Isochlorogenic acid A (Synonyms: 3,5-Dicaffeoylquinic acid; 3,5-CQA)

Name: Isochlorogenic acid A
Brand: MedChemExpress
SKU: HY-N0056
Rating: 5.0 (13 reviews)

Cat. No.: HY-N0056 Purity: 99.53%: Data Sheet
COA

SDS
Handling Instructions
FAQs
Technical Support

Isochlorogenic acid A (3,5-Dicaffeoylquinic acid) is a natural phenolic acid with anti-mutagenicity, anti-HBV, anti-HIV, anti-oxidant, anti-bacterial, and anti-inflammatoryy activities.

For research use only. We do not sell to patients.

CAS No. : 2450-53-5

Size	Stock	Quantity

Free Sample (0.1 - 0.2 mg)	Apply Now
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	In-stock	Estimated Time of Arrival: December 31
Solid
5 mg	In-stock	Estimated Time of Arrival: December 31
10 mg	In-stock	Estimated Time of Arrival: December 31
25 mg	In-stock	Estimated Time of Arrival: December 31
50 mg	In-stock	Estimated Time of Arrival: December 31
100 mg	In-stock	Estimated Time of Arrival: December 31
200 mg	Get quote
500 mg	Get quote

or Bulk Inquiry

* Please select Quantity before adding items.

This product is a controlled substance and not for sale in your territory.

Customer Review

Based on 13 publication(s) in Google Scholar

Other Forms of Isochlorogenic acid A:

Isochlorogenic acid A (Standard) Get quote

13 Publications Citing Use of MCE Isochlorogenic acid A

Cell Proliferation/Viability Assay

: Isochlorogenic acid A purchased from MedChemExpress. Usage Cited in: Antiviral Res. 2025 Dec 4:245:106323. [Abstract]; Cytotoxicity assay of Isochlorogenic acid A (ICAA) on HepAD38 and HepG2 cells. Cells were incubated with different concentrations (up to 1000 μM) of ICAA for 6 days, and the toxicity was assessed using a cell viability assay (Presto Blue).

: Isochlorogenic acid A purchased from MedChemExpress. Usage Cited in: Antiviral Res. 2025 Dec 4:245:106323. [Abstract]; Isochlorogenic acid A (ICAA: 200 μM). Representative images from Western blots showing the intracellular core protein and its quantification.

Featured Recommendations

•Related Screening Libraries:

•Related Small Molecules:

•Related Proteins:

View All Endogenous Metabolite Isoform Specific Products:

View All Isoforms

Human Endogenous Metabolite Microbial Metabolite

View All HIV Isoform Specific Products:

View All Isoforms

HIV HIV-1 HIV-2

Biological Activity
Purity & Documentation
References
Customer Review

Description

Isochlorogenic acid A (3,5-Dicaffeoylquinic acid) is a natural phenolic acid with anti-mutagenicity, anti-HBV, anti-HIV, anti-oxidant, anti-bacterial, and anti-inflammatoryy activities^[1].

Cellular Effect

Cell Line	Type	Value	Description	References
HeLa	IC₅₀	>500 μM Compound: RLE30	Inhibition of HDAC in human HeLa cells using Boc-Lys(AC)-AMC as substrate after 24 to 48 hrs by spectrofluorometry Inhibition of HDAC in human HeLa cells using Boc-Lys(AC)-AMC as substrate after 24 to 48 hrs by spectrofluorometry	[PMID: 26996372]
HT-22	EC₅₀	>100 μM Compound: 25	Neuroprotective activity against glutamate-induced cell death in mouse HT-22 cells assessed as increase in cell viability after 24 hrs by EZ-Cytox assay Neuroprotective activity against glutamate-induced cell death in mouse HT-22 cells assessed as increase in cell viability after 24 hrs by EZ-Cytox assay	[PMID: 32991171]
MT2	ED₅₀	1 μg/mL Compound: 3,5-DCQA	Antiviral activity against HIV1 LAI in human MT2 cells assessed as virus-induced cytopathic effect after 72 hrs Antiviral activity against HIV1 LAI in human MT2 cells assessed as virus-induced cytopathic effect after 72 hrs	[PMID: 11430019]
MT2	ED₅₀	1.9 μM Compound: 3,5-dicaffeoylquinic acid	Antiviral activity against HIV1 LAI in MT2 cells assessed as inhibition of virus-induced cytopathic effect Antiviral activity against HIV1 LAI in MT2 cells assessed as inhibition of virus-induced cytopathic effect	[PMID: 18674899]
MT2	ED₅₀	2 μM Compound: 3,5-DCQA	Concentration that inhibits Human Immunodeficiency Virus Type 1 (HIV-1)-induced death of MT-2 cells Concentration that inhibits Human Immunodeficiency Virus Type 1 (HIV-1)-induced death of MT-2 cells	[PMID: 9986720]
MT2	IC₅₀	1.9 μM Compound: 8	Inhibition of HIV1 integrase activity assessed as suppression of viral replication in human MT2 cells Inhibition of HIV1 integrase activity assessed as suppression of viral replication in human MT2 cells	[PMID: 27676157]

In Vitro

Isochlorogenic acid A inhibits the aminoacylation activity of LeuRS from Giardia lamblia (GlLeuRS), with an IC₅₀ of 5.82 μg/mL^[1].
Isochlorogenic acid A (5-100 μM, 48 h) increases melanin content in B16 cells, and increases TYR proteins activity^[4].
Isochlorogenic acid A shows DPPH (IC₅₀=4.26 µg/mL), ABTS radical scavenging activity^[6].
Isochlorogenic acid A (0-250 μg/mL, 24 h) inhibits nitric oxide production in LPS-stimulated RAW 264.7 cells^[6].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Western Blot Analysis^[4]

Cell Line:	B16 cells
Concentration:	48 h
Incubation Time:	5, 50, and 100 μM
Result:	Increased tyrosinase (TYR), TRP1, TRP2, p-MITF, and total MITF protein expressions. Induced the phosphorylation of Akt at Thr308.

In Vivo

Isochlorogenic acid A (5 and 10 mg/kg, p.o., once a day for 3 weeks) ameliorates cognitive impairment induced by TMT in ICR male mice^[5].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	TMT-induced ICR male mice^[5]
Dosage:	5 and 10 mg/kg
Administration:	p.o., once a day for 3 weeks
Result:	Improved spatial memory and learning ability of mice in MWM test. Reduced the TMT-induced increased AChE activity. Reduced the MDA content compared to the TMT group.

Molecular Weight

516.45

Formula

C₂₅H₂₄O₁₂

CAS No.

2450-53-5

Appearance

Solid

Color

White to light yellow

SMILES

OC(C=C1/C=C/C(O[C@H]2[C@@H]([C@@H](C[C@](O)(C2)C(O)=O)OC(/C=C/C3=CC(O)=C(C=C3)O)=O)O)=O)=C(C=C1)O

Structure Classification

Ketones, Aldehydes, Acids

Initial Source

Endogenous metabolite

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Solvent & Solubility

In Vitro:

DMSO : ≥ 50 mg/mL (96.81 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	1.9363 mL	9.6815 mL	19.3630 mL
5 mM	0.3873 mL	1.9363 mL	3.8726 mL
10 mM	0.1936 mL	0.9681 mL	1.9363 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (4.84 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (4.84 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (4.84 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 99.53%

Select Batch:

Data Sheet (277 KB) SDS (393 KB)

COA (247 KB) HNMR (153 KB) LCMS (99 KB)

Handling Instructions (2659 KB)

References

[1]. Zhang YH, et al. 3,5-Dicaffeoylquinic acid isolated from Artemisia argyi and its ester derivatives exert anti-leucyl-tRNA synthetase of Giardia lamblia (GlLeuRS) and potential anti-giardial effects. Fitoterapia. 2012 Oct;83(7):1281-5. [Content Brief]

[2]. Malarz J, et al. Long-term cultured hairy roots of chicory-a rich source of hydroxycinnamates and 8-deoxylactucin glucoside. Appl Biochem Biotechnol. 2013 Dec;171(7):1589-601. [Content Brief]

[3]. Wan C, et al. Isolation and identification of phenolic compounds from Gynura divaricata leaves. Pharmacogn Mag. 2011 Apr;7(26):101-8. [Content Brief]

[4]. Mamat N, et al. Isochlorogenic acid A promotes melanin synthesis in B16 cell through the β-catenin signal pathway. Acta Biochim Biophys Sin (Shanghai). 2017 Sep 1;49(9):800-807. [Content Brief]

[5]. Kang JY, et al. Reversal of Trimethyltin-Induced Learning and Memory Deficits by 3,5-Dicaffeoylquinic Acid. Oxid Med Cell Longev. 2016;2016:6981595. [Content Brief]

[6]. Hong, S., et al. Antioxidant and anti-inflammatory activities of 3,5-dicaffeoylquinic acid isolated from Ligularia fischeri leaves. Food Sci Biotechnol 24, 257–263 (2015).

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	1.9363 mL	9.6815 mL	19.3630 mL	48.4074 mL
	5 mM	0.3873 mL	1.9363 mL	3.8726 mL	9.6815 mL
	10 mM	0.1936 mL	0.9681 mL	1.9363 mL	4.8407 mL
	15 mM	0.1291 mL	0.6454 mL	1.2909 mL	3.2272 mL
	20 mM	0.0968 mL	0.4841 mL	0.9681 mL	2.4204 mL
	25 mM	0.0775 mL	0.3873 mL	0.7745 mL	1.9363 mL
	30 mM	0.0645 mL	0.3227 mL	0.6454 mL	1.6136 mL
	40 mM	0.0484 mL	0.2420 mL	0.4841 mL	1.2102 mL
	50 mM	0.0387 mL	0.1936 mL	0.3873 mL	0.9681 mL
	60 mM	0.0323 mL	0.1614 mL	0.3227 mL	0.8068 mL
	80 mM	0.0242 mL	0.1210 mL	0.2420 mL	0.6051 mL

Isochlorogenic acid A Related Classifications

Neurological Disease Inflammation/Immunology

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Your Recently Viewed Products:

Product Name

Requested Quantity *

Applicant Name *

Salutation

Email Address *

Phone Number *

Department

Organization Name *

City

State

Country or Region *

Remarks

Product Name

Lot #

Applicant Name *

Email Address *

Phone Number

Department *

Organization Name *

Country or Region *

Remarks

Name
Email *

	Sorry, but the email address you supplied was invalid.

Isochlorogenic acid A (Synonyms: 3,5-Dicaffeoylquinic acid; 3,5-CQA)

Customer Review

Other Forms of Isochlorogenic acid A:

Isochlorogenic acid A Related Antibodies

13 Publications Citing Use of MCE Isochlorogenic acid A

Featured Recommendations

•Related Screening Libraries:

•Related Small Molecules:

•Related Proteins:

View All Endogenous Metabolite Isoform Specific Products:

View All HIV Isoform Specific Products:

Biological Activity

Purity & Documentation

References

Customer Review

Isochlorogenic acid A Related Antibodies

Molarity Calculator

Dilution Calculator

Complete Stock Solution Preparation Table

Isochlorogenic acid A Related Classifications

Keywords:

Your Recently Viewed Products:

Customer Review

Request for HNMR Report

SAFETY DATA SHEET (SDS)

Request for HNMR Report