NrdD Protein, E.coli (His)
Based on 1 Customer Validation
The NrdD protein plays a key role in cellular processes by catalyzing the conversion of ribonucleotides into deoxyribonucleotides, an essential component required for DNA synthesis and repair. Various studies have demonstrated that NrdD contributes to the maintenance and replication of genetic material by promoting the conversion of ribonucleotides into their deoxyribonucleotide counterparts through its enzymatic activity. NrdD Protein, E.coli (His) is the recombinant E. coli-derived NrdD protein, expressed by E. coli , with N-His labeled tag.
- Species: E.coli
- Source: E. coli
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Storage:Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
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Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
- Help & FAQs
Biological Activity
The NrdD protein plays a key role in cellular processes by catalyzing the conversion of ribonucleotides into deoxyribonucleotides, an essential component required for DNA synthesis and repair. Various studies have demonstrated that NrdD contributes to the maintenance and replication of genetic material by promoting the conversion of ribonucleotides into their deoxyribonucleotide counterparts through its enzymatic activity. NrdD Protein, E.coli (His) is the recombinant E. coli-derived NrdD protein, expressed by E. coli , with N-His labeled tag.
NrdD is an enzyme that plays a crucial role in nucleotide metabolism by catalyzing the conversion of ribonucleotides into deoxyribonucleotides. This enzymatic activity is essential for DNA synthesis and repair processes within the cell. NrdD contributes to the balance of deoxyribonucleotide pools necessary for the accurate and efficient replication of DNA. The enzyme is capable of reducing each of the four common ribonucleoside triphosphates, underscoring its versatility in modifying a range of nucleotide substrates. It has to highlight NrdD's central role in nucleotide biosynthesis, emphasizing its significance in providing the precursors required for the synthesis and maintenance of DNA, which is fundamental for various cellular processes.
The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.
Technical Parameters
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Species E.coli
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Source E. coli
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Tag N-His
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Accession
P28903 (M1-G712)
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Gene ID948755 [NCBI]
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Molecular Construction
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N-term
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His
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NrdD (M1-G712)
Accession # P28903 -
C-term
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Protein Length
Full Length
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Synonyms
Anaerobic ribonucleoside-triphosphate reductase; EC:1.1.98.6; Class III ribonucleoside-triphosphate reductase; nrdD; b4238; JW4197
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AA Sequence
MTPHVMKRDGCKVPFKSERIKEAILRAAKAAEVDDADYCATVAAVVSEQMQGRNQVDINEIQTAVENQLMSGPYKQLARAYIEYRHDRDIEREKRGRLNQEIRGLVEQTNASLLNENANKDSKVIPTQRDLLAGIVAKHYARQHLLPRDVVQAHERGDIHYHDLDYSPFFPMFNCMLIDLKGMLTQGFKMGNAEIEPPKSISTATAVTAQIIAQVASHIYGGTTINRIDEVLAPFVTASYNKHRKTAEEWNIPDAEGYANSRTIKECYDAFQSLEYEVNTLHTANGQTPFVTFGFGLGTSWESRLIQESILRNRIAGLGKNRKTAVFPKLVFAIRDGLNHKKGDPNYDIKQLALECASKRMYPDILNYDQVVKVTGSFKTPMGCRSFLGVWENENGEQIHDGRNNLGVISLNLPRIALEAKGDEATFWKLLDERLVLARKALMTRIARLEGVKARVAPILYMEGACGVRLNADDDVSEIFKNGRASISLGYIGIHETINALFGGEHVYDNEQLRAKGIAIVERLRQAVDQWKEETGYGFSLYSTPSENLCDRFCRLDTAEFGVVPGVTDKGYYTNSFHLDVEKKVNPYDKIDFEAPYPPLANGGFICYGEYPNIQHNLKALEDVWDYSYQHVPYYGTNTPIDECYECGFTGEFECTSKGFTCPKCGNHDASRVSVTRRVCGYLGSPDARPFNAGKQEEVKRRVKHLGNGQIG
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Predicted Molecular Mass
84 kDa
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Purity
≥ 90%, as determined by reducing SDS-PAGE.
Product Properties
Lyophilized powder.
Lyophilized from a 0.22 μm filtered solution of PBS, 6% trehalose, pH 7.4.
<1 EU/μg, determined by LAL method.
It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.
Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
Room temperature in continental US; may vary elsewhere.
Documentation
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Data Sheet (239 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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