2. Cell Cycle/DNA Damage
  3. Deubiquitinase
  4. T-10531

T-10531 is a selective USP25/USP28 inhibitor. T-10531 exhibits an IC50 of 0.03 μM and a Kd of 0.2 μM against human USP25, as well as an IC50 of 0.12 μM and a Kd of 0.06 μM against human USP28. T-10531 inhibits USP25/USP28 activity and induces the degradation of USP25 via the proteasomal pathway, without inhibiting other deubiquitinases. T-10531 can be used in the research of squamous cell carcinoma, colorectal cancer, gastric cancer, triple-negative breast cancer and pancreatic cancer.

For research use only. We do not sell to patients.

T-10531

T-10531 Chemical Structure

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T-10531 Related Antibodies

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USP1 USP2 USP4 USP7 USP8 USP10 USP21 USP30 UCHL1 USP11 USP15 USP28 USP25

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Description

T-10531 is a selective USP25/USP28 inhibitor. T-10531 exhibits an IC50 of 0.03 μM and a Kd of 0.2 μM against human USP25, as well as an IC50 of 0.12 μM and a Kd of 0.06 μM against human USP28. T-10531 inhibits USP25/USP28 activity and induces the degradation of USP25 via the proteasomal pathway, without inhibiting other deubiquitinases. T-10531 can be used in the research of squamous cell carcinoma, colorectal cancer, gastric cancer, triple-negative breast cancer and pancreatic cancer[1].

In Vitro

T-10531 (compound 42) potently inhibits recombinant USP25 with an IC50 of 0.03 μM and recombinant USP28 with an IC50 of 0.12 μM in a Ub-Rho110 cleavage assay[1].
T-10531 binds to recombinant USP25 with a Kd of 0.2 μM and to recombinant USP28 with a Kd of 0.06 μM in an ITC assay[1].
T-10531 (72 h) exhibits no toxicity to HepG2 hepatocellular carcinoma cells[1].
T-10531 directly binds and stabilizes recombinant USP25, causing a 7.0 °C thermal shift, and recombinant USP28, causing a 3.8 °C thermal shift in a DSF assay[1].
T-10531 (0.1-50 μM; 4 h, 10 μM; 10-240 min) dose-dependently inhibits USP25 and USP28 activity in live HeLa cells without affecting other DUBs, and induces proteasome-dependent degradation of USP25 in a time- and dose-dependent manner[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

T-10531 Related Antibodies

Western Blot Analysis[1]

Cell Line: HeLa cells
Concentration: 0.1, 0.5, 1, 5, 10, 50 μM
Incubation Time: 4 h
Result: Dose-dependently reduced USP25 protein level and USP25/USP28 activity-based probe labeling.

Western Blot Analysis[1]

Cell Line: HeLa cells
Concentration: 10 μM
Incubation Time: 10, 30, 60, 90, 120, 240 min
Result: Reduced USP25 protein level in a time-dependent manner.

Western Blot Analysis[1]

Cell Line: HeLa cells
Concentration: 0.1, 0.5, 1, 5, 10 μM (with 750 nM MG132 (HY-13259))
Incubation Time: 4 h
Result: Reduced USP25 protein level, which is rescued by the proteasome inhibitor MG132.
Reduced USP25 protein level regardless of USP28 knockdown.
Molecular Weight

665.88

Formula

C26H22BrClF4N2O5S
SMILES

O=C(NC1=CC(F)=C(F)C(COC2=CC(F)=C(Br)C=C2CN3C[C@@H](O)CC3)=C1F)C4=CC=C(S(=O)(C)=O)C=C4Cl
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Room temperature in continental US; may vary elsewhere.
Storage

Please store the product under the recommended conditions in the Certificate of Analysis.
Purity & Documentation
References
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T-10531 Related Classifications

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Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

T-10531T10531T 10531DeubiquitinaseDUBssquamous cell carcinomagastric cancerUSP25colorectal cancertriple-negative breast cancerpancreatic cancerHEK293T cellsHeLa cellsHepG2 hepatocellular carcinoma cellsUSP28Inhibitorinhibitorinhibit

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