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Ro 08-2750 

Cat. No.: HY-108466 Purity: 95.01%
Handling Instructions

Ro 08-2750 is a non-peptide and reversible nerve growth factor (NGF) inhibitor which binds to NGF, and with an IC50 of ~ 1 µM. Ro 08-2750 inhibits NGF binding to p75NTR selectively over TRKA. Ro 08-2750 is a selective MSI RNA-binding activity inhibitor, with an IC50 of 2.7 μM.

For research use only. We do not sell to patients.

Ro 08-2750 Chemical Structure

Ro 08-2750 Chemical Structure

CAS No. : 37854-59-4

Size Price Stock Quantity
10 mM * 1 mL in DMSO USD 461 In-stock
Estimated Time of Arrival: December 31
1 mg USD 155 In-stock
Estimated Time of Arrival: December 31
5 mg USD 465 In-stock
Estimated Time of Arrival: December 31
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Based on 1 publication(s) in Google Scholar

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Description

Ro 08-2750 is a non-peptide and reversible nerve growth factor (NGF) inhibitor which binds to NGF, and with an IC50 of ~ 1 µM. Ro 08-2750 inhibits NGF binding to p75NTR selectively over TRKA[1]. Ro 08-2750 is a selective MSI RNA-binding activity inhibitor, with an IC50 of 2.7 μM[3].

IC50 & Target

IC50: ~1 µM (NGF)[1], 2.7 μM (MSI RNA-binding)[3]

In Vitro

Ro 08-2750 binds to the NGF dimer thereby probably inducing a change in its conformation such that NGF cannot bind to p75NTR anymore[2].
Ro 08-2750 (10 nM) completely rescues cells from undergoing NGF-induced SK-N-MC 103 cells death[2].
Ro 08-2750 (5-10 μM; 8 hours) increases differentiation and apoptosis in myeloid leukemia cells[3].
Ro 08-2750 inhibits survival of human AML lines and patient cells[3].
Ro 08-2750 inhibits MSI2 RNA-binding and alters MSI2 gene signature[3].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Apoptosis Analysis[3]

Cell Line: MLL-AF9 + BM cells
Concentration: 5 μM, 10 μM
Incubation Time: 8 hours
Result: Increased apoptosis.
In Vivo

Ro 08-2750 (13.75 mg/kg; i.p.) inhibits leukemogenesis in a myeloid leukemia model in vivo[3].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: C57BL/6 wild type mice (10-12-weeks-old), MLL-AF9 murine leukemia model[3]
Dosage: 13.75 mg/kg
Administration: Intraperitoneal injection, at days 1, 4, 7, 10, and 13 (one day on, two days off drug)
Result: Inhibited c-MYC levels and reduced disease burden.
Molecular Weight

270.24

Formula

C₁₃H₁₀N₄O₃

CAS No.

37854-59-4

SMILES

O=CC1=C(C)C=C2N=C3C(NC(N=C3N(C)C2=C1)=O)=O

Shipping

Room temperature in continental US; may vary elsewhere.

Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : 5 mg/mL (18.50 mM; ultrasonic and warming and heat to 60°C)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 3.7004 mL 18.5021 mL 37.0041 mL
5 mM 0.7401 mL 3.7004 mL 7.4008 mL
10 mM 0.3700 mL 1.8502 mL 3.7004 mL
*Please refer to the solubility information to select the appropriate solvent.
References
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Keywords:

Ro 08-2750ApoptosisNervegrowthfactorNGFp75NTRTRKAMSIRNA-bindingMUSASHIAMLleukemiaInhibitorinhibitorinhibit

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Ro 08-2750
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HY-108466
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