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  2. Calmodulin-Dependent Protein Kinase II 281-309

Calmodulin-Dependent Protein Kinase II 281-309 

Cat. No.: HY-P1874
Handling Instructions

Calmodulin-Dependent Protein Kinase II (281-309) is a peptide of calcium/calmodulin-dependent protein kinase II (CaM-kinase II).

For research use only. We do not sell to patients.

Custom Peptide Synthesis

Calmodulin-Dependent Protein Kinase II 281-309 Chemical Structure

Calmodulin-Dependent Protein Kinase II 281-309 Chemical Structure

CAS No. : 116826-37-0

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Description

Calmodulin-Dependent Protein Kinase II (281-309) is a peptide of calcium/calmodulin-dependent protein kinase II (CaM-kinase II)[1].

Molecular Weight

3374.06

Formula

C₁₄₆H₂₅₄N₄₆O₃₉S₃

CAS No.

116826-37-0

Sequence

{Met}{His}{Arg}{Gln}{Glu}{Thr}{Val}{Asp}{Cys}{Leu}{Lys}{Lys}{Phe}{Asn}{Ala}{Arg}{Arg}{Lys}{Leu}{Lys}{Gly}{Ala}{Ile}{Leu}{Thr}{Thr}{Met}{Leu}{Ala}

Sequence Shortening

MHRQETVDCLKKFNARRKLKGAILTTMLA

Shipping

Room temperature in continental US; may vary elsewhere

Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

Solvent & Solubility
In Vitro: 

H2O

Peptide Solubility and Storage Guidelines:

1.  Calculate the length of the peptide.

2.  Calculate the overall charge of the entire peptide according to the following table:

  Contents Assign value
Acidic amino acid Asp (D), Glu (E), and the C-terminal -COOH. -1
Basic amino acid Arg (R), Lys (K), His (H), and the N-terminal -NH2 +1
Neutral amino acid Gly (G), Ala (A), Leu (L), Ile (I), Val (V), Cys (C), Met (M), Thr (T), Ser (S), Phe (F), Tyr (Y), Trp (W), Pro (P), Asn (N), Gln (Q) 0

3.  Recommended solution:

Overall charge of peptide Details
Negative (<0) 1.  Try to dissolve the peptide in water first.
2.  If water fails, add NH4OH (<50 μL).
3.  If the peptide still does not dissolve, add DMSO (50-100 μL) to solubilize the peptide.
Positive (>0) 1.  Try to dissolve the peptide in water first.
2.  If water fails, try dissolving the peptide in a 10%-30% acetic acid solution.
3.  If the peptide still does not dissolve, try dissolving the peptide in a small amount of DMSO.
Zero (=0) 1.  Try to dissolve the peptide in organic solvent (acetonitrile, methanol, etc.) first.
2.  For very hydrophobic peptides, try dissolving the peptide in a small amount of DMSO, and then dilute the solution with water to the desired concentration.
References
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  • Molarity Calculator

  • Dilution Calculator

The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass   Concentration   Volume   Molecular Weight *
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The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

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Product Name:
Calmodulin-Dependent Protein Kinase II 281-309
Cat. No.:
HY-P1874
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