Catalpol (Synonyms: Catalpinoside)

Name: Catalpol
Brand: MedChemExpress
SKU: HY-N0820
Rating: 5.0 (13 reviews)

Cat. No.: HY-N0820 Purity: 99.86%: Data Sheet
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Catalpol (Catalpinoside), an iridoid glycoside found in Rehmannia glutinosa. Catalpol has neuroprotective, hypoglycemic, anti-inflammatory, anti-cancer, anti-spasmodic, anti-oxidant effects and anti-HBV effects.

For research use only. We do not sell to patients.

CAS No. : 2415-24-9

Size	Stock	Quantity

Free Sample (0.1 - 0.2 mg)	Apply Now
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	In-stock	Estimated Time of Arrival: December 31
Solid
25 mg	In-stock	Estimated Time of Arrival: December 31
50 mg	In-stock	Estimated Time of Arrival: December 31
100 mg	In-stock	Estimated Time of Arrival: December 31
200 mg	Get quote
500 mg	Get quote

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Customer Review

Based on 13 publication(s) in Google Scholar

Other Forms of Catalpol:

Catalpol (Standard) Get quote

13 Publications Citing Use of MCE Catalpol

Bio/Physico-chemical Assay

: Catalpol purchased from MedChemExpress. Usage Cited in: Bioeng Transl Med. 2024 Dec 29;10(3):e10746.; Organoids (generation of cerebral organoids; 22 D; 10 uM). Immunofluorescence staining for TBR1 (red), SOX2 (green), and DAPI (blue) in day 30 control and Catalpol-treated organoids.

: Catalpol purchased from MedChemExpress. Usage Cited in: Sci Rep. 2024 Nov 16;14(1):28327. [Abstract]; Catalpol (HUVECs; 1, 10, 100 µM; for 24 h). Western blots were conducted to detect the Bax, Bcl2, Cyto C, C-casp3 expression level. β-Actin was used as the endogenous control.

: Catalpol purchased from MedChemExpress. Usage Cited in: J Ethnopharmacol. 2024 May 6:331:118272. [Abstract]; Bar charts showing the length of the regenerating fin of zebrafish exposure to different concentrations of Catalpol (2 days) at 2 dpa, n = 8~20. The results indicated that 0.5 mg/L was the optimal concentration for Catalpol to exert its pro-regenerative effect.

: Catalpol purchased from MedChemExpress. Usage Cited in: Acta Pharmacol Sin. 2022 Jul;43(7):1670-1685. [Abstract]; The numbers of vessels and neurons in the cortex near the infarcted area were up-regulated in Catalpol (Rat; i.v, 2.5, 5.0, 10.0 mg/kg/d; for 14 d) groups.

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Biological Activity
Purity & Documentation
References
Customer Review

Description

Cellular Effect

Cell Line	Type	Value	Description	References
A2780	GI₅₀	> 100 μM Compound: 1, catalpol	Antiproliferative activity against human A2780 cells after 48 hrs by SRB assay Antiproliferative activity against human A2780 cells after 48 hrs by SRB assay	[PMID: 17178223]
A2780	GI₅₀	> 100 μM Compound: 2	Antiproliferative activity against human A2780 cells after 48 hrs by sulforhodamine B assay Antiproliferative activity against human A2780 cells after 48 hrs by sulforhodamine B assay	[PMID: 20231098]
A2780	IC₅₀	> 10 μM Compound: 10	Cytotoxicity against human A2780 cells by MTT assay Cytotoxicity against human A2780 cells by MTT assay	[PMID: 26859776]
A549	IC₅₀	> 10 μM Compound: 10	Cytotoxicity against human A549 cells by MTT assay Cytotoxicity against human A549 cells by MTT assay	[PMID: 26859776]
BGC-823	IC₅₀	> 10 μM Compound: 10	Cytotoxicity against human BGC823 cells by MTT assay Cytotoxicity against human BGC823 cells by MTT assay	[PMID: 26859776]
BV-2	IC₅₀	7.3 μM Compound: 10	Inhibition of LPS-induced NO production in mouse BV2 cells assessed as reduction in nitrite level pretreated for 24 hrs before LPS treatment for additional 24 hrs by Griess reaction method Inhibition of LPS-induced NO production in mouse BV2 cells assessed as reduction in nitrite level pretreated for 24 hrs before LPS treatment for additional 24 hrs by Griess reaction method	[PMID: 26859776]
Bel-7402	IC₅₀	> 10 μM Compound: 10	Cytotoxicity against human Bel7402 cells by MTT assay Cytotoxicity against human Bel7402 cells by MTT assay	[PMID: 26859776]
HBL-100	GI₅₀	> 100 μM Compound: 2	Antiproliferative activity against human HBL100 cells after 48 hrs by sulforhodamine B assay Antiproliferative activity against human HBL100 cells after 48 hrs by sulforhodamine B assay	[PMID: 20231098]
HT-29	IC₅₀	> 10 μM Compound: 10	Cytotoxicity against human HT-29 cells by MTT assay Cytotoxicity against human HT-29 cells by MTT assay	[PMID: 26859776]
HeLa	GI₅₀	> 100 μM Compound: 2	Antiproliferative activity against human HeLa cells after 48 hrs by sulforhodamine B assay Antiproliferative activity against human HeLa cells after 48 hrs by sulforhodamine B assay	[PMID: 20231098]
SW1573	GI₅₀	> 100 μM Compound: 1, catalpol	Antiproliferative activity against human SW1573 cells after 48 hrs by SRB assay Antiproliferative activity against human SW1573 cells after 48 hrs by SRB assay	[PMID: 17178223]
SW1573	GI₅₀	> 100 μM Compound: 2	Antiproliferative activity against human SW1573 cells after 48 hrs by sulforhodamine B assay Antiproliferative activity against human SW1573 cells after 48 hrs by sulforhodamine B assay	[PMID: 20231098]
T47D	GI₅₀	> 100 μM Compound: 1, catalpol	Antiproliferative activity against human T47D cells after 48 hrs by SRB assay Antiproliferative activity against human T47D cells after 48 hrs by SRB assay	[PMID: 17178223]
T47D	GI₅₀	> 100 μM Compound: 2	Antiproliferative activity against human T47D cells after 48 hrs by sulforhodamine B assay Antiproliferative activity against human T47D cells after 48 hrs by sulforhodamine B assay	[PMID: 20231098]
WiDr	GI₅₀	> 100 μM Compound: 1, catalpol	Antiproliferative activity against human WiDr cells after 48 hrs by SRB assay Antiproliferative activity against human WiDr cells after 48 hrs by SRB assay	[PMID: 17178223]
WiDr	GI₅₀	> 100 μM Compound: 2	Antiproliferative activity against human WiDr cells after 48 hrs by sulforhodamine B assay Antiproliferative activity against human WiDr cells after 48 hrs by sulforhodamine B assay	[PMID: 20231098]

In Vitro

Catalpol (0.1 μg/mL; for 3 days) can induce neuronal differentiation in PC12 cells through activation of the intracellular signal transduction pathway, and promote neurite length^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

Catalpol (25-100 mg/kg; intraperitoneal injection; once) treatment clearly reduces blood urea nitrogen, serum creatinine levels and the expression of KIM-1 in renal ischemia/reperfusion-injury (IRI) mice. Catalpol markedly reduces the expression of PI3K, Akt and eNOS levels, and suppresses the TNF-α, IL-1β, IL-6 and IL-10 activities^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	C57BL/6 mice treated with renal ischemia/reperfusion surgery^[1]
Dosage:	25 mg/kg, 50 mg/kg and 100 mg/kg
Administration:	Intraperitoneal injection; once
Result:	Clearly reduced blood urea nitrogen, serum creatinine levels and the expression of KIM-1 in renal IRI mice.

Molecular Weight

362.33

Formula

C₁₅H₂₂O₁₀

CAS No.

2415-24-9

Appearance

Solid

Color

White to off-white

SMILES

OC[C@@]12[C@]([C@@H]3O[C@]([C@@H]([C@@H](O)[C@@H]4O)O)([H])O[C@@H]4CO)([H])[C@](C=CO3)([H])[C@H](O)[C@@H]1O2

Structure Classification

Initial Source

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (275.99 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H₂O : 7.14 mg/mL (19.71 mM; ultrasonic and warming and heat to 60°C)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.7599 mL	13.7996 mL	27.5992 mL
5 mM	0.5520 mL	2.7599 mL	5.5198 mL
10 mM	0.2760 mL	1.3800 mL	2.7599 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.08 mg/mL (5.74 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.08 mg/mL (5.74 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.08 mg/mL (5.74 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 99.86%

Select Batch:

Data Sheet (281 KB) SDS (393 KB)

COA (257 KB) HNMR (269 KB) RP-HPLC (207 KB) MS (69 KB)

Handling Instructions (2659 KB)

References

[1]. Jili Zhu, et al. Catalpol protects mice against renal ischemia/reperfusion injury via suppressing PI3K/Akt-eNOS signaling and inflammation. Int J Clin Exp Med. 2015 Feb 15;8(2):2038-44. [Content Brief]

[2]. M Yamazaki, et al. Neuritogenic effect of natural iridoid compounds on PC12h cells and its possible relation to signaling protein kinases. Biol Pharm Bull. 1996 Jun;19(6):791-5. [Content Brief]

[3]. R Mehrotra, et al. In vitro studies on the effect of certain natural products against hepatitis B virus. Indian J Med Res. 1990 Apr;92:133-8. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

H₂O / DMSO	1 mM	2.7599 mL	13.7996 mL	27.5992 mL	68.9979 mL
	5 mM	0.5520 mL	2.7599 mL	5.5198 mL	13.7996 mL
	10 mM	0.2760 mL	1.3800 mL	2.7599 mL	6.8998 mL
	15 mM	0.1840 mL	0.9200 mL	1.8399 mL	4.5999 mL
DMSO	20 mM	0.1380 mL	0.6900 mL	1.3800 mL	3.4499 mL
	25 mM	0.1104 mL	0.5520 mL	1.1040 mL	2.7599 mL
	30 mM	0.0920 mL	0.4600 mL	0.9200 mL	2.2999 mL
	40 mM	0.0690 mL	0.3450 mL	0.6900 mL	1.7249 mL
	50 mM	0.0552 mL	0.2760 mL	0.5520 mL	1.3800 mL
	60 mM	0.0460 mL	0.2300 mL	0.4600 mL	1.1500 mL
	80 mM	0.0345 mL	0.1725 mL	0.3450 mL	0.8625 mL
	100 mM	0.0276 mL	0.1380 mL	0.2760 mL	0.6900 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Catalpol Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Catalpol2415-24-9CatalpinosideHBVHepatitis B virusanti-cancerneuroprotectiveanti-inflammatorydiuretichypoglycemichepatitisvirusiridoidglycosideInhibitorinhibitorinhibit

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Catalpol (Synonyms: Catalpinoside)

Customer Review

Other Forms of Catalpol:

Catalpol Related Antibodies

13 Publications Citing Use of MCE Catalpol

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•Related Screening Libraries:

•Related Small Molecules:

•Related Proteins:

Biological Activity

Purity & Documentation

References

Customer Review

Catalpol Related Antibodies

Molarity Calculator

Dilution Calculator

Complete Stock Solution Preparation Table

Catalpol Related Classifications

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