Cefamandole (Synonyms: Cephamandole)

Cat. No.: HY-B1128: Data Sheet Handling Instructions
FAQs
Technical Support

Cefamandole (Cephamandole) is a semi-synthetic second-generation cephalosporin antibiotic with broad-spectrum antimicrobial activity. Cefamandole is resistant to hydrolysis by β-lactamases produced by some Gram-negative bacteria. Cefamandole kills Gram-positive cocci and various Gram-negative bacilli mainly by inhibiting cell wall synthesis, but it is inactive against Pseudomonas, Proteus vulgaris and Providencia stuartii, and its efficacy is affected by inoculum size. The plasma elimination half-life of Cefamandole in rats is only 0.4 h, it is mainly excreted in urine in biologically active form, and it hardly penetrates the non-inflamed blood-brain barrier. Cefamandole is widely used in studies related to bacterial infections.

At equivalent molar concentrations, both the salt and free forms of a compound exhibit comparable biological activity. Nevertheless, the salt form (Cefamandole sodium) usually boasts enhanced water solubility and stability.

For research use only. We do not sell to patients.

Cefamandole Chemical Structure

CAS No. : 34444-01-4

Size		Stock
MOQ		Minimum order quantity
50 mg		Get quote
100 mg		Get quote
250 mg		Get quote
Other size		Get quote

* Please select Quantity before adding items.

This product is a controlled substance and not for sale in your territory.

Other In-stock Forms of Cefamandole:

Cefamandole sodium

Other Forms of Cefamandole:

Featured Recommendations

•Related Small Molecules:

•Related Proteins:

View All Antibiotic Isoform Specific Products:

View All Isoforms

Aminoglycoside Glycopeptide Lipopeptide Macrolide Oxazolidinone Quinolone Tetracycline β-lactam

Biological Activity
Purity & Documentation
References
Customer Review

Description

IC₅₀ & Target

β-lactam

Cellular Effect

Cell Line	Type	Value	Description	References
S2	IC₅₀	1.57 mM Compound: Cefamandole	TP_TRANSPORTER: inhibition of PGF2alpha in OAT2-S2 cells TP_TRANSPORTER: inhibition of PGF2alpha in OAT2-S2 cells	[PMID: 12650826]
S2	IC₅₀	3.18 mM Compound: Cefamandole	TP_TRANSPORTER: inhibition of PGF2alpha in OAT2-S2 cells TP_TRANSPORTER: inhibition of PGF2alpha in OAT2-S2 cells	[PMID: 12650826]
S2	IC₅₀	450 μM Compound: Cefamandole	TP_TRANSPORTER: inhibition of PAH uptake in OAT1-expressing S2 cells TP_TRANSPORTER: inhibition of PAH uptake in OAT1-expressing S2 cells	[PMID: 12005172]
S2	IC₅₀	90 μM Compound: Cefamandole	TP_TRANSPORTER: inhibition of Estrone sulfate uptake in OAT3-expressing S2 cells TP_TRANSPORTER: inhibition of Estrone sulfate uptake in OAT3-expressing S2 cells	[PMID: 12005172]

In Vitro

Cefamandole (30 μg/mL; 0, 1, 3, 6, 12 h) is hydrolyzed by beta-lactamases from Enterobacter cloacae, Serratia marcescens, and Proteus morganii, allowing bacterial growth to resume after hydrolysis is complete, while Pseudomonas aeruginosa grows despite cefamandole presence prior to hydrolysis^[1].
Cefamandole (sodium) (0.12-64 μg/mL; 18 h) inhibits 90-100% of methicillin-susceptible S. aureus, group A and B streptococci, S. pneumoniae, N. gonorrhoeae, H. influenzae, and S. typhosa^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay^[3]

Cell Line:	Methicillin-susceptible S. aureus, group A and B streptococci, S. pneumoniae, N. gonorrhoeae, H. influenzae, and S. typhosa
Concentration:	0.12, 0.25, 0.5, 1, 2, 4, 8, 16, 32, 16 μg/mL
Incubation Time:	18 h
Result:	Inhibited 90-100% of methicillin-susceptible S. aureus, group A and B streptococci, S. pneumoniae, N. gonorrhoeae, H. influenzae, and S. typhosa at concentrations of 2 μg/mL or less, with variable activity against gram-negative rods including 70% of E. coli inhibited at 4 μg/mL and 80% of B. fragilis subsp. fragilis inhibited at 32 μg/mL.

In Vivo

Following subcutaneous administration of a single dose of cefamandole sodium (100 mg/kg) to male Long-Evans rats, a peak plasma concentration of 49 μg/mL is reached at 0.5 h, with a plasma half-life of 0.4 h^[4].
Cefamandole (50 mg/kg; intravenous administration; single dose) exhibits free drug pharmacokinetic characteristics consistent with a two-compartment model in male Sprague-Dawley rats, with an elimination half-life of 21.6 min^[5].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	Sprague-Dawley (male, adult, 280-350 g, specific pathogen-free)^[5]
Dosage:	50 mg/kg
Administration:	i.v.; single dose
Result:	Achieved an in vivo microdialysis recovery of 55.44% (n=6) at an infusion concentration of 1 μg/mL. Fitted a two-compartment pharmacokinetic model with parameters: A = 148.4 ± 34.3 μg/mL, B = 16.9 ± 4.2 μg/mL, α = 0.11 ± 0.01 1/min, β = 0.033 ± 0.003 1/min, distribution half-life (t₁/₂,α) = 6.4 ± 0.5 min, elimination half-life (t₁/₂,β) = 21.6 ± 1.6 min, AUC = 1799.4 ± 254.7 μg min/mL, volume of distribution (Vd) = 372.2 ± 87.4 mL, clearance (Cl) = 30.5 ± 5.0 mL/min/kg, mean residence time (MRT) = 15.4 ± 1.1 min (n=5).

Molecular Weight

462.50

Formula

C₁₈H₁₈N₆O₅S₂

CAS No.

34444-01-4

SMILES

O=C(C(N12)=C(CSC3=NN=NN3C)CS[C@]2([H])[C@H](NC([C@H](O)C4=CC=CC=C4)=O)C1=O)O

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

Purity & Documentation

Handling Instructions (2659 KB)

References

[1]. Neu HC, et al. Cefamandole, a cephalosporin antibiotic with an unusually wide spectrum of activity. Antimicrob Agents Chemother. 1974;6(2):177-182. [Content Brief]

[2]. Eykyn S, et al. Antibacterial activity of cefamandole, a new cephalosporin antibiotic, compared with that of cephaloridine, cephalothin, and cephalexin. Antimicrob Agents Chemother. 1973;3(6):657-661. [Content Brief]

[3]. Griffith RS, et al. Cefamandole: in vitro and clinical pharmacokinetics. Antimicrob Agents Chemother. 1976;10(5):814-823. [Content Brief]

[4]. Lee FH, et al. Comparative tissue distribution of ceforanide, cefazolin, and cefamandole in rats. Antimicrob Agents Chemother. 1981;19(4):625-627. [Content Brief]

[5]. Yeh PH, et al. Determination of unbound cefamandole in rat blood by microdialysis and microbore liquid chromatography. Biomed Chromatogr. 2001;15(1):14-17. [Content Brief]

Cefamandole Related Classifications

Infection

Molarity Calculator
Dilution Calculator

The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass		Concentration		Volume		Molecular Weight *
	=		×		×

The dilution calculator equation

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)	×	Volume _(start)	=	Concentration _(final)	×	Volume _(final)
	×		=		×
C1		V1		C2		V2

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Cefamandole34444-01-4CephamandoleAntibioticBacterialgram-negative beta-lactamasesStreptococcus pyogenesgram-negative bacilliProteus vulgarisProvidencia stuartiiStreptococcus viridansgram-negative cocciDiplococcus pneumoniaePseudomonas speciesgram-positive cocciInhibitorinhibitorinhibit

Your Recently Viewed Products:

Product Name

Requested Quantity *

Applicant Name *

Salutation

Email Address *

Phone Number *

Department

Organization Name *

City

State

Country or Region *

Remarks

Product Name

Lot #

Applicant Name *

Email Address *

Phone Number

Department *