1. Cell Cycle/DNA Damage
  2. Deubiquitinase
  3. 6RK73

6RK73 

Cat. No.: HY-133118
Handling Instructions

6RK73 is a covalent irreversible and specific UCHL1 inhibitor with an IC50 of 0.23 µM. 6RK73 shows almost no inhibition of UCHL3 (IC50=236 µM). 6RK73 specifically inhibit UCHL1 activity in breast cancer.

For research use only. We do not sell to patients.

6RK73 Chemical Structure

6RK73 Chemical Structure

CAS No. : 1895050-66-4

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Description

6RK73 is a covalent irreversible and specific UCHL1 inhibitor with an IC50 of 0.23 µM. 6RK73 shows almost no inhibition of UCHL3 (IC50=236 µM). 6RK73 specifically inhibit UCHL1 activity in breast cancer[1].

IC50 & Target

IC50: 0.23 µM (UCHL1), 236 µM (UCHL3)[1]

In Vitro

6RK73 (5 µM; 1-3 hours) treatment displays strong inhibition of the TGFβ-induced pSMAD2 and pSMAD3, and a decrease of TβRI and total SMAD protein levels in MDA-MB-436 cells[1].
6RK73(5 µM; 24-48 hours) results migration significantly slower than the DMSO control group in MDA-MB-436 cells[1].

Cell Viability Assay[1]

Cell Line: MDA-MB-436 cells
Concentration: 5 µM
Incubation Time: 24, 48 hours
Result: Migrated significantly slower than the DMSO control group

Western Blot Analysis[1]

Cell Line: MDA-MB-436 cells
Concentration: 5 µM
Incubation Time: 1, 2, 3 hours
Result: Displayed strong inhibition of the TGFβ-induced pSMAD2 and pSMAD3, and a decrease of TβRI and total SMAD protein levels.
In Vivo

6RK73 displays a potent inhibition of breast cancer extravasation in zebrafish[1].

Molecular Weight

307.37

Formula

C₁₃H₁₇N₅O₂S

CAS No.

1895050-66-4

SMILES

O=C([[email protected]@H]1CN(C#N)CC1)NC2=NC=C(N3CCOCC3)S2

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

References
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Keywords:

6RK73DeubiquitinaseDUBsUCHL1breastcancerInhibitorinhibitorinhibit

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6RK73
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