Cefamandole sodium
Based on 1 Customer Validation
Cefamandole sodium (Cephamandole sodium) is a β-lactam antibiotic with broad-spectrum antibacterial activity. Cefamandole sodium resists hydrolysis by β-lactamase of some Gram-negative bacteria. Cefamandole sodium binds with high affinity to major penicillin-binding proteins (PBP) and with low affinity to PBP 2a, induces the production of low-affinity PBP associated with Methicillin (HY-121544) resistance, and blocks peptidoglycan biosynthesis through the SecA subunit of PBP. Cefamandole sodium can be used in research related to bacterial infections.
For research use only. We do not sell to patients.
- Purity : 95.0%
- CAS No.: 30034-03-8
- Formula: C18H17N6NaO5S2
- Molecular Weight:484.48
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Storage:
-20°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Protocols
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
Description
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β-lactam |
PBP |
In Vitro
Cefamandole (0.12-64 μg/mL; 18 h) sodium inhibits 90%-100% of Methicillin (HY-121544)-sensitive Staphylococcus aureus, group A and group B streptococci, Streptococcus pneumoniae, Neisseria gonorrhoeae, Haemophilus influenzae, and Salmonella typhi[3].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:Methicillin-susceptible S. aureus, group A and B streptococci, S. pneumoniae, N. gonorrhoeae, H. influenzae, and S. typhosa
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Concentration:Cefamandole:
0.12, 0.25, 0.5, 1, 2, 4, 8, 16, 32, 16 μg/mL -
Incubation Time:18 h
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Result:Inhibited 90-100% of methicillin-susceptible S. aureus, group A and B streptococci, S. pneumoniae, N. gonorrhoeae, H. influenzae, and S. typhosa at concentrations of 2 μg/mL or less, with variable activity against gram-negative rods including 70% of E. coli inhibited at 4 μg/mL and 80% of B. fragilis subsp. fragilis inhibited at 32 μg/mL.
In Vivo
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:New Zealand White (2 kg)[6]
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Dosage:100 mg/kg
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Administration:i.v.; every 4 h; six doses total (first dose 30 min before inoculation)
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Result:Resulted in infection in 9/29 (31%) rabbits infected with strain 220, 10/16 (63%) rabbits infected with strain 313, and 0/11 (0%) rabbits infected with strain W-05386.
In early-infection groups, rabbits had 70 CFU (n = 4) on the aortic valve for strain 313 and 54 CFU (n = 4) for strain W-05386 at 1.5 h after inoculation.
Chemical Information
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CAS No. 30034-03-8
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Appearance Solid
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Molecular Weight 484.48
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Formula C18H17N6NaO5S2
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Color White to off-white
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SMILES
O=C(C(N12)=C(CSC3=NN=NN3C)CS[C@]2([H])[C@H](NC([C@H](O)C4=CC=CC=C4)=O)C1=O)O[Na]
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Synonyms
Cephamandole sodium
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
-20°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
Solvent & Solubility
In Vitro:
H2O : 250 mg/mL (516.02 mM; Need ultrasonic)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
In Vivo:
For the following dissolution methods, please prepare the working solution directly:
It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: PBS
Solubility: 100 mg/mL (206.41 mM); Clear solution; Need ultrasonic
Purity & Documentation
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Data Sheet (291 KB)
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SDS (558 KB)
- English - EN (558 KB)
- Français - FR (558 KB)
- Deutsch - DE (558 KB)
- Norwegian - NO (558 KB)
- Español - ES (558 KB)
- Swedish - SV (558 KB)
- Italian - IT (558 KB)
- Korean - KR (558 KB)
- Portuguese - PT (558 KB)
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Handling Instructions (2659 KB)
References
[1]. Neu HC, et al. Cefamandole, a cephalosporin antibiotic with an unusually wide spectrum of activity. Antimicrob Agents Chemother. 1974;6(2):177-182. [Content Brief]
[2]. Eykyn S, et al. Antibacterial activity of cefamandole, a new cephalosporin antibiotic, compared with that of cephaloridine, cephalothin, and cephalexin. Antimicrob Agents Chemother. 1973;3(6):657-661. [Content Brief]
[3]. Griffith RS, et al. Cefamandole: in vitro and clinical pharmacokinetics. Antimicrob Agents Chemother. 1976;10(5):814-823. [Content Brief]
[4]. Lee FH, et al. Comparative tissue distribution of ceforanide, cefazolin, and cefamandole in rats. Antimicrob Agents Chemother. 1981;19(4):625-627. [Content Brief]
[5]. Yeh PH, et al. Determination of unbound cefamandole in rat blood by microdialysis and microbore liquid chromatography. Biomed Chromatogr. 2001;15(1):14-17. [Content Brief]
[9]. Pothineni VR, et al. In vitro and in vivo evaluation of cephalosporins for the treatment of Lyme disease. Drug Des Devel Ther. 2018 Sep 11;12:2915-2921. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
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| H2O | 1 mM | 2.0641 mL | 10.3203 mL | 20.6407 mL | 51.6017 mL |
| 5 mM | 0.4128 mL | 2.0641 mL | 4.1281 mL | 10.3203 mL | |
| 10 mM | 0.2064 mL | 1.0320 mL | 2.0641 mL | 5.1602 mL | |
| 15 mM | 0.1376 mL | 0.6880 mL | 1.3760 mL | 3.4401 mL | |
| 20 mM | 0.1032 mL | 0.5160 mL | 1.0320 mL | 2.5801 mL | |
| 25 mM | 0.0826 mL | 0.4128 mL | 0.8256 mL | 2.0641 mL | |
| 30 mM | 0.0688 mL | 0.3440 mL | 0.6880 mL | 1.7201 mL | |
| 40 mM | 0.0516 mL | 0.2580 mL | 0.5160 mL | 1.2900 mL | |
| 50 mM | 0.0413 mL | 0.2064 mL | 0.4128 mL | 1.0320 mL | |
| 60 mM | 0.0344 mL | 0.1720 mL | 0.3440 mL | 0.8600 mL | |
| 80 mM | 0.0258 mL | 0.1290 mL | 0.2580 mL | 0.6450 mL | |
| 100 mM | 0.0206 mL | 0.1032 mL | 0.2064 mL | 0.5160 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Cefamandole sodium Related Classifications
HY-B1128A Related Classifications
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Keywords
- Cefamandole
- 30034-03-8
- Cephamandole
- Antibiotic
- Bacterial
- Penicillin-binding protein (PBP)
- Beta-lactamase
- Lyme disease
- antibacterial activity
- bacterial infection
- beta-lactam antibiotic
- endocarditis
- gram-negative bacteria
- gram-positive bacteria
- methicillin resistance
- penicillin-binding proteins
- peptidoglycan biosynthesis
- Inhibitor
- inhibitor
- inhibit