RFK Protein, Human (His)
Based on 1 Customer Validation
RFK protein plays a key role in cellular metabolism by catalyzing the phosphorylation of riboflavin (vitamin B2) to generate flavin mononucleotide (FMN), making it the rate-limiting enzyme in FAD synthesis. This enzyme activity is critical for tumor necrosis factor (TNF)-induced reactive oxygen species (ROS) generation. RFK Protein, Human (His) is the recombinant human-derived RFK protein, expressed by E. coli , with N-His labeled tag.
- Species: Human
- Source: E. coli
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Storage:Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
- Help & FAQs
Biological Activity
RFK protein plays a key role in cellular metabolism by catalyzing the phosphorylation of riboflavin (vitamin B2) to generate flavin mononucleotide (FMN), making it the rate-limiting enzyme in FAD synthesis. This enzyme activity is critical for tumor necrosis factor (TNF)-induced reactive oxygen species (ROS) generation. RFK Protein, Human (His) is the recombinant human-derived RFK protein, expressed by E. coli , with N-His labeled tag.
The RFK protein serves as the rate-limiting enzyme in the synthesis of flavin adenine dinucleotide (FAD) by catalyzing the phosphorylation of riboflavin (vitamin B2) to form flavin-mononucleotide (FMN). This enzymatic activity is essential for the production of FAD, a crucial cofactor involved in various redox reactions within cells. Beyond its role in riboflavin metabolism, RFK has been implicated in the regulation of reactive oxygen species (ROS) production induced by tumor necrosis factor (TNF). Through its interaction with TNFRSF1A and CYBA, RFK physically and functionally couples TNFRSF1A to NADPH oxidase, a key enzyme complex in ROS generation. TNF activation of RFK may enhance the incorporation of FAD in NADPH oxidase, highlighting the importance of RFK in orchestrating critical steps for the assembly and activation of this oxidative stress-inducing enzyme complex.
Measured by its ability to combine with the substrate ATP and riboflavin reaction produces the ADP ability to measure. The specific activity is 3681.943 pmoL/min/μg, as measured under the described conditions.
Technical Parameters
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Species Human
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Source E. coli
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Tag N-His
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Accession
Q969G6 (M1-H155)
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Molecular Construction
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N-term
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His
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RFK (M1-H155)
Accession # Q969G6 -
C-term
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Protein Length
Full Length
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Synonyms
RFK; Flavokinase; Riboflavin Kinase; FLJ11149; RIFK; 0610038L10Rik; ATP:Riboflavin 5'-Phosphotransferase
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AA Sequence
MRHLPYFCRGQVVRGFGRGSKQLGIPTANFPEQVVDNLPADISTGIYYGWASVGSGDVHKMVVSIGWNPYYKNTKKSMETHIMHTFKEDFYGEILNVAIVGYLRPEKNFDSLESLISAIQGDIEEAKKRLELPEHLKIKEDNFFQVSKSKIMNGH
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Molecular Weight
Approximately 20 kDa, based on SDS-PAGE under reducing conditions.
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Purity
≥ 95%, as determined by reducing SDS-PAGE.
Product Properties
Solution
Supplied as a 0.22 μm filtered solution of 50 mM Tris-HCl, 300 mM NaCl, pH 8.0, 10% glycerol.
Note: For SPR assay, please replace the buffer. Primary amine components (e.g., Tris, imidazole) can affect protein-coupled chips.
<1 EU/μg, determined by LAL method.
Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
Shipping with dry ice.
Documentation
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Data Sheet (236 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Handling Instructions (2659 KB)
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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