2. Cell Cycle/DNA Damage Epigenetics
  3. Sirtuin
  4. SIRT1-IN-3

SIRT1-IN-3 (compound 3j) is a potent and selective SIRT1 inhibitor, with an IC50 of 4.2 μM.

For research use only. We do not sell to patients.

SIRT1-IN-3 Chemical Structure

SIRT1-IN-3 Chemical Structure

CAS No. : 2470969-91-4

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Based on 1 publication(s) in Google Scholar

SIRT1-IN-3 Related Antibodies

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Description

SIRT1-IN-3 (compound 3j) is a potent and selective SIRT1 inhibitor, with an IC50 of 4.2 μM[1].

IC50 & Target

SIRT1

4.2 μM (IC50)

SIRT2

38 μM (IC50)

In Vitro

SIRT1-IN-3 (compound 3j) (0-100 μM, 48 h) inhibits the proliferation of human cancer cell lines including K562, HCT-116, H460, HepG2, A549, and MCF-7, and shows low cytotoxic on 293T and HUVEC[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

SIRT1-IN-3 Related Antibodies

Cell Cytotoxicity Assay

Cell Line: Human cancer cell lines (K562, HCT-116, H460, HepG2, A549, HT-29, MCF-7) and normal cell lines (293T, HUVEC)[1]
Concentration: 0, 0.01, 0.1, 1, 10, 100 μM
Incubation Time: 48 h
Result: Inhibited the proliferation of Human cancer cell lines including K562, HCT-116, H460, HepG2, A549, and MCF-7, with IC50 values of 47, 41, 66, 93, 52, and 64 μM, respectively. And showed low cytotoxic on 293T and HUVEC, with an IC50 values of 49 and 45 μM, respectively.
Molecular Weight

295.18

Formula

C13H15BrN2O
CAS No.
Appearance

Solid

Color

White to off-white

SMILES

CC(C1=C(NC2=CC=C(C=C21)Br)CC(N)=O)C
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, sealed storage, away from moisture and light

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

Solvent & Solubility
In Vitro: 

DMSO : 100 mg/mL (338.78 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 3.3878 mL 16.9388 mL 33.8776 mL
5 mM 0.6776 mL 3.3878 mL 6.7755 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 2.5 mg/mL (8.47 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
% 
+
%
Tween-80 +
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 3.3878 mL 16.9388 mL 33.8776 mL 84.6941 mL
5 mM 0.6776 mL 3.3878 mL 6.7755 mL 16.9388 mL
10 mM 0.3388 mL 1.6939 mL 3.3878 mL 8.4694 mL
15 mM 0.2259 mL 1.1293 mL 2.2585 mL 5.6463 mL
20 mM 0.1694 mL 0.8469 mL 1.6939 mL 4.2347 mL
25 mM 0.1355 mL 0.6776 mL 1.3551 mL 3.3878 mL
30 mM 0.1129 mL 0.5646 mL 1.1293 mL 2.8231 mL
40 mM 0.0847 mL 0.4235 mL 0.8469 mL 2.1174 mL
50 mM 0.0678 mL 0.3388 mL 0.6776 mL 1.6939 mL
60 mM 0.0565 mL 0.2823 mL 0.5646 mL 1.4116 mL
80 mM 0.0423 mL 0.2117 mL 0.4235 mL 1.0587 mL
100 mM 0.0339 mL 0.1694 mL 0.3388 mL 0.8469 mL
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SIRT1-IN-3 Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

SIRT1-IN-32470969-91-4SirtuinIndolesCancerCytotoxicityInhibitorinhibitorinhibit

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