Cat. No.: HY-N0056

Isochlorogenic acid A

(Synonyms: 3,5-Dicaffeoylquinic acid; 3,5-CQA)

15 Cited Publications

Customer Review

Based on 15 publication(s) in Google Scholar

Isochlorogenic acid A (3,5-Dicaffeoylquinic acid) is a natural phenolic acid with anti-mutagenicity, anti-HBV, anti-HIV, anti-oxidant, anti-bacterial, and anti-inflammatoryy activities.

For research use only. We do not sell to patients.

Purity: 99.53%
CAS No.: 2450-53-5
Formula: C₂₅H₂₄O₁₂
Molecular Weight:516.45

Storage:

Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month

Isochlorogenic acid A Chemical Structure

CAS No.: 2450-53-5

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Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- Cellular Effect
- In Vitro
- In Vivo
Chemical Information
Publications (15)
Solvent & Solubility
- In Vitro
- In Vivo
Purity & Documentation
References
Help & FAQs
Classifications
Bioz

Biological Activity

Description

Isochlorogenic acid A (3,5-Dicaffeoylquinic acid) is a natural phenolic acid with anti-mutagenicity, anti-HBV, anti-HIV, anti-oxidant, anti-bacterial, and anti-inflammatoryy activities^[1].

All Endogenous Metabolite Isoforms :

All HIV Isoforms :

Cellular Effect

Cell Line	Type	Value	Description	References
HeLa	IC₅₀	>500 μM Compound: RLE30	Inhibition of HDAC in human HeLa cells using Boc-Lys(AC)-AMC as substrate after 24 to 48 hrs by spectrofluorometry Inhibition of HDAC in human HeLa cells using Boc-Lys(AC)-AMC as substrate after 24 to 48 hrs by spectrofluorometry	[PMID: 26996372]
HT-22	EC₅₀	>100 μM Compound: 25	Neuroprotective activity against glutamate-induced cell death in mouse HT-22 cells assessed as increase in cell viability after 24 hrs by EZ-Cytox assay Neuroprotective activity against glutamate-induced cell death in mouse HT-22 cells assessed as increase in cell viability after 24 hrs by EZ-Cytox assay	[PMID: 32991171]
MT2	ED₅₀	1 μg/mL Compound: 3,5-DCQA	Antiviral activity against HIV1 LAI in human MT2 cells assessed as virus-induced cytopathic effect after 72 hrs Antiviral activity against HIV1 LAI in human MT2 cells assessed as virus-induced cytopathic effect after 72 hrs	[PMID: 11430019]
MT2	ED₅₀	1.9 μM Compound: 3,5-dicaffeoylquinic acid	Antiviral activity against HIV1 LAI in MT2 cells assessed as inhibition of virus-induced cytopathic effect Antiviral activity against HIV1 LAI in MT2 cells assessed as inhibition of virus-induced cytopathic effect	[PMID: 18674899]
MT2	ED₅₀	2 μM Compound: 3,5-DCQA	Concentration that inhibits Human Immunodeficiency Virus Type 1 (HIV-1)-induced death of MT-2 cells Concentration that inhibits Human Immunodeficiency Virus Type 1 (HIV-1)-induced death of MT-2 cells	[PMID: 9986720]
MT2	IC₅₀	1.9 μM Compound: 8	Inhibition of HIV1 integrase activity assessed as suppression of viral replication in human MT2 cells Inhibition of HIV1 integrase activity assessed as suppression of viral replication in human MT2 cells	[PMID: 27676157]

In Vitro

Isochlorogenic acid A inhibits the aminoacylation activity of LeuRS from Giardia lamblia (GlLeuRS), with an IC₅₀ of 5.82 μg/mL^[1].
Isochlorogenic acid A (5-100 μM, 48 h) increases melanin content in B16 cells, and increases TYR proteins activity^[4].
Isochlorogenic acid A shows DPPH (IC₅₀=4.26 µg/mL), ABTS radical scavenging activity^[6].
Isochlorogenic acid A (0-250 μg/mL, 24 h) inhibits nitric oxide production in LPS-stimulated RAW 264.7 cells^[6].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Western Blot Analysis^[4]

Cell Line:

B16 cells
Concentration:

48 h
Incubation Time:

5, 50, and 100 μM
Result:

Increased tyrosinase (TYR), TRP1, TRP2, p-MITF, and total MITF protein expressions.
Induced the phosphorylation of Akt at Thr308.

In Vivo

Isochlorogenic acid A (5 and 10 mg/kg, p.o., once a day for 3 weeks) ameliorates cognitive impairment induced by TMT in ICR male mice^[5].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:

TMT-induced ICR male mice^[5]
Dosage:

5 and 10 mg/kg
Administration:

p.o., once a day for 3 weeks
Result:

Improved spatial memory and learning ability of mice in MWM test.
Reduced the TMT-induced increased AChE activity.
Reduced the MDA content compared to the TMT group.

Chemical Information

CAS No. 2450-53-5
Appearance Solid
Molecular Weight 516.45
Formula C₂₅H₂₄O₁₂
Color White to light yellow
SMILES

OC(C=C1/C=C/C(O[C@H]2[C@@H]([C@@H](C[C@](O)(C2)C(O)=O)OC(/C=C/C3=CC(O)=C(C=C3)O)=O)O)=O)=C(C=C1)O
Synonyms

3,5-Dicaffeoylquinic acid; 3,5-CQA
Structure Classification
- Phenols
- Polyphenols
- Ketones, Aldehydes, Acids
Initial Source
- Endogenous metabolite
Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Publications (15)

All Cell Proliferation/Viability AssayWB

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Journal Impact Factor

Phytomedicine

A novel mechanism of Reduning injection in sepsis treatment: Targeting inflammatory kinases TBK1 and IKKβ. [Abstract]

2026 Feb:151:157776. PMID: 41512388
Cell Biosci

Coffee as a dietary strategy to prevent SARS-CoV-2 infection. [Abstract]

2023 Nov 14;13(1):210. PMID: 37964389
Food Biosci

2023 Oct, 55, 103069.
Foods

Effects of Cooking Methods on Caffeoylquinic Acids and Radical Scavenging Activity of Sweet Potato. [Abstract]

2024 Apr 3;13(7):1101. PMID: 38611405
ACS Omega

Selective Enhancement of Caffeoylquinic Acid Derivative via UV Irradiation and Validation of Analytical Method in the Aerial Aster × chusanensis Y. S. Lim. [Abstract]

2025 Sep 22;10(38):44260-44269. PMID: 41048756
Molecules

Evaluation of Environmental Factor Effects on the Polyphenol and Flavonoid Content in the Leaves of Chrysanthemum indicum L. and Its Habitat Suitability Prediction Mapping. [Abstract]

2024 Feb 20;29(5):927. PMID: 38474439
Antiviral Res

Isochlorogenic acid A impairs hepatitis B virus replication by interference with various steps of hepatitis B virus life cycle involving HO-1-mediated ROS modulation. [Abstract]

2026 Jan:245:106323. PMID: 41349371

Isochlorogenic acid A purchased from MedChemExpress. Usage Cited in: Antiviral Res. 2026 Jan:245:106323. [Abstract]

Cytotoxicity assay of Isochlorogenic acid A (ICAA) on HepAD38 and HepG2 cells. Cells were incubated with different concentrations (up to 1000 μM) of ICAA for 6 days, and the toxicity was assessed using a cell viability assay (Presto Blue).

Isochlorogenic acid A purchased from MedChemExpress. Usage Cited in: Antiviral Res. 2026 Jan:245:106323. [Abstract]

Isochlorogenic acid A (ICAA: 200 μM). Representative images from Western blots showing the intracellular core protein and its quantification.
J Funct Foods

2021, 104400.
Food Sci Biotechnol

Synergic effect in the reduction of serum uric acid level between ethanol extract of Aster glehni and vitamin B6. [Abstract]

2018 Jun 8;27(5):1439-1444. PMID: 30319854
Vet Microbiol

The Chinese medicine monomer Schisandrin C inhibits PRRSV infection by regulating the OGT-PI3K/AKT/mTOR signaling pathway. [Abstract]

2026 May:316:110992. PMID: 41865607
SSRN

2026 Apr 14.
SSRN

2026 Apr 8.
Chemistry of Plant Raw Material

2025.
Pharmacological Research-Modern Chinese Medicine

2023 Dec, 9, 100340.
SSRN

2023 May 19.

Solvent & Solubility

In Vitro:

DMSO : ≥ 50 mg/mL (96.81 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

* "≥" means soluble, but saturation unknown.

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

1.9363 mL

9.6815 mL

19.3630 mL

5 mM

0.3873 mL

1.9363 mL

3.8726 mL

10 mM

0.1936 mL

0.9681 mL

1.9363 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Concentration Concentration

Volume Volume

Molecular Weight Molecular Weight

Dilution Calculator

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

Concentration _(start) Concentration _(start)

Volume _(start) Volume _(start)

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Volume _(final) Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline

Solubility: ≥ 2.5 mg/mL (4.84 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)

Solubility: ≥ 2.5 mg/mL (4.84 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil

Solubility: ≥ 2.5 mg/mL (4.84 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

%
DMSO +

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
%
+
+
%
Tween-80 + +
%
Saline +

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Working solution concentration: 0.22 mg/mL

Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (277 KB)
SDS (394 KB)
- English - EN (394 KB)
- Français - FR (394 KB)
- Deutsch - DE (394 KB)
- Norwegian - NO (394 KB)
- Español - ES (394 KB)
- Swedish - SV (394 KB)
- Italian - IT (394 KB)
- Korean - KR (394 KB)
- Portuguese - PT (394 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Zhang YH, et al. 3,5-Dicaffeoylquinic acid isolated from Artemisia argyi and its ester derivatives exert anti-leucyl-tRNA synthetase of Giardia lamblia (GlLeuRS) and potential anti-giardial effects. Fitoterapia. 2012 Oct;83(7):1281-5. [Content Brief]

[2]. Malarz J, et al. Long-term cultured hairy roots of chicory-a rich source of hydroxycinnamates and 8-deoxylactucin glucoside. Appl Biochem Biotechnol. 2013 Dec;171(7):1589-601. [Content Brief]

[3]. Wan C, et al. Isolation and identification of phenolic compounds from Gynura divaricata leaves. Pharmacogn Mag. 2011 Apr;7(26):101-8. [Content Brief]

[4]. Mamat N, et al. Isochlorogenic acid A promotes melanin synthesis in B16 cell through the β-catenin signal pathway. Acta Biochim Biophys Sin (Shanghai). 2017 Sep 1;49(9):800-807. [Content Brief]

[5]. Kang JY, et al. Reversal of Trimethyltin-Induced Learning and Memory Deficits by 3,5-Dicaffeoylquinic Acid. Oxid Med Cell Longev. 2016;2016:6981595. [Content Brief]

[6]. Hong, S., et al. Antioxidant and anti-inflammatory activities of 3,5-dicaffeoylquinic acid isolated from Ligularia fischeri leaves. Food Sci Biotechnol 24, 257–263 (2015).

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	1.9363 mL	9.6815 mL	19.3630 mL	48.4074 mL
	5 mM	0.3873 mL	1.9363 mL	3.8726 mL	9.6815 mL
	10 mM	0.1936 mL	0.9681 mL	1.9363 mL	4.8407 mL
	15 mM	0.1291 mL	0.6454 mL	1.2909 mL	3.2272 mL
	20 mM	0.0968 mL	0.4841 mL	0.9681 mL	2.4204 mL
	25 mM	0.0775 mL	0.3873 mL	0.7745 mL	1.9363 mL
	30 mM	0.0645 mL	0.3227 mL	0.6454 mL	1.6136 mL
	40 mM	0.0484 mL	0.2420 mL	0.4841 mL	1.2102 mL
	50 mM	0.0387 mL	0.1936 mL	0.3873 mL	0.9681 mL
	60 mM	0.0323 mL	0.1614 mL	0.3227 mL	0.8068 mL
	80 mM	0.0242 mL	0.1210 mL	0.2420 mL	0.6051 mL

Keywords

Isochlorogenic acid A

15 Cited Publications

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Isochlorogenic acid A

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Publications Citing Use of MedChemExpress (MCE) Isochlorogenic acid A

All Endogenous Metabolite Isoforms

All HIV Isoforms

Biological Activity

Chemical Information

Publications (15)

Solvent & Solubility

Purity & Documentation

References

Help & FAQs

Complete Stock Solution Preparation Table

Isochlorogenic acid A Related Classifications

HY-N0056 Related Classifications