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TAT-CN21 is a potent CaMKII inhibitor with an IC50 of 77.2 nM. TAT-CN21 inhibits both calcium/calmodulin-dependent and autonomously activated CaMKII, blocks glutamate-induced translocation of CaMK IIα, and reverses the enhanced phosphorylation of CaMKII at Thr286 following excitotoxic injury. TAT-CN21 shows application potential in studies related to ischemic stroke by reducing neuronal excitotoxicity and exacerbating pre-existing long-term neuronal death prior to injury. TAT-CN21 improves definitive behaviors in rats with residual nerve injury without altering indicators such as mechanical/thermal hyperalgesia or spatial memory. TAT-CN21 can also be used in studies related to neuropathic pain .
Autocamtide 2 is a highly selective peptide substrate of calcium/calmodulin-dependent protein kinase II (CaMKII). It can be used in the CaMKII activity assay.
SGC-CAMKK2-1, a chemical probe, is the selective, inhibitor for calcium/calmodulin-dependent protein kinase kinase 2 (CAMKK2) with an IC50 of 30 nM. SGC-CAMKK2-1 inhibits AMPK phosphorylation in cell C4-2 with an IC50 of 1.6 μM .
Bacoside A is an orally active, blood-brain barrier-permeable triterpenoid saponin that modulates the activities of ATPases, AChE, CaMK2A and iNOS. Derived from Bacopa monniera. Bacoside A exerts significant antioxidant, anti-inflammatory and anti-apoptotic effects by maintaining ion balance, scavenging reactive oxygen species, stabilizing cell membranes, and regulating the expression of NF-κB and apoptosis-related proteins. Bacoside A counteracts morphine-induced reductions in Na +/K +-ATPase, Ca 2+-ATPase and Mg 2+-ATPase activities, increases mitochondrial membrane potential, and decreases intracellular reactive oxygen species levels. Bacoside A specifically binds to calcium/calmodulin-dependent protein kinase IIA to trigger endoplasmic reticulum calcium release. Bacoside A exhibits non-apoptotic cytotoxicity against glioblastoma cells while protecting normal nerve cells from stress-induced damage. Bacoside A is applicable to the research of Parkinson's disease and glioblastoma multiforme .
Neurogranin (48-76), mouse is a peptide corresponding to residues 48-76 of Neurogranin. Neurogranin, a calmodulin-binding protein, is exclusively expressed in the post-synapse, and mediates NMDAR driven synaptic plasticity by regulating the calcium-calmodulin (Ca 2+-CaM) pathway .
CaMKI (299-320) refers to a peptide consisting of residues 299-320 of Calcium/calmodulin-dependent protein kinase I (CaMKI). CaMKI (299-320), as a protein kinase, has a high affinity interaction with Ca 2+-CAM (Kd≤1 nM≤1 nM), which can phosphorylate specific substrate proteins, thereby regulating their activity. CaMKI (299-320) contains the CAM-binding domain and the self-inhibition domain, and CaMKI (299-320) can be used to study cell physiological processes, including cell proliferation, differentiation, and apoptosis .
Ferroptosis/apoptosis inducer-3 (Compound 34) is a Ferroptosis and Apoptosis inducer. Ferroptosis/apoptosis inducer-3 induces both Ferroptosis and Apoptosis by causing G2/M phase cell cycle arrest, disrupting mitochondrial membrane potentials, promoting lipid peroxidation, and increasing the levels of Ca 2+ and Fe 2+ through the activation of calcium/calmodulin signaling. Ferraplasm/apoptosis inducer-3 shows anticancer effects against cervical cancer, adenocarcinoma, breast cancer, colon cancer, and colorectal carcinoma .
Neuropeptide DF2 is a DRNFLRFamide neuropeptide orignally isolated from crayfish. which enhances transmitter release and stimulates the amplitude of excitatory post-synaptic potentials (EPSP) through the calcium/calmodulin-dependent protein kinase .
[Ala286]-Calmodulin-Dependent Protein Kinase II (281-302) is a modified fragment of calcium/calmodulin-dependent protein kinase II that contains the active domain of CaMKII and has an alanine substitution at position 286. [Ala286]-Calmodulin-Dependent Protein Kinase II (281-302) can be used to develop more potent CaMKII inhibitors .
Ziptide is a substrate for MAPK activated protein kinase 2 (MAPKAPK2, Km = 5 μM), MAPKAPK3 (Km = 30 μM), PARK (Km = 40 μM), checkpoint kinase 1 (Chk1, Km = 5 μM), AMP-activated protein kinase (AMPK, Km = 75 μM), and calcium/calmodulin-dependent protein kinase II (CamKII, Km = 300 μM) .
Junicedric acid is a diterpenoid compound that can be isolated from the resin of the Araucaria araucana tree. Junicedric acid exerts neuroprotective activity by increasing intracellular calcium levels in hippocampal neurons, activating PKC and calcium/calmodulin-dependent protein kinase (CaMKII), and preventing Amyloid-β oligomer-induced synaptic protein loss, apoptosis, and long-term potentiation (LTP) inhibition. Junicedric acid can be used in the study of the pathological mechanisms of neurodegenerative diseases such as Alzheimer's disease .
NPC-15437 is a selective PKC inhibitor with an IC50 of 19 µM. NPC-15437 competitively inhibits phorbol ester- (Ki of 5 µM) and phosphatidylserine-induced (Ki of 12 µM) PKC activity. NPC-15437 does not inhibits cAMP-dependent or calcium/calmodulin-dependent protein kinases. NPC-15437 augments TRAIL-induced cell death in non-small cell lung cancer and medulloblastoma cells. NPC-15437 can be used for the research of non-small cell lung cancer, medulloblastoma, and neurological disease .
TAT-CN21 is a potent CaMKII inhibitor with an IC50 of 77.2 nM. TAT-CN21 inhibits both calcium/calmodulin-dependent and autonomously activated CaMKII, blocks glutamate-induced translocation of CaMK IIα, and reverses the enhanced phosphorylation of CaMKII at Thr286 following excitotoxic injury. TAT-CN21 shows application potential in studies related to ischemic stroke by reducing neuronal excitotoxicity and exacerbating pre-existing long-term neuronal death prior to injury. TAT-CN21 improves definitive behaviors in rats with residual nerve injury without altering indicators such as mechanical/thermal hyperalgesia or spatial memory. TAT-CN21 can also be used in studies related to neuropathic pain .
Autocamtide 2 is a highly selective peptide substrate of calcium/calmodulin-dependent protein kinase II (CaMKII). It can be used in the CaMKII activity assay.
Neurogranin (48-76), mouse is a peptide corresponding to residues 48-76 of Neurogranin. Neurogranin, a calmodulin-binding protein, is exclusively expressed in the post-synapse, and mediates NMDAR driven synaptic plasticity by regulating the calcium-calmodulin (Ca 2+-CaM) pathway .
CaMKI (299-320) refers to a peptide consisting of residues 299-320 of Calcium/calmodulin-dependent protein kinase I (CaMKI). CaMKI (299-320), as a protein kinase, has a high affinity interaction with Ca 2+-CAM (Kd≤1 nM≤1 nM), which can phosphorylate specific substrate proteins, thereby regulating their activity. CaMKI (299-320) contains the CAM-binding domain and the self-inhibition domain, and CaMKI (299-320) can be used to study cell physiological processes, including cell proliferation, differentiation, and apoptosis .
Neuropeptide DF2 is a DRNFLRFamide neuropeptide orignally isolated from crayfish. which enhances transmitter release and stimulates the amplitude of excitatory post-synaptic potentials (EPSP) through the calcium/calmodulin-dependent protein kinase .
[Ala286]-Calmodulin-Dependent Protein Kinase II (281-302) is a modified fragment of calcium/calmodulin-dependent protein kinase II that contains the active domain of CaMKII and has an alanine substitution at position 286. [Ala286]-Calmodulin-Dependent Protein Kinase II (281-302) can be used to develop more potent CaMKII inhibitors .
Ziptide is a substrate for MAPK activated protein kinase 2 (MAPKAPK2, Km = 5 μM), MAPKAPK3 (Km = 30 μM), PARK (Km = 40 μM), checkpoint kinase 1 (Chk1, Km = 5 μM), AMP-activated protein kinase (AMPK, Km = 75 μM), and calcium/calmodulin-dependent protein kinase II (CamKII, Km = 300 μM) .
Bacoside A is an orally active, blood-brain barrier-permeable triterpenoid saponin that modulates the activities of ATPases, AChE, CaMK2A and iNOS. Derived from Bacopa monniera. Bacoside A exerts significant antioxidant, anti-inflammatory and anti-apoptotic effects by maintaining ion balance, scavenging reactive oxygen species, stabilizing cell membranes, and regulating the expression of NF-κB and apoptosis-related proteins. Bacoside A counteracts morphine-induced reductions in Na +/K +-ATPase, Ca 2+-ATPase and Mg 2+-ATPase activities, increases mitochondrial membrane potential, and decreases intracellular reactive oxygen species levels. Bacoside A specifically binds to calcium/calmodulin-dependent protein kinase IIA to trigger endoplasmic reticulum calcium release. Bacoside A exhibits non-apoptotic cytotoxicity against glioblastoma cells while protecting normal nerve cells from stress-induced damage. Bacoside A is applicable to the research of Parkinson's disease and glioblastoma multiforme .
Junicedric acid is a diterpenoid compound that can be isolated from the resin of the Araucaria araucana tree. Junicedric acid exerts neuroprotective activity by increasing intracellular calcium levels in hippocampal neurons, activating PKC and calcium/calmodulin-dependent protein kinase (CaMKII), and preventing Amyloid-β oligomer-induced synaptic protein loss, apoptosis, and long-term potentiation (LTP) inhibition. Junicedric acid can be used in the study of the pathological mechanisms of neurodegenerative diseases such as Alzheimer's disease .
CAMK1; calcium/calmodulin-dependent protein kinase I; calcium/calmodulin-dependent protein kinase type 1; CaMKI; caM-KI; caMKI-alpha; caM kinase I alpha; CAMKI; MGC120317; MGC120318;
CAMK1 alpha/CAMK1 Protein, Human (sf9, His, GST) is the recombinant human-derived CAMK1 alpha, expressed by Sf9 insect cells , with His, GST labeled tag.
CAMKIV/CAMK4 protein, a calcium/calmodulin-dependent kinase, regulates transcription activators (CREB1, MEF2D, JUN, and RORA) in immune response, inflammation, and memory consolidation. It controls thymocyte selection, TCR signaling, osteoclast and dendritic cell survival, and memory consolidation in hippocampal neurons. It activates MAP kinases, ELK1, ATF2, and potentially participates in spermatogenesis. CAMKIV/CAMK4 Protein, Mouse (sf9) is the recombinant mouse-derived CAMKIV/CAMK4 protein, expressed by Sf9 insect cells, with tag free.
CaMKII α/CAMK2A protein functions autonomously upon Ca(2+)/calmodulin binding and autophosphorylation. CaMKII alpha/CAMK2A Protein, Human (sf9, GST) is the recombinant human-derived CaMKII alpha/CAMK2A protein, expressed by Sf9 insect cells , with N-GST labeled tag.
The CAMKK1 protein is a calcium/calmodulin-dependent kinase and a key player in calcium-triggered signaling cascades involved in various cellular processes. It phosphorylates multiple members of the CAMK1 family, including CAMK1, CAMK1D, CAMK1G, and CAMK4. CAMKK1 Protein, Human (sf9) is the recombinant human-derived CAMKK1 protein, expressed by sf9 insect cells , with tag free.
The CAMKK1 protein is a calcium/calmodulin-dependent kinase and a key player in calcium-triggered signaling cascades involved in various cellular processes. It phosphorylates multiple members of the CAMK1 family, including CAMK1, CAMK1D, CAMK1G, and CAMK4. CAMKK1 Protein, Human (sf9, His) is the recombinant human-derived CAMKK1 protein, expressed by sf9 insect cells , with N-6*His labeled tag.
CaMKII beta/CAMK2B is a calcium/calmodulin-dependent protein kinase that functions autonomously upon Ca(2+)/calmodulin binding and autophosphorylation. It plays an important role in dendritic spine and synapse formation, neuronal plasticity, and the regulation of skeletal muscle sarcoplasmic reticulum Ca(2+) transport. CaMKII beta/CAMK2B Protein, Human (sf9, His-GST) is the recombinant human-derived CaMKII beta/CAMK2B protein, expressed by Sf9 insect cells , with N-His, N-GST labeled tag.
CAMKI alpha/CAMK1 Protein, Human, a recombinant human CAMK1 produced in HEK293 cells. CAMK1 is involved in multiple cell functions, including calmodulin binding, ATP binding, signal transduction, development and cell differentiation.
CAMKI delta/CAMK1D Protein, Human (GST), a recombinant human CAMK1D expressed in E. coli, has a GST tag at the N-terminus. CAMK1D acts as a key modulator of tumor-intrinsic immune resistance.
The CAMK1G protein is a calcium/calmodulin-dependent protein kinase and a key component of the proposed calcium-triggered signaling cascade. This kinase exhibits the ability to phosphorylate the transcription factor CREB1 in vitro, suggesting its involvement in regulating cellular processes related to gene expression and transcriptional control. CAMKI gamma/CAMK1G Protein, Human (sf9, His, GST) is the recombinant human-derived CAMKI gamma/CAMK1G protein, expressed by Sf9 insect cells , with His, GST labeled tag.
CAMKI alpha/CAMK1 Protein, Human (HEK293, His), a recombinant human CAMK1 produced in HEK293 cells, has a His tag at the N-terminus. CAMK1 is involved in multiple cell functions, including calmodulin binding, ATP binding, signal transduction, development and cell differentiation.
PDE1B protein, a cyclic nucleotide phosphodiesterase, displays dual specificity for cAMP and cGMP, essential second messengers in physiological processes. Preferring cGMP as a substrate, PDE1B fine-tunes cellular responses to cyclic nucleotide signaling. Its dual specificity underscores its regulatory role in diverse physiological pathways. PDE1B Protein, Human (sf9, His-GST) is the recombinant human-derived PDE1B protein, expressed by Sf9 insect cells , with N-His, N-GST labeled tag.
EEF2K, a threonine kinase, critically regulates protein synthesis by modulating peptide chain elongation. Activation by upstream kinases like AMPK or TRPM7 leads to phosphorylation of EEF2, inhibiting its binding to ribosomes and decelerating protein synthesis. EEF2K's intricate control underscores its pivotal role in modulating cellular processes related to protein translation. EEF2K Protein, Human (GST) is the recombinant human-derived EEF2K protein, expressed by E. coli , with N-GST labeled tag.
CAMK2D protein regulates calcium levels and excitation-contraction coupling in the heart. CaMKII delta/CAMK2D Protein, Human (sf9, GST) is the recombinant human-derived CAMK2D protein, expressed by sf9 insect cells , with N-GST labeled tag.
The PNCK protein is a calcium/calmodulin-dependent protein kinase and a key component of the calcium-triggered signaling cascade. In vitro, PNCK exhibits the ability to phosphorylate CREB1 and SYN1/synapsin I, emphasizing its involvement in regulating cellular processes associated with these substrates. PNCK Protein, Human (sf9, His, GST) is the recombinant human-derived PNCK protein, expressed by sf9 insect cells , with N-8*His, N-GST labeled tag.
Alpha CaMKII; calciumcalmodulin dependent protein kinase II; calcium/calmodulin dependent protein kinase II alpha B subunit; calcium/calmodulin dependent protein kinase type II alpha chain; calcium/calmodulin-dependent protein kinase (CaM kinase) II alpha; calcium/calmodulin-dependent protein kinase II alpha; calcium/calmodulin-dependent protein kinase II-alpha; calcium/calmodulin-dependent protein kinase type II subunit alpha; calcium/calmodulin-dependent protein kinase type IIA; CaM kinase II alpha chain
WB, IHC-P, IP, ICC/IF, IF-Tissue
Human, Mouse, Rat
Phospho-CaMKII (Thr286) Antibody (YA9806) is a Mouse-derived and non-conjugated IgG1 Monoclonal antibody, targeting to Phospho-CaMKII (Thr286).
calcium/calmodulin dependent protein kinase II alpha antibody;
calcium/calmodulin dependent protein kinase II beta antibody;
calcium/calmodulin dependent protein kinase II delta antibody;
calcium/calmodulin dependent protein kinase II gamma antibody;
calcium/calmodulin-dependent protein kinase type II subunit alpha antibody;
CaM kinase II alpha antibody;
CaM kinase II antibody;
CaM kinase II beta antibody;
CaM kinase II delta antibody;
CaM kinase II gamma antibody;
CaM kinase II subunit alpha antibody;
CaMK-II subunit alpha antibody;
CAMK2 antibody;
Camk2a antibody;
CAMK2B antibody;
CAMK2D antibody;
CAMK2G antibody;
CAMKA antibody;
KCC2A_HUMAN antibody;
CaMKⅡ
WB, IHC-P, IHC-F, ICC/IF, FC, IF-Tissue
Human, Mouse, Rat, Monkey, Pig
CaMKII Antibody (YA4627) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to CaMKII.
Alpha CaMKII; calciumcalmodulin dependent protein kinase II; calcium/calmodulin dependent protein kinase II alpha B subunit; calcium/calmodulin dependent protein kinase type II alpha chain; calcium/calmodulin-dependent protein kinase (CaM kinase) II alpha; calcium/calmodulin-dependent protein kinase II alpha; calcium/calmodulin-dependent protein kinase II-alpha; calcium/calmodulin-dependent protein kinase type II subunit alpha; calcium/calmodulin-dependent protein kinase type IIA; CaM kinase II alpha chain; CaM kinase II alpha subunit; CaM kinase II subunit alpha; CaMK II alpha subunit; CaMK-II subunit alpha; Camk2a; CAMKA; CaMKII; CaMKIINalpha; EC2.7.11.17; KCC2A_HUMAN; KIAA0968; MGC123320; MGC139375; MGC155201; mKIAA0968; PK2CDD; PKCCD; R74975; zgc:112538; zgc:123320;
WB, IP, FC, IHC-P
Human, Mouse, Rat
CaMKII alpha Antibody (YA569) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to CaMKII alpha.
Brain Ca; 2; calmodulin dependent protein kinase type 4; Brain Ca; 2; calmodulin dependent protein kinase type IV; Brain Ca -calmodulin dependent protein kinase type IV; calcium / calmodulin dependent protein kinase type 4 catalytic chain; calcium / calmodulin dependent protein kinase type IV catalytic chain; calcium/calmodulin dependent protein kinase IV; calcium/calmodulin dependent protein kinase type IV; calcium/calmodulin-dependent protein kinase type IV; CAM kinase 4; CAM kinase GR; CAM kinase IV; CAM kinase-GR; CaMK 4; CAMK GR; CaMK IV; CaMK4; CaMKGR; IV; KCC4_HUMAN; MGC36771.
WB
Human
CaMKIV Antibody (YA5229) is a Mouse-derived and non-conjugated monoclonal antibody, targeting to CaMKIV.
CAMK2B; CAM2; CAMK2; CAMKB; calcium/calmodulin-dependent protein kinase type II subunit beta; CaM kinase II subunit beta; CaMK-II subunit beta; CAMK2G; CAMK; CAMK-II; CAMKG; calcium/calmodulin-dependent protein kinase type II subunit gamma
WB
Mouse
CaMKII beta Antibody (YA2344) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to CaMKII beta.
CAMK2B; CAM2; CAMK2; CAMKB; calcium/calmodulin-dependent protein kinase type II subunit beta; CaM kinase II subunit beta; CaMK-II subunit beta; CAMK2G; CAMK; CAMK-II; CAMKG; calcium/calmodulin-dependent protein kinase type II subunit gamma;
IHC-P, ICC/IF
Human, Rat, Mouse
Phospho-CaMKIIβ/γ/δ Antibody (YA5758) is a Mouse-derived and non-conjugated monoclonal antibody, targeting to Phospho-CaMKIIβ/γ/δ.
CAMK2B; CAM2; CAMK2; CAMKB; calcium/calmodulin-dependent protein kinase type II subunit beta; CaM kinase II subunit beta; CaMK-II subunit beta; CAMK2G; CAMK; CAMK-II; CAMKG; calcium/calmodulin-dependent protein kinase type II subunit gamma
WB
Human, Mouse, Rat
Phospho-CaMKII (Thr286) Antibody (YA9815) is a Rabbit-derived and non-conjugated IgG Recombinant, Monoclonal antibody, targeting to Phospho-CaMKII (Thr286).
CAMK2B; CAM2; CAMK2; CAMKB; calcium/calmodulin-dependent protein kinase type II subunit beta; CaM kinase II subunit beta; CaMK-II subunit beta; CAMK2G; CAMK; CAMK-II; CAMKG; calcium/calmodulin-dependent protein kinase type II subunit gamma
WB
Mouse
CaMKII beta Antibody (YA2344) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to CaMKII beta.
Western blot analysis of extracts from THP-1(lane 2(20μg), Jurkat (lane 3(20μg) and NIH3T3(lane 4(20μg) using FOXO1A (HY-P80132) Rabbit mAb. Proteins were transferred
to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was
used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
Western blot analysis of extracts from THP-1(lane 2(20μg), Jurkat (lane 3(20μg) and NIH3T3(lane 4(20μg) using FOXO1A (HY-P80132) Rabbit mAb. Proteins were transferred
to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was
used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
Western blot analysis of extracts from THP-1(lane 2(20μg), Jurkat (lane 3(20μg) and NIH3T3(lane 4(20μg) using FOXO1A (HY-P80132) Rabbit mAb. Proteins were transferred
to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was
used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
Western blot analysis of extracts from THP-1(lane 2(20μg), Jurkat (lane 3(20μg) and NIH3T3(lane 4(20μg) using FOXO1A (HY-P80132) Rabbit mAb. Proteins were transferred
to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was
MedchemExpress Validation 03
Western blot analysis of extracts from THP-1(lane 2(20μg), Jurkat (lane 3(20μg) and NIH3T3(lane 4(20μg) using FOXO1A (HY-P80132) Rabbit mAb. Proteins were transferred
MedchemExpress Validation 04
Western blot analysis of extracts from THP-1(lane 2(20μg), Jurkat (lane 3(20μg) and NIH3T3(lane 4(20μg) using FOXO1A (HY-P80132) Rabbit mAb. Proteins were transferred
to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was
used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
MedchemExpress Validation
Western blot analysis of extracts from THP-1(lane 2(20μg), Jurkat (lane 3(20μg) and NIH3T3(lane 4(20μg) using FOXO1A (HY-P80132) Rabbit mAb. Proteins were transferred
to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was
used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
Western blot analysis of extracts from THP-1(lane 2(20μg), Jurkat (lane 3(20μg) and NIH3T3(lane 4(20μg) using FOXO1A (HY-P80132) Rabbit mAb. Proteins were transferred
to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was
used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
MedchemExpress Validation
Western blot analysis of extracts from THP-1(lane 2(20μg), Jurkat (lane 3(20μg) and NIH3T3(lane 4(20μg) using FOXO1A (HY-P80132) Rabbit mAb. Proteins were transferred
to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was
used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
MedchemExpress Validation
Western blot analysis of extracts from THP-1(lane 2(20μg), Jurkat (lane 3(20μg) and NIH3T3(lane 4(20μg) using FOXO1A (HY-P80132) Rabbit mAb. Proteins were transferred
to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was
used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
MedchemExpress Validation
Western blot analysis of extracts from THP-1(lane 2(20μg), Jurkat (lane 3(20μg) and NIH3T3(lane 4(20μg) using FOXO1A (HY-P80132) Rabbit mAb. Proteins were transferred
to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was
used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
MedchemExpress Validation
Western blot analysis of extracts from THP-1(lane 2(20μg), Jurkat (lane 3(20μg) and NIH3T3(lane 4(20μg) using FOXO1A (HY-P80132) Rabbit mAb. Proteins were transferred
to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was
used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
MedchemExpress Validation
Western blot analysis of extracts from THP-1(lane 2(20μg), Jurkat (lane 3(20μg) and NIH3T3(lane 4(20μg) using FOXO1A (HY-P80132) Rabbit mAb. Proteins were transferred
to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was
used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
MedchemExpress Validation
Western blot analysis of extracts from THP-1(lane 2(20μg), Jurkat (lane 3(20μg) and NIH3T3(lane 4(20μg) using FOXO1A (HY-P80132) Rabbit mAb. Proteins were transferred
to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was
used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
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