Pleurocidin
Based on 1 publication(s) in Google Scholar
Pleurocidin is an Antimicrobial peptide. Pleurocidin is derived from the skin mucosa or intestinal secretions of Pseudopleuronectes americanus. Pleurocidin inhibits the expression of key proteins in the MAPK and NF-κB inflammatory signaling pathways. Pleurocidin alters serum inflammatory and immune cytokine levels, regulates the down-regulation of tight junction proteins, and modulates the intestinal flora. Pleurocidin exerts antibacterial activity by inducing bacterial membrane damage, hydroxyl radical formation, and NADH depletion, and also produces a synergistic effect with Antibiotics. Pleurocidin alleviates DSS-induced ulcerative colitis. Pleurocidin can be used for research on ulcerative colitis, bacterial infections, and bacterial biofilm-related infections.
For research use only. We do not sell to patients.
- Purity: 98.91%
- CAS No.: 190324-47-1
- Formula: C129H192N36O29
- Molecular Weight:2711.13
-
Storage:
Sealed storage, away from moisture.
Powder -80°C, 2 years , -20°C, 1 year* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
-
Biological Activity
-
Chemical Information
-
Solvent & Solubility
- Purity & Documentation
- References
-
Help & FAQs
Publications Citing Use of MedChemExpress (MCE) Pleurocidin
MoreAll Antibiotic Isoforms
More
Biological Activity
|
p38 MAPK |
Pleurocidin triggers the expression of upstream genes related to IL-1β and COX-2 in rainbow trout macrophages[1].
Pleurocidin exhibits antibacterial activity against Escherichia coli in bacterial lytic plate assays[2].
Pleurocidin (18 h) inhibits the growth of planktonic S. aureus ATCC 25923, E. faecium ATCC 19434, P. acnes ATCC 6919, E. coli ATCC 25922, E. coli O-157 ATCC 43895, and P. aeruginosa ATCC 27853, with an MIC of 1.9-3.8 μg/mL after incubation at 37°C for 18 h[3].
At its minimum inhibitory concentration (MIC), Pleurocidin (MIC; 30-120 min) transiently increases the NAD+/NADH ratio of planktonic S. aureus ATCC 25923 and P. aeruginosa ATCC 27853 to more than 4 times the original level at 30 min post-treatment, and this ratio returns to the baseline level at 60 min[3].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
-
Animal Model:BALB/C6J (male, 7 weeks old, 16-20 g, DSS-induced colitis)[1]
-
Dosage:5 mg/kg
-
Administration:rectal injection; 4 doses (days 8, 10, 12, 14 of trial)
-
Result:Reduced body weight loss, lower disease activity index (DAI) score, longer colon length, and reduced rectal bleeding compared to DSS-only mice.
Significantly lowered histological scores, reduced crypt loss and intestinal villi destruction, increased goblet cell numbers, reduced epithelial cell apoptosis, and decreased colonic tissue infiltration of F4/80-positive macrophages and CD177-positive neutrophils compared to DSS-only mice.
Reduced levels of pro-inflammatory cytokines IL-1β, IL-6, TNF-α, and IFN-γ, and restored levels of anti-inflammatory cytokine IL-10 compared to DSS-only mice.
Restored colonic expression of tight junction proteins ZO-1, occludin, and claudin-1, and reduced serum levels of intestinal permeability markers LPS, D-LA, and DAO compared to DSS-only mice.
Reduced colonic tissue expression of phosphorylated NF-κB p65, p38 MAPK, JNK, and ERK proteins compared to DSS-only mice.
Increased α-diversity (Ace, Chao, and Sobs indices) compared to DSS-only mice, shifted microbiota composition closer to healthy controls, reduced relative abundance of harmful taxa (Gammaproteobacteria, Enterobacteriales, Escherichia), and increased relative abundance of beneficial taxa including Butyricicoccus and Saccharibacteria compared to DSS-only mice.
Chemical Information
-
CAS No. 190324-47-1
-
Appearance Solid
-
Molecular Weight 2711.13
-
Formula C129H192N36O29
-
Color White to off-white
-
Sequence
Gly-Trp-Gly-Ser-Phe-Phe-Lys-Lys-Ala-Ala-His-Val-Gly-Lys-His-Val-Gly-Lys-Ala-Ala-Leu-Thr-His-Tyr-Leu
-
Sequence Shortening
GWGSFFKKAAHVGKHVGKAALTHYL
-
Shipping
Room temperature in continental US; may vary elsewhere.
-
Storage
Sealed storage, away from moisture
Powder -80°C 2 years -20°C 1 year * In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
Publications (1)
-
Journal Impact Factor
-
Most Recent
Solvent & Solubility
H2O : ≥ 50 mg/mL (18.44 mM)
* "≥" means soluble, but saturation unknown.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Purity & Documentation
-
Data Sheet (276 KB)
-
SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Portuguese - PT (252 KB)
-
Handling Instructions (2659 KB)
References
[1]. Nong K, et al. Effect of the Pseudopleuronectes americanus-derived Pleurocidin on DSS-induced Ulcerative colitis in mice and its preliminary molecular mechanisms. Int Immunopharmacol. 2024;130:111757. [Content Brief]
[2]. Cole AM, et al. Isolation and characterization of pleurocidin, an antimicrobial peptide in the skin secretions of winter flounder. J Biol Chem. 1997;272(18):12008-12013. [Content Brief]
[3]. Choi H, et al. Antimicrobial peptide pleurocidin synergizes with antibiotics through hydroxyl radical formation and membrane damage, and exerts antibiofilm activity. Biochim Biophys Acta. 2012;1820(12):1831-1838. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| H2O | 1 mM | 0.3688 mL | 1.8442 mL | 3.6885 mL | 9.2212 mL |
| 5 mM | 0.0738 mL | 0.3688 mL | 0.7377 mL | 1.8442 mL | |
| 10 mM | 0.0369 mL | 0.1844 mL | 0.3688 mL | 0.9221 mL | |
| 15 mM | 0.0246 mL | 0.1229 mL | 0.2459 mL | 0.6147 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Powered by Bioz