1. Immunology/Inflammation
    NF-κB
    Metabolic Enzyme/Protease
  2. COX
    Reactive Oxygen Species
  3. Hexahydrocurcumin

Hexahydrocurcumin 

Cat. No.: HY-N0929 Purity: 99.70%
Handling Instructions

Hexahydrocurcumin is one of the major metabolites of curcumin and a selective, orally active COX-2 inhibitor. Hexahydrocurcumin is inactive against COX-1. Hexahydrocurcumin has antioxidant, anticancer and anti-inflammatory activities.

For research use only. We do not sell to patients.

Hexahydrocurcumin Chemical Structure

Hexahydrocurcumin Chemical Structure

CAS No. : 36062-05-2

Size Price Stock Quantity
Solution
10 mM * 1 mL in DMSO USD 528 In-stock
Estimated Time of Arrival: December 31
Solid + Solvent
10 mM * 1 mL
ready for reconstitution
USD 528 In-stock
Estimated Time of Arrival: December 31
Solid
5 mg USD 480 In-stock
Estimated Time of Arrival: December 31
10 mg   Get quote  
50 mg   Get quote  

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Description

Hexahydrocurcumin is one of the major metabolites of curcumin and a selective, orally active COX-2 inhibitor. Hexahydrocurcumin is inactive against COX-1. Hexahydrocurcumin has antioxidant, anticancer and anti-inflammatory activities[1][2].

IC50 & Target[1]

COX-2

 

In Vitro

Hexahydrocurcumin (0-25 μM; 24-48 hours; HT-29 cells) treatment significantly decreased the viability of HT-29 colon cancer cells in a time- and concentration-dependent. The respective IC50 values for 24 and 48 h of Hexahydrocurcumin exposureare 77.05 and 56.95, respectively[1].
Hexahydrocurcumin (0-25 μM; 24-48 hours; HT-29 cells) combined with 5-fluorouracil (5-FU; 5 µM) markedly reduces the COX-2 expression. The level of COX-1 is not altered[1].
Hexahydrocurcumin (0-25 μM; 24-48 hours; HT-29 cells) combined with 5-fluorouracil (5-FU; 5 µM) markedly reduces the COX-2 protein. The level of COX-1 protein is not altered[1].
Hexahydrocurcumin (7-14 μM; 24 hours) attenuates lipopolysaccharide (LPS)-elicited increase of prostaglandin E2 (PGE2) in murine macrophages (RAW 264.7) in a concentration-dependent manner[2].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay[1]

Cell Line: HT-29 cells
Concentration: 0 µM, 5 µM, 10 µM, 25 µM
Incubation Time: 24 hours or 48 hours
Result: Significantly decreased the viability of HT-29 colon cancer cells.

RT-PCR[1]

Cell Line: HT-29 cells
Concentration: 25 µM
Incubation Time: 24 hours
Result: Combined with 5-fluorouracil (5-FU; 5 µM) markedly reduced the COX-2 expression.

Western Blot Analysis[1]

Cell Line: HT-29 cells
Concentration: 25 µM
Incubation Time: 24 hours
Result: Combined with 5-fluorouracil (5-FU; 5 µM) markedly reduced the COX-2 protein.
In Vivo

Hexahydrocurcumin (50 mg/kg; oral administration; daily; for 16 weeks; male Wistar rats) treatment significantly reduces the numbers of aberrant crypt foci (ACF) in colon cancer rats. Hexahydrocurcumin also markedly decreases COX-2 protein expression. The levels of COX-1 protein is not different from normal rats[3].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Male Wistar rats (100-120 g) injected with dimethylhydrazine (DMH)[3]
Dosage: 50 mg/kg
Administration: Oral administration; daily; for 16 weeks
Result: Significantly reduced the numbers of ACF in colon cancer rats. Also markedly decreased COX-2 protein expression.
Molecular Weight

374.43

Formula

C₂₁H₂₆O₆

CAS No.
Shipping

Room temperature in continental US; may vary elsewhere.

Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : 100 mg/mL (267.07 mM; Need ultrasonic)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.6707 mL 13.3536 mL 26.7073 mL
5 mM 0.5341 mL 2.6707 mL 5.3415 mL
10 mM 0.2671 mL 1.3354 mL 2.6707 mL
*Please refer to the solubility information to select the appropriate solvent.
In Vivo:
  • 1.

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% saline

    Solubility: ≥ 2.5 mg/mL (6.68 mM); Clear solution

  • 2.

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in saline)

    Solubility: ≥ 2.5 mg/mL (6.68 mM); Clear solution

  • 3.

    Add each solvent one by one:  10% DMSO    90% corn oil

    Solubility: ≥ 2.5 mg/mL (6.68 mM); Clear solution

*All of the co-solvents are provided by MCE.
References
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Hexahydrocurcumin
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