2. GPCR/G Protein Neuronal Signaling Membrane Transporter/Ion Channel
  3. mAChR nAChR
  4. Arecoline hydrobromide

Arecoline hydrobromide  (Synonyms: methyl 1,2,5,6-tetrahydro-1-methyl-3-pyridinecarboxylate hydrobromide)

Cat. No.: HY-B0489 Purity: 99.90%
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Arecoline hydrobromide, a naturally brain-penetrant and orally active occurring psychoactive alkaloid, is a partial agonist of nicotinic and muscarinic acetylcholine receptor. Arecoline hydrobromide exhibits stimulation, alertness, anxiolysis and anti-parasitic effects. Arecoline hydrobromide also can induce oxidative stress.

For research use only. We do not sell to patients.

CAS No. : 300-08-3

Size Price Stock Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
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10 mM * 1 mL in DMSO In-stock
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Customer Review

Based on 5 publication(s) in Google Scholar

Other Forms of Arecoline hydrobromide:

Arecoline hydrobromide Related Antibodies

Top Publications Citing Use of Products

    Arecoline hydrobromide purchased from MedChemExpress. Usage Cited in: Ecotoxicol Environ Saf. 2025 Mar 26:294:118108.  [Abstract]

    Arecoline exhibited a relatively strong binding affinity to PTGS2 in vitro, with a KD value of 10.47 μM.

    Arecoline hydrobromide purchased from MedChemExpress. Usage Cited in: Vet Microbiol. 2025 Dec 15:312:110818.  [Abstract]

    Arecoline (i.p.; single dose) stimulation led to a significant decrease in hepatic and intestinal GSH, SOD, and CAT activities and a significant increase in the MDA content, which were reversed by eFT508 (i.g.; 12 h) administration in BALB/c mice.

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    • Biological Activity

    • Purity & Documentation

    • References

    • Customer Review

    Description

    Arecoline hydrobromide, a naturally brain-penetrant and orally active occurring psychoactive alkaloid, is a partial agonist of nicotinic and muscarinic acetylcholine receptor. Arecoline hydrobromide exhibits stimulation, alertness, anxiolysis and anti-parasitic effects. Arecoline hydrobromide also can induce oxidative stress[1][2][3][4][5].

    IC50 & Target

    nAChR/mAChR[3]
    In Vitro

    Arecoline hydrobromide induces the generation of reactive oxygen species and cell cycle arrest at the G1/G0 phase in HaCaT cells without affecting the expression of p21/Cip1. Arecoline hydrobromide-induced epithelial cell death at higher concentrations is caused by oxidative trauma without eliciting apoptosis. Arecoline hydrobromide upregulates the expression of the following stress-responsive genes: hemeoxygenase-1; ferritin light chain; glucose-6-phosphate dehydrogenase; glutamatecysteine ligase catalytic subunit; and glutathione reductase[1].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Arecoline hydrobromide Related Antibodies
    In Vivo

    Arecoline (0.2% in water; p.o., 2 weeks) hydrobromide alleviates the anxiety and depression in CFA (HY-153808)-induced mice[5].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
    Molecular Weight

    236.11

    Formula

    C8H14BrNO2
    CAS No.
    Appearance

    Solid

    Color

    White to light yellow

    SMILES

    O=C(C1=CCCN(C)C1)OC.Br
    Structure Classification
    Initial Source
    Shipping

    Room temperature in continental US; may vary elsewhere.
    Storage

    4°C, sealed storage, away from moisture

    *In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

    Solvent & Solubility
    In Vitro: 

    H2O : 50 mg/mL (211.77 mM; Need ultrasonic)

    DMSO : ≥ 50 mg/mL (211.77 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

    *"≥" means soluble, but saturation unknown.

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 4.2353 mL 21.1766 mL 42.3531 mL
    5 mM 0.8471 mL 4.2353 mL 8.4706 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    * Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

    • Molarity Calculator

    • Dilution Calculator

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    Concentration (start)

    C1

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    Volume (start)

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    In Vivo:

    Select the appropriate dissolution method based on your experimental animal and administration route.

    For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
    To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

      Solubility: ≥ 2.08 mg/mL (8.81 mM); Clear solution

      This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

      Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
    • Protocol 2

      Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

      Solubility: ≥ 2.08 mg/mL (8.81 mM); Clear solution

      This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

      Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.

    For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  PBS

      Solubility: 100 mg/mL (423.53 mM); Clear solution; Need ultrasonic

    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

    Dosage

    mg/kg

    Animal weight
    (per animal)

    g

    Dosing volume
    (per animal)

    μL

    Number of animals

    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Calculation results:
    Working solution concentration: mg/mL
    This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
    The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
    Purity & Documentation

    Purity: 99.95%

    SDS (396 KB)

    COA (243 KB) HNMR (226 KB) LCMS (104 KB)

    Handling Instructions (2659 KB)
    References

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    H2O / DMSO 1 mM 4.2353 mL 21.1766 mL 42.3531 mL 105.8829 mL
    5 mM 0.8471 mL 4.2353 mL 8.4706 mL 21.1766 mL
    10 mM 0.4235 mL 2.1177 mL 4.2353 mL 10.5883 mL
    15 mM 0.2824 mL 1.4118 mL 2.8235 mL 7.0589 mL
    20 mM 0.2118 mL 1.0588 mL 2.1177 mL 5.2941 mL
    25 mM 0.1694 mL 0.8471 mL 1.6941 mL 4.2353 mL
    30 mM 0.1412 mL 0.7059 mL 1.4118 mL 3.5294 mL
    40 mM 0.1059 mL 0.5294 mL 1.0588 mL 2.6471 mL
    50 mM 0.0847 mL 0.4235 mL 0.8471 mL 2.1177 mL
    60 mM 0.0706 mL 0.3529 mL 0.7059 mL 1.7647 mL
    80 mM 0.0529 mL 0.2647 mL 0.5294 mL 1.3235 mL
    100 mM 0.0424 mL 0.2118 mL 0.4235 mL 1.0588 mL

    * Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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    Arecoline hydrobromide Related Classifications

    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    Arecoline300-08-3methyl 1,2,5,6-tetrahydro-1-methyl-3-pyridinecarboxylatemAChRnAChRMuscarinic acetylcholine receptorNicotinic acetylcholine receptorsalkaloidnicotinicmuscarinicacetylcholinestimulationalertnessanxiolysisparasiticoxidativestressInhibitorinhibitorinhibit

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