OLFML3 Protein, Human (HEK293, His)
OLFML3 Protein, Human (HEK293, His) is the recombinant human-derived OLFML3 protein, expressed by HEK293, with C-His tag.
- Species: Human
- Source: HEK293
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Storage:Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
- Help & FAQs
Biological Activity
OLFML3 Protein, Human (HEK293, His) is the recombinant human-derived OLFML3 protein, expressed by HEK293, with C-His tag.
OLFML3 is a secreted scaffold protein essential for dorsoventral patterning during early development. It stabilizes axial formation by restricting chordin (CHRD) activity on the dorsal side, achieved through facilitating the association between tolloid proteases and their substrate chordin (CHRD), thereby enhancing chordin (CHRD) degradation (By similarity). It may also have matrix-related functions involved in placental and embryonic development or play similar roles in other physiological processes.
Technical Parameters
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Species Human
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Source HEK293
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Tag C-His
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Accession
Q9NRN5-1 (K120-V406)
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Gene ID56944
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Molecular Construction
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N-term
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OLFML3 (K120-V406)
Accession # Q9NRN5-1 -
His
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C-term
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Protein Length
Partial
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Synonyms
OLFML3; CDNA FLJ58142, Highly Similar To Olfactomedin-Like Protein 3; Olfactomedin Like 3; Olfactomedin-Like 3, Isoform CRA_b; HNOEL-Iso; Olfactomedin-Like 3; Olfactomedin-Like Protein 3; Olfactomedin, 44-Kd; OLF44; HOLF44
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AA Sequence
KGKGRRNEKYDMVTDCGYTISQVRSMKILKRFGGPAGLWTKDPLGQTEKIYVLDGTQNDTAFVFPRLRDFTLAMAARKASRVRVPFPWVGTGQLVYGGFLYFARRPPGRPGGGGEMENTLQLIKFHLANRTVVDSSVFPAEGLIPPYGLTADTYIDLAADEEGLWAVYATREDDRHLCLAKLDPQTLDTEQQWDTPCPRENAEAAFVICGTLYVVYNTRPASRARIQCSFDASGTLTPERAALPYFPRRYGAHASLRYNPRERQLYAWDDGYQIVYKLEMRKKEEEV
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Predicted Molecular Mass
34.4 kDa
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Molecular Weight
Approximately 38-45 kDa, based on SDS-PAGE under reducing conditions, due to the glycosylation.
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Glycosylation
Yes
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Structure/Form
Monomer
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Purity
≥ 95%, as determined by reducing SDS-PAGE.
Product Properties
Lyophilized powder.
<1 EU/μg, determined by LAL method.
It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).
Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
Room temperature in continental US; may vary elsewhere.
Documentation
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Data Sheet (236 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Handling Instructions (2659 KB)
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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