KRT35 Protein, Human (His)
KRT35 Protein shows early expression in the hair follicle, primarily localized in supramatricial and lowermost cortical cells of the hair bulb. KRT35 Protein, Human (His) is the recombinant human-derived KRT35 protein, expressed by E. coli , with N-6*His labeled tag.
- Species: Human
- Source: E. coli
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Storage:Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
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Biological Activity
KRT35 Protein shows early expression in the hair follicle, primarily localized in supramatricial and lowermost cortical cells of the hair bulb. KRT35 Protein, Human (His) is the recombinant human-derived KRT35 protein, expressed by E. coli , with N-6*His labeled tag.
KRT35 Protein exhibits early expression in the hair follicle, predominantly localized in supramatricial cells and the lowermost cortical cells of the hair bulb.
Technical Parameters
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Species Human
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Source E. coli
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Tag N-6*His
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Accession
Q92764 (M1-F455)
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Gene ID3886 [NCBI]
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Molecular Construction
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N-term
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6*His
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KRT35 (M1-K483)
Accession # P05787 -
C-term
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Protein Length
Full Length
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Synonyms
KRT35; HHa5 Hair Keratin Type I Intermediate Filament; Prev. KRTHA5; Hard Keratin Type I 5; Keratin, Type I Cuticular Ha5; Keratin-35; Ha-5; HHa5; Keratin, Hair, Acidic, 5; HKA5; Hair Keratin, Type I Ha5; HHA5; Keratin 35, Type I; HA5; K35; Keratin 35; Ha
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AA Sequence
MASKCLKAGFSSGSLKSPGGASGGSTRVSAMYSSSSCKLPSLSPVARSFSACSVGLGRSSYRATSCLPALCLPAGGFATSYSGGGGWFGEGILTGNEKETMQSLNDRLAGYLEKVRQLEQENASLESRIREWCEQQVPYMCPDYQSYFRTIEELQKKTLCSKAENARLVVEIDNAKLAADDFRTKYETEVSLRQLVESDINGLRRILDDLTLCKSDLEAQVESLKEELLCLKKNHEEEVNSLRCQLGDRLNVEVDAAPPVDLNRVLEEMRCQYETLVENNRRDAEDWLDTQSEELNQQVVSSSEQLQSCQAEIIELRRTVNALEIELQAQHSMRDALESTLAETEARYSSQLAQMQCMITNVEAQLAEIRADLERQNQEYQVLLDVRARLECEINTYRGLLESEDSKLPCNPCAPDYSPSKSCLPCLPAASCGPSAARTNCSPRPICVPCPGGRF
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Predicted Molecular Mass
52 kDa
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Molecular Weight
Approximately 52 kDa, based on SDS-PAGE under reducing conditions.
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Purity
≥ 90%, as determined by reducing SDS-PAGE.
Product Properties
Lyophilized powder.
<1 EU/μg, determined by LAL method.
It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).
Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
Room temperature in continental US; may vary elsewhere.
Documentation
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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