Ro 08-2750

Name: Ro 08-2750
Brand: MedChemExpress
SKU: HY-108466
Rating: 4.5 (2 reviews)

Cat. No.: HY-108466 Purity: 98.63%: FAQs
Data Sheet SDS COA Handling Instructions

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Ro 08-2750 is a non-peptide and reversible nerve growth factor (NGF) inhibitor which binds to NGF, and with an IC₅₀ of ~ 1 µM. Ro 08-2750 inhibits NGF binding to p75^NTR selectively over TRKA. Ro 08-2750 is a selective _{MSI RNA-binding} activity inhibitor, with an IC₅₀ of 2.7 μM.

For research use only. We do not sell to patients.

Ro 08-2750 Chemical Structure

CAS No. : 37854-59-4

Size	Price	Stock	Quantity
Solid + Solvent
10 mM * 1 mL in DMSO ready for reconstitution	USD 83	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	USD 83	In-stock	Estimated Time of Arrival: December 31
Solid
5 mg	USD 75	In-stock	Estimated Time of Arrival: December 31
10 mg	USD 120	In-stock	Estimated Time of Arrival: December 31
25 mg	USD 240	In-stock	Estimated Time of Arrival: December 31
50 mg	USD 380	In-stock	Estimated Time of Arrival: December 31
100 mg		Get quote
200 mg		Get quote

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This product is a controlled substance and not for sale in your territory.

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Based on 2 publication(s) in Google Scholar

2 Publications Citing Use of MCE Ro 08-2750

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Description

Ro 08-2750 is a non-peptide and reversible nerve growth factor (NGF) inhibitor which binds to NGF, and with an IC₅₀ of ~ 1 µM. Ro 08-2750 inhibits NGF binding to p75^NTR selectively over TRKA^[1]. Ro 08-2750 is a selective _{MSI RNA-binding} activity inhibitor, with an IC₅₀ of 2.7 μM^[3].

IC₅₀ & Target

IC50: ~1 µM (NGF)^[1], 2.7 μM (MSI RNA-binding)^[3]

In Vitro

Ro 08-2750 binds to the NGF dimer thereby probably inducing a change in its conformation such that NGF cannot bind to p75^NTR anymore^[2].
? Ro 08-2750 (10 nM) completely rescues cells from undergoing NGF-induced SK-N-MC 103 cells death^[2].
? Ro 08-2750 (5-10 μM; 8?hours) increases differentiation and apoptosis in myeloid leukemia cells^[3].
? Ro 08-2750 inhibits survival of human AML lines and patient cells^[3].
? Ro 08-2750 inhibits MSI2 RNA-binding and alters MSI2 gene signature^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Apoptosis Analysis^[3]

Cell Line:	MLL-AF9 + BM cells
Concentration:	5 μM, 10 μM
Incubation Time:	8 hours
Result:	Increased apoptosis.

In Vivo

Ro 08-2750 (13.75?mg/kg; i.p.) inhibits leukemogenesis in a myeloid leukemia model in vivo^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	C57BL/6 wild type mice (10-12-weeks-old), MLL-AF9 murine leukemia model^[3]
Dosage:	13.75 mg/kg
Administration:	Intraperitoneal injection, at days 1, 4, 7, 10, and 13 (one day on, two days off drug)
Result:	Inhibited c-MYC levels and reduced disease burden.

Molecular Weight

270.24

Formula

C₁₃H₁₀N₄O₃

CAS No.

37854-59-4

Appearance

Solid

Color

Light yellow to orange

SMILES

O=CC1=C(C)C=C2N=C3C(NC(N=C3N(C)C2=C1)=O)=O

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	2 years
	-20°C	1 year

Solvent & Solubility

In Vitro:

DMSO : 4 mg/mL (14.80 mM; ultrasonic and warming and heat to 80°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	3.7004 mL	18.5021 mL	37.0041 mL
5 mM	0.7401 mL	3.7004 mL	7.4008 mL
10 mM	0.3700 mL	1.8502 mL	3.7004 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

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Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

In Vivo:

For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 50% PEG300 50% Saline
Solubility: 5 mg/mL (18.50 mM); Suspended solution; Need ultrasonic
Protocol 2

Add each solvent one by one: 0.5% CMC-Na/saline water
Solubility: 5 mg/mL (18.50 mM); Suspended solution; Need ultrasonic

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

Purity & Documentation

Purity: 98.63%

Select Batch:

Data Sheet (276 KB) SDS (0 KB)

COA (249 KB) HNMR (161 KB) RP-HPLC (246 KB) MS (162 KB)

Handling Instructions (2659 KB)

References

[1]. Arkin MR, et al. Small-molecule inhibitors of protein-protein interactions: progressing towards the dream. Nat Rev Drug Discov. 2004 Apr;3(4):301-17. [Content Brief]

[2]. Niederhauser O, et al. NGF ligand alters NGF signaling via p75(NTR) and trkA. J Neurosci Res. 2000 Aug 1;61(3):263-72. [Content Brief]

[3]. Minuesa G, et al. Small-molecule targeting of MUSASHI RNA-binding activity in acute myeloid leukemia. Nat Commun. 2019 Jun 19;10(1):2691. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	3.7004 mL	18.5021 mL	37.0041 mL	92.5104 mL
	5 mM	0.7401 mL	3.7004 mL	7.4008 mL	18.5021 mL
	10 mM	0.3700 mL	1.8502 mL	3.7004 mL	9.2510 mL

Ro 08-2750 Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Ro 08-275037854-59-4ApoptosisNervegrowthfactorNGFp75NTRTRKAMSIRNA-bindingMUSASHIAMLleukemiaInhibitorinhibitorinhibit

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Ro 08-2750

Customer Review

Ro 08-2750 Related Antibodies

2 Publications Citing Use of MCE Ro 08-2750

Featured Recommendations

•Related Screening Libraries:

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Biological Activity

Purity & Documentation

References

Customer Review

Ro 08-2750 Related Antibodies

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Complete Stock Solution Preparation Table

Ro 08-2750 Related Classifications

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