Chemical Constituents from the Aerial Parts of Elsholtzia ciliata and Their Protective Activities on Glutamate-Induced HT22 Cell Death

  • J Nat Prod. 2020 Oct 23;83(10):3149-3155. doi: 10.1021/acs.jnatprod.0c00756.
Young Hye Seo  1 ,  Tuy An Trinh  2 ,  Seung Mok Ryu  1 ,  Hyo Seon Kim  1 ,  Goya Choi  1 ,  Byeong Cheol Moon  1 ,  Sang Hee Shim  3 ,  Dae Sik Jang  4 ,  Dongho Lee  5 ,  Ki Sung Kang  2 ,  Jun Lee  1  6
Affiliations
  • 1. Herbal Medicine Resources Research Center, Korea Institute of Oriental Medicine (KIOM), Naju 58245, Republic of Korea.
  • 2. College of Korean Medicine, Gachon University, Seongnam 13120, Republic of Korea.
  • 3. College of Pharmacy, Duksung Women's University, Seoul 01369, Republic of Korea.
  • 4. Department of Life and Nanopharmaceutical Sciences, College of Pharmacy, Kyung Hee University, Seoul 02447, Republic of Korea.
  • 5. Department of Biosystems and Biotechnology, College of Life Sciences and Biotechnology, Korea University, Seoul 02841, Republic of Korea.
  • 6. University of Science & Technology (UST), Korean Convergence Medicine Major KIOM, Daejeon 34054, Republic of Korea.
PMID: 32991171 DOI: 10.1021/acs.jnatprod.0c00756
Abstract

A new phenolic glucoside, (7E,9E)-3-hydroxyavenalumic acid-3-O-[6'-O-(E)-caffeoyl]-β-d-glucopyranoside (1), and three new acetylated flavone glycosides, acacetin-7-O-[β-d-glucopyranosyl(1″″→2″)-4‴-O-acetyl-α-l-rhamnopyranosyl(1‴→6″)]-β-d-glucopyranoside (3), acacetin-7-O-[6″″-O-acetyl-β-d-glucopyranosyl(1″″→2″)-3‴-O-acetyl-α-l-rhamnopyranosyl(1‴→6″)]-β-d-glucopyranoside (5), and acacetin-7-O-[3″″,6″″-di-O-acetyl-β-d-glucopyranosyl(1″″→2″)-4‴-O-acetyl-α-l-rhamnopyranosyl(1‴→6″)]-β-d-glucopyranoside (7), as well as 34 known compounds (2, 4, 6, and 8-38) were isolated from the aerial parts of Elsholtzia ciliata. The chemical structures of the new compounds were determined by spectroscopic/spectrometric data interpretation using NMR and HRESIMS. The neuroprotective effect of the isolated compounds was evaluated by a cell viability assay on HT22 murine hippocampal neuronal cells. Among them, 23 compounds, including new substances 1 and 3, exhibited neuroprotective effects against glutamate-induced HT22 cell death. In particular, compounds 2, 16, 17, 20, 22, 28, 29, and 31 presented potent neuroprotective effects with EC50 values of 1.5-8.3 μM.

Products