1. Immunology/Inflammation
  2. COX
  3. Isoorientin

Isoorientin (Synonyms: Homoorientin)

Cat. No.: HY-N0767 Purity: 99.26%
Handling Instructions

Isoorientin is a potent inhibitor of COX-2 with an IC50 value of 39 μM.

For research use only. We do not sell to patients.

Isoorientin Chemical Structure

Isoorientin Chemical Structure

CAS No. : 4261-42-1

Size Price Stock Quantity
10 mM * 1 mL in DMSO USD 118 In-stock
Estimated Time of Arrival: December 31
1 mg USD 60 In-stock
Estimated Time of Arrival: December 31
5 mg USD 120 In-stock
Estimated Time of Arrival: December 31
10 mg USD 192 In-stock
Estimated Time of Arrival: December 31
25 mg USD 396 In-stock
Estimated Time of Arrival: December 31
50 mg USD 672 In-stock
Estimated Time of Arrival: December 31
100 mg   Get quote  
200 mg   Get quote  

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Based on 2 publication(s) in Google Scholar

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Description

Isoorientin is a potent inhibitor of COX-2 with an IC50 value of 39 μM.

IC50 & Target[1]

COX-2

39 μM (IC50)

In Vitro

Isoorientin is a Selective Inhibitor of Cyclooxygenase-2 (COX-2) from the Tubers of Pueraria tuberosa[1]. PANC-1 and PATU-8988 cells are grown for 24 hours in the presence of Isoorientin (0, 20, 40, 80, and 160 μM), and a CCK8 solution is added. The cell viability decreases significantly at the concentrations of 20, 40, 80, and 160 μM. After the cells are cultured with Isoorientin (0, 20, 40, 80, and 160 μM for PANC-1; 0, 20, 40, 80, 160, and 320 μM for PATU-8988) for 24 hours, the expression of p-AMPK and AMPK is assessed by Western blotting. After the Isoorientin treatment, the p-AMPK expression is increased. Then, in the shRNA group, the concentration of 80 μM is used to detect the effects of Isoorientin. The expression levels of AMPK and p-AMPK are much lower in the shRNA group than in the wild-type PC cells (WT) and the group that is transfected with a negative control lentivirus (NC)[2].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

Animals treated with Isoorientin at 10 mg/kg and 20 mg/kg body weight have a statistically significant reduction in paw edema, with a mean peak thickness of 1.19±0.05 mm and 1.08±0.04 mm, respectively. This indicated that Isoorientin significantly attenuates paw edema compared with the control group[3].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

448.38

Formula

C₂₁H₂₀O₁₁

CAS No.

4261-42-1

SMILES

OC1=C(C2=O)C(OC(C3=CC(O)=C(O)C=C3)=C2)=CC(O)=C1[[email protected]@H]([[email protected]@H]([[email protected]@H](O)[[email protected]@H]4O)O)O[[email protected]@H]4CO

Shipping

Room temperature in continental US; may vary elsewhere.

Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : ≥ 100 mg/mL (223.03 mM)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.2303 mL 11.1513 mL 22.3025 mL
5 mM 0.4461 mL 2.2303 mL 4.4605 mL
10 mM 0.2230 mL 1.1151 mL 2.2303 mL
*Please refer to the solubility information to select the appropriate solvent.
In Vivo:
  • 1.

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% saline

    Solubility: ≥ 2.5 mg/mL (5.58 mM); Clear solution

  • 2.

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in saline)

    Solubility: ≥ 2.5 mg/mL (5.58 mM); Clear solution

*All of the co-solvents are provided by MCE.
References
Cell Assay
[2]

PANC-1 and PATU-8988 cells are plated onto 96-well plates. Each well contain ~5,000 cells and 200 μL of the medium with 10% FBS. When the cells of each well reach 70% confluency, the medium is changed, and FBS-free medium with different concentrations of Isoorientin is added. After 24 hours, the cells are washed with PBS once, the medium containing Isoorientin is discarded, and 100 μL of FBS-free medium with 10 μL of the Cell Counting Kit 8 (CCK8) reagent are added. The cells are incubated for another 1-2 hours at 37°C, and the absorbance of each well is detected using an ELISA reader at 490 nm. Cell viability is expressed as the fold change of absorbance[2].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Administration
[3]

Mice[3]
In the case of paw edema model the Isoorientin or Celecoxib is given intraperitoneally and Carrageenan is injected into the paw directly one hour later. In air pouch model all the treatments are given along with Carrageenan directly into the pouch cavity. Isoorientin is injected three hours earlier than injection of Carrageenan into the pouch cavity. Isoorientin and Celecoxib are administered into the mice air pouch. The stock solutions of Isoorientin (100 mg/mL) and Celecoxib (100 mg/mL) are prepared in DMSO and further dilutions are made at the time of treatments. Animals are divided, into 5 different groups as follows: control (DMSO treated); Carrageenan (0.5 mL of 1.5% (w/v) Carrageenan in saline) treated; Carrageenan+Celecoxib (20 mg/kg body weight) treated; Carrageenan+Isoorientin (10 mg/Kg body weight) treated; Carrageenan+Isoorientin (20 mg/Kg body weight) treated.

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

References
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Keywords:

IsoorientinHomoorientinCOXCyclooxygenaseInhibitorinhibitorinhibit

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