Dipyridamole

Name: Dipyridamole
Brand: MedChemExpress
SKU: HY-B0312
Rating: 5.0 (11 reviews)

Cat. No.: HY-B0312 Purity: 99.93%: Data Sheet
COA

SDS
Handling Instructions
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Technical Support

Dipyridamole is an orally active phosphodiesterase (PDE) inhibitor. Dipyridamole also is an antiplatelet agent used in secondary prophylaxis against stroke. Dipyridamole can induce cancer cell-specific apoptosis.

For research use only. We do not sell to patients.

CAS No. : 58-32-2

Size	Stock	Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	In-stock	Estimated Time of Arrival: December 31
Solid
100 mg	In-stock	Estimated Time of Arrival: December 31
500 mg	In-stock	Estimated Time of Arrival: December 31
1 g	Get quote
5 g	Get quote

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Based on 11 publication(s) in Google Scholar

Other Forms of Dipyridamole:

11 Publications Citing Use of MCE Dipyridamole

Flow Cytometry

In Vivo Efficacy Study

Histological Imaging/Staining

Cell Proliferation/Viability Assay

: Dipyridamole purchased from MedChemExpress. Usage Cited in: Cell Mol Gastroenterol Hepatol. 2025 May 21:101539. [Abstract]; Representative flow cytometry plots and quantification of the percentage of BNDs and CNBs in naïve B cells treated with Dipyridamole (1 mM; 3 d) or vehicle, in the presence and absence of inosine (250 mM). Dipyridamole nearly reversed the effects of inosine, including the increased frequency of BNDs.

: Dipyridamole purchased from MedChemExpress. Usage Cited in: Cell Mol Gastroenterol Hepatol. 2025 May 21:101539. [Abstract]; Mice were injected with ConA (10 mg/kg), inosine (300 mg/kg), or a combination of both, with or without Dipyridamole (1 mg/kg; i.p.; 30 minutes before inosine administration). Representative images of H&E-stained liver sections were obtained. Dipyridamole reversed the substantial reduction in the area of hepatic necrosis in inosine-treated mice.

: Dipyridamole purchased from MedChemExpress. Usage Cited in: Invest Ophthalmol Vis Sci. 2025 Dec 1;66(15):16. [Abstract]; The representative fundus examination images and hematoxylin and eosin-stained images on day 14 after immunization in EAU and preventative Dipyridamole (DIP) (300 mg/kg/day; p.o.; 6 d) groups, with the black arrows indicating inflammatory exudation and linear lesions and the white arrows highlighting retinal folding. Preventative Dipyridamole (DIP) significantly attenuated chorioretinal lesions, infiltrations, and vasculitis.

: Dipyridamole purchased from MedChemExpress. Usage Cited in: Invest Ophthalmol Vis Sci. 2025 Dec 1;66(15):16. [Abstract]; The flow cytometry dot plots and column charts showing the percentage of CD4+ cells, CD4+IFN-γ+ and CD4+IL-17A+ cells in the live cells in retinal tissues from the EAU and preventative Dipyridamole (DIP) (300 mg/kg/day; p.o.; 6 d) groups.

: Dipyridamole purchased from MedChemExpress. Usage Cited in: Invest Ophthalmol Vis Sci. 2025 Dec 1;66(15):16. [Abstract]; Histograms of the Carboxy fluorescein succinimidyl ester fluorescence intensity and scatter plot showed the proliferation of T cells cultured in different Dipyridamole (DIP) (3-4 μM; 72 h) concentrations. Dipyridamole (DIP) dosedependently suppressed CD4+ T-cell proliferation.

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PDE1 PDE2 PDE3 PDE4 PDE5 PDE6 PDE7 PDE9 PDE10 PDE11 PDE12 PDE8 Autotaxin

Biological Activity
Purity & Documentation
References
Customer Review

Description

IC₅₀ & Target

PDE

Cellular Effect

Cell Line	Type	Value	Description	References
COR-L23	IC₅₀	15 nM Compound: 3, DP	Inhibition of ENT-mediated [3H]thymidine incorporation in human COR-L23 cells by liquid scintillation counting Inhibition of ENT-mediated [3H]thymidine incorporation in human COR-L23 cells by liquid scintillation counting	[PMID: 21366300]
HEK293	IC₅₀	2 μM Compound: Dipyridamole	TP_TRANSPORTER: inhibition of 9-(2-phosphonomethoxyethyl)adenine(PMEA) efflux (PMEA: 1 uM) in MRP4-expressing HEK293 cells TP_TRANSPORTER: inhibition of 9-(2-phosphonomethoxyethyl)adenine(PMEA) efflux (PMEA: 1 uM) in MRP4-expressing HEK293 cells	[PMID: 12695538]
HEK293	IC₅₀	30 μM Compound: Dipyridamole	TP_TRANSPORTER: inhibition of 9-(2-phosphonomethoxyethyl)adenine(PMEA) efflux (PMEA: 1 uM) in MRP5-expressing HEK293 cells TP_TRANSPORTER: inhibition of 9-(2-phosphonomethoxyethyl)adenine(PMEA) efflux (PMEA: 1 uM) in MRP5-expressing HEK293 cells	[PMID: 12695538]
K562	IC₅₀	144.8 nM Compound: Dipyridamole	Inhibition of ENT1 in human K562 cells by flow cytometric assay Inhibition of ENT1 in human K562 cells by flow cytometric assay	[PMID: 17636949]
L1210	IC₅₀	0.34 μM Compound: DP	Inhibition of [3H]thymidine uptake in L1210 cells Inhibition of [3H]thymidine uptake in L1210 cells	[PMID: 15369395]
NIH-3T3-G185	IC₅₀	22.7 μM Compound: Dipyridamole	TP_TRANSPORTER: inhibition of Daunorubicin efflux in NIH-3T3-G185 cells TP_TRANSPORTER: inhibition of Daunorubicin efflux in NIH-3T3-G185 cells	[PMID: 11716514]
NIH-3T3-G185	IC₅₀	23.7 μM Compound: Dipyridamole	TP_TRANSPORTER: inhibition of LDS-751 efflux in NIH-3T3-G185 cells TP_TRANSPORTER: inhibition of LDS-751 efflux in NIH-3T3-G185 cells	[PMID: 11716514]
NIH-3T3-G185	IC₅₀	34.3 μM Compound: Dipyridamole	TP_TRANSPORTER: inhibition of Rhodamine 123 efflux in NIH-3T3-G185 cells TP_TRANSPORTER: inhibition of Rhodamine 123 efflux in NIH-3T3-G185 cells	[PMID: 11716514]
Sf21	IC₅₀	72 μM Compound: 3	Inhibition of His-tagged catalytic domain Trypanosoma brucei brucei PDEB1 expressed in baculovirus infected insect Sf21 cells Inhibition of His-tagged catalytic domain Trypanosoma brucei brucei PDEB1 expressed in baculovirus infected insect Sf21 cells	[PMID: 22023548]
U2OS	IC₅₀	8.1 nM Compound: 3108	Inhibition of the equilibrative nucleoside transporter (ENT1, SLC29A1) as assessed by GPCR-mediated changes in cell morphology using the impedance-based transporter activity through receptor activation (TRACT) assay (PubChem AID: 1745861) Inhibition of the equilibrative nucleoside transporter (ENT1, SLC29A1) as assessed by GPCR-mediated changes in cell morphology using the impedance-based transporter activity through receptor activation (TRACT) assay (PubChem AID: 1745861)	[PMID: 31551431]

In Vitro

Dipyridamole (5 μM; 15 min) results in a 2.5-fold increase in intracellular cAMP levels in OCI-AML-3 cells^[2].
Dipyridamole (5 μM; 48 h) with the statin combination induces apoptosis in primary AML cells^[2].
Dipyridamole (5 μM; 48 h) possesse cAMP/PKA-independent activity against statininduced SREBP2 activation^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Apoptosis Analysis^[2]

Cell Line:	AML (OCI-AML-2, OCI-AML-3) cell line
Concentration:	5 μM
Incubation Time:	48 h
Result:	Induced apoptosis with the combination of fluvastatin and dipyridamole, cilostazol, forskolin, or dbcAMP in OCI-AML-2 and OCI-AML-3 cells.

RT-PCR^[2]

Cell Line:	LP1 cell line
Concentration:	5 μM
Incubation Time:	16 h
Result:	Increased the sensibility of cancer cells to statin-induced apoptosis.

In Vivo

Dipyridamole (10 mg/kg; p.o. once daily for 18 d) mitigates tumor growth, ameliorated concurrent alterations in blood circulation and tumor tissues, and platelet infiltration in tumor tissues^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	C57BL/6-LLC tumor-bearing mice models^[3]
Dosage:	10 mg/kg
Administration:	Oral gavage; 10 mg/kg; once daily for 18 days
Result:	Mitigated tumor growth in tumor-bearing mice.

Clinical Trial

Molecular Weight

504.63

Formula

C₂₄H₄₀N₈O₄

CAS No.

58-32-2

Appearance

Solid

Color

Light yellow to yellow

SMILES

OCCN(CCO)C1=NC(N2CCCCC2)=C(N=C(N(CCO)CCO)N=C3N4CCCCC4)C3=N1

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, protect from light

*In solvent : -80°C, 1 year; -20°C, 6 months (protect from light)

Solvent & Solubility

In Vitro:

DMSO : ≥ 50 mg/mL (99.08 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H₂O : < 0.1 mg/mL (insoluble)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	1.9816 mL	9.9082 mL	19.8165 mL
5 mM	0.3963 mL	1.9816 mL	3.9633 mL
10 mM	0.1982 mL	0.9908 mL	1.9816 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months (protect from light). When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (4.95 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (4.95 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (4.95 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.
Protocol 4

Add each solvent one by one: 2% DMSO 40% PEG300 5% Tween-80 53% Saline
Solubility: ≥ 1 mg/mL (1.98 mM); Clear solution
Protocol 5

Add each solvent one by one: 2% DMSO 98% Corn Oil
Solubility: 1 mg/mL (1.98 mM); Suspended solution; Need ultrasonic

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 1 year; -20°C, 6 months (protect from light)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 99.93%

Select Batch:

Data Sheet (284 KB) SDS (393 KB)

COA (257 KB) HNMR (275 KB) RP-HPLC (222 KB) LCMS (101 KB)

Handling Instructions (2659 KB)

References

[1]. Kerndt CC, Nagalli S. Dipyridamole. 2021 Nov 25. In: StatPearls [Internet]. Treasure Island (FL): StatPearls Publishing; 2022 Jan–. PMID: 32119342. [Content Brief]

[2]. Longo, Joseph, etal. Cyclic AMP-hydrolyzing phosphodiesterase inhibitors potentiate statin-induced cancer cell death. Molecular oncology vol. 14,10 (2020): 2533-2545 [Content Brief]

[3]. Wang, Jiaan-Der, etal. Exosomal HMGB1 Promoted Cancer Malignancy. Cancers vol. 13,4 877. 19 Feb. 2021. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	1.9816 mL	9.9082 mL	19.8165 mL	49.5412 mL
	5 mM	0.3963 mL	1.9816 mL	3.9633 mL	9.9082 mL
	10 mM	0.1982 mL	0.9908 mL	1.9816 mL	4.9541 mL
	15 mM	0.1321 mL	0.6605 mL	1.3211 mL	3.3027 mL
	20 mM	0.0991 mL	0.4954 mL	0.9908 mL	2.4771 mL
	25 mM	0.0793 mL	0.3963 mL	0.7927 mL	1.9816 mL
	30 mM	0.0661 mL	0.3303 mL	0.6605 mL	1.6514 mL
	40 mM	0.0495 mL	0.2477 mL	0.4954 mL	1.2385 mL
	50 mM	0.0396 mL	0.1982 mL	0.3963 mL	0.9908 mL
	60 mM	0.0330 mL	0.1651 mL	0.3303 mL	0.8257 mL
	80 mM	0.0248 mL	0.1239 mL	0.2477 mL	0.6193 mL

Dipyridamole Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Dipyridamole58-32-2Phosphodiesterase (PDE)Apoptosisantiplatelet phosphodiesterase (PDE) inhibitorcancerInhibitorinhibitorinhibit

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Dipyridamole

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Other Forms of Dipyridamole:

Dipyridamole Related Antibodies

11 Publications Citing Use of MCE Dipyridamole

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•Related Small Molecules:

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View All Phosphodiesterase (PDE) Isoform Specific Products:

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References

Customer Review

Dipyridamole Related Antibodies

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Complete Stock Solution Preparation Table

Dipyridamole Related Classifications

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