tris, imidazole) can affect protein-coupled chips.
Please refer to the lot-specific COA for specific buffer information.
<1 EU/μg, determined by LAL method.
It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).
Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
Room temperature in continental US; may vary elsewhere.
Documentation
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Data Sheet (233 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Handling Instructions (2659 KB)
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)