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CAMKI delta/CAMK1D Protein, Human (GST)

CAMKI delta/CAMK1D Protein, Human (GST), a recombinant human CAMK1D expressed in E. coli, has a GST tag at the N-terminus. CAMK1D acts as a key modulator of tumor-intrinsic immune resistance.

For research use only. We do not sell to patients.
  • Species: Human
  • Source: E. coli
  • Storage:
    Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
  • CAMKI delta/CAMK1D Protein, Human (GST) Product Image
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[email protected] [email protected]
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  • Biological Activity
  • Technical Parameters
  • Product Properties
  • Documentation
  • References
  • Help & FAQs
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Biological Activity

Description

CAMKI delta/CAMK1D Protein, Human (GST), a recombinant human CAMK1D expressed in E. coli, has a GST tag at the N-terminus. CAMK1D acts as a key modulator of tumor-intrinsic immune resistance[1].

Background

Calcium/calmodulin-dependent protein kinase 1D (CAMK1D) is coexpressed with PD-L1 in anti-PD-L1/PD-1 treatment-refractory cancer types and correlated with poor prognosis in these tumors. CAMK1D is activated by CTL through Fas-receptor stimulation, which leads to CAMK1D binding to and phosphorylating caspase-3, -6, and -7, inhibiting their activation and function[1].

Technical Parameters

  • Species Human
  • Source E. coli
  • Tag N-GST
  • Accession

    Q8IU85-1 (M1-K385)

  • Gene ID
    57118  [NCBI]
  • Molecular Construction
    • N-term
    • GST
    • CAMK1D (M1-K385)
      Accession # Q8IU85-1
    • C-term
  • Protein Length

    Full Length of Isoform-1

  • Synonyms

    CAMK1; CaM Kinase I Alpha; Calcium/Calmodulin Dependent Protein Kinase I; CaM-KI; CaMKI-Alpha; Calcium/Calmodulin-Dependent Protein Kinase; CaMKI; CaM Kinase I; Calcium/Calmodulin-Dependent Protein Kinase Type 1

  • AA Sequence

    MARENGESSSSWKKQAEDIKKIFEFKETLGTGAFSEVVLAEEKATGKLFAVKCIPKKALKGKESSIENEIAVLRKIKHENIVALEDIYESPNHLYLVMQLVSGGELFDRIVEKGFYTEKDASTLIRQVLDAVYYLHRMGIVHRDLKPENLLYYSQDEESKIMISDFGLSKMEGKGDVMSTACGTPGYVAPEVLAQKPYSKAVDCWSIGVIAYILLCGYPPFYDENDSKLFEQILKAEYEFDSPYWDDISDSAKDFIRNLMEKDPNKRYTCEQAARHPWIAGDTALNKNIHESVSAQIRKNFAKSKWRQAFNATAVVRHMRKLHLGSSLDSSNASVSSSLSLASQKDCLAPSTLCSFISSSSGVSGVGAERRPRPTTVTAVHSGSK

  • Predicted Molecular Mass

    69.2 kDa

  • Molecular Weight

    Approximately 60-70 kDa, based on SDS-PAGE under reducing conditions.

  • Purity

    ≥ 90%, as determined by reducing SDS-PAGE.

Product Properties

Appearance

Solution.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

Please use rapid thawing with running water to thaw the protein.

Storage & Stability

Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping

Shipping with dry ice.

References

[1]. Valentina Volpin, et al. CAMK1D Triggers Immune Resistance of Human Tumor Cells Refractory to Anti-PD-L1 Treatment. Cancer Immunol Res. 2020 Sep;8(9):1163-1179. [Content Brief]

Calculators

Reconstitution Calculator

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

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Dilution Calculator

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

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The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50) Biological Activity (ED50)
106 ÷
ng/mL
MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
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