ZRANB1 Protein, Human (sf9)
ZRANB1 is a ubiquitin thioesterase that specifically hydrolyzes “Lys-29”-linked and “Lys-33”-linked diubiquitins, with additional activity on “Lys-63”-linked chains. It positively regulates the Wnt pathway by deubiquitinating APC, an inhibitor of Wnt-mediated transcription. ZRANB1 Protein, Human (sf9) is the recombinant human-derived ZRANB1 protein, expressed by sf9 insect cells , with tag free.
- Species: Human
- Source: Sf9 insect cells
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Storage:Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
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Biological Activity
ZRANB1 is a ubiquitin thioesterase that specifically hydrolyzes “Lys-29”-linked and “Lys-33”-linked diubiquitins, with additional activity on “Lys-63”-linked chains. It positively regulates the Wnt pathway by deubiquitinating APC, an inhibitor of Wnt-mediated transcription. ZRANB1 Protein, Human (sf9) is the recombinant human-derived ZRANB1 protein, expressed by sf9 insect cells , with tag free.
ZRANB1, functioning as a ubiquitin thioesterase, demonstrates specificity in hydrolyzing 'Lys-29'-linked and 'Lys-33'-linked diubiquitin, with additional activity in cleaving 'Lys-63'-linked chains, albeit less efficiently. Notably, ZRANB1 acts as a positive regulator of the Wnt signaling pathway by deubiquitinating APC protein, a negative regulator of Wnt-mediated transcription. Moreover, it plays a pivotal role in autophagy regulation through the deubiquitination of PIK3C3/VPS34, facilitating autophagosome maturation. Beyond its involvement in intracellular processes, ZRANB1 is implicated in the modulation of cell morphology and cytoskeletal organization, contributing to stress fiber dynamics and cell migration.
Technical Parameters
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Species Human
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Source Sf9 insect cells
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Tag Tag Free
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Accession
Q9UGI0 (M1-E708)
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Gene ID54764
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Molecular Construction
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N-term
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ZRANB1 (M1-E708)
Accession # Q9UGI0 -
C-term
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Protein Length
Full Length
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Synonyms
ZRANB1; TRAF-Binding Domain-Containing Protein; Zinc Finger RANBP2-Type Containing 1; Ubiquitin Thioesterase ZRANB1; TRABID; HTrabid; Zinc Finger Ran-Binding Domain-Containing Protein 1; TRAF-Binding Protein Domain; Zinc Finger, RAN-Binding Domain Contain
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AA Sequence
MSERGIKWACEYCTYENWPSAIKCTMCRAQRPSGTIITEDPFKSGSSDVGRDWDPSSTEGGSSPLICPDSSARPRVKSSYSMENANKWSCHMCTYLNWPRAIRCTQCLSQRRTRSPTESPQSSGSGSRPVAFSVDPCEEYNDRNKLNTRTQHWTCSVCTYENWAKAKRCVVCDHPRPNNIEAIELAETEEASSIINEQDRARWRGSCSSGNSQRRSPPATKRDSEVKMDFQRIELAGAVGSKEELEVDFKKLKQIKNRMKKTDWLFLNACVGVVEGDLAAIEAYKSSGGDIARQLTADEVRLLNRPSAFDVGYTLVHLAIRFQRQDMLAILLTEVSQQAAKCIPAMVCPELTEQIRREIAASLHQRKGDFACYFLTDLVTFTLPADIEDLPPTVQEKLFDEVLDRDVQKELEEESPIINWSLELATRLDSRLYALWNRTAGDCLLDSVLQATWGIYDKDSVLRKALHDSLHDCSHWFYTRWKDWESWYSQSFGLHFSLREEQWQEDWAFILSLASQPGASLEQTHIFVLAHILRRPIIVYGVKYYKSFRGETLGYTRFQGVYLPLLWEQSFCWKSPIALGYTRGHFSALVAMENDGYGNRGAGANLNTDDDVTITFLPLVDSERKLLHVHFLSAQELGNEEQQEKLLREWLDCCVTEGGVLVAMQKSSRRRNHPLVTQMVEKWLDRYRQIRPCTSLSDGEEDEDDEDE
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Purity
≥ 90%, as determined by reducing SDS-PAGE.
Product Properties
Solution.
<1 EU/μg, determined by LAL method.
Please use rapid thawing with running water to thaw the protein.
Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
Shipping with dry ice.
Documentation
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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