Isoorientin
Based on 8 publication(s) in Google Scholar
Isoorientin is a potent inhibitor of COX-2 with an IC50 value of 39 μM.
For research use only. We do not sell to patients.
- Purity: 98.36%
- CAS No.: 4261-42-1
- Formula: C21H20O11
- Molecular Weight:448.38
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Storage:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Protocol
- Purity & Documentation
- References
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Help & FAQs
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Publications Citing Use of MedChemExpress (MCE) Isoorientin
More- J Adv Res. 2025 Aug:74:651-666. [Abstract]
- Acta Pharm Sin B. 2021 Jan;11(1):143-155. [Abstract]
- Mol Med. 2024 Feb 20;30(1):27. [Abstract]
- Eur J Med Chem. 2020 Dec 15:208:112754. [Abstract]
- Pharmaceuticals (Basel). 2022 Dec 12;15(12):1541. [Abstract]
- Int Immunopharmacol. 2026 Apr 1:174:116299. [Abstract]
- Animals (Basel). 2024 Sep 28;14(19):2806. [Abstract]
- Biochem Biophys Res Commun. 2022 Apr 2:598:81-88. [Abstract]
Customer Validation & Images
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Cell Proliferation/Viability Assay
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IF
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WB
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Histological Imaging/Staining
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Cell Imaging/Staining
Biological Activity
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COX-2 39 μM (IC50) |
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Cell Line
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Type | Value | Description | References |
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| Huh-7 | CC50 |
>50 μM
Compound: 11
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Cytotoxicity against human Huh7.5.1 cells by MTT assay
Cytotoxicity against human Huh7.5.1 cells by MTT assay
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[PMID: 22445328] |
| SH-SY5Y | EC50 |
47 μM
Compound: 80
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Neuroprotective activity against human SH-SY5Y cells pretreated for 1 hr followed by muMAbeta42 measured after 72 hrs
Neuroprotective activity against human SH-SY5Y cells pretreated for 1 hr followed by muMAbeta42 measured after 72 hrs
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[PMID: 35390715] |
Isoorientin is a Selective Inhibitor of Cyclooxygenase-2 (COX-2) from the Tubers of Pueraria tuberosa[1]. PANC-1 and PATU-8988 cells are grown for 24 hours in the presence of Isoorientin (0, 20, 40, 80, and 160 μM), and a CCK8 solution is added. The cell viability decreases significantly at the concentrations of 20, 40, 80, and 160 μM. After the cells are cultured with Isoorientin (0, 20, 40, 80, and 160 μM for PANC-1; 0, 20, 40, 80, 160, and 320 μM for PATU-8988) for 24 hours, the expression of p-AMPK and AMPK is assessed by Western blotting. After the Isoorientin treatment, the p-AMPK expression is increased. Then, in the shRNA group, the concentration of 80 μM is used to detect the effects of Isoorientin. The expression levels of AMPK and p-AMPK are much lower in the shRNA group than in the wild-type PC cells (WT) and the group that is transfected with a negative control lentivirus (NC)[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 4261-42-1
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Appearance Solid
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Molecular Weight 448.38
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Formula C21H20O11
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Color Light yellow to yellow
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SMILES
OC1=C(C2=O)C(OC(C3=CC(O)=C(O)C=C3)=C2)=CC(O)=C1[C@@H]([C@@H]([C@@H](O)[C@@H]4O)O)O[C@@H]4CO
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Synonyms
Homoorientin
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Structure Classification
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Initial Source
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Publications (8)
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Journal Impact Factor
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Most Recent
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J Adv Res
A multi-omics approach reveals that lotus root polysaccharide iron ameliorates iron deficiency-induced testicular damage by activating PPARγ to promote steroid hormone synthesis. [Abstract]2025 Aug:74:651-666. PMID: 39343163 -
Acta Pharm Sin B
Chrysin serves as a novel inhibitor of DGK α/FAK interaction to suppress the malignancy of esophageal squamous cell carcinoma (ESCC). [Abstract]2021 Jan;11(1):143-155. PMID: 33532186 -
Mol Med
Exploring the therapeutic potential of isoorientin in the treatment of osteoporosis: a study using network pharmacology and experimental validation. [Abstract]2024 Feb 20;30(1):27. PMID: 38378457
Isoorientin purchased from MedChemExpress. Usage Cited in: Mol Med. 2024 Feb 20;30(1):27. [Abstract]
RAW264.7 cell viability as assessed by CCK-8 assay following treatment with or without indicated concentrations of Isoorientin (ISO) (0, 20, 40, 50, 80, 100 μM) for 48 and 96 h.
Isoorientin purchased from MedChemExpress. Usage Cited in: Mol Med. 2024 Feb 20;30(1):27. [Abstract]
Representative fluorescence images of actin stained RAW264.7 cells stimulated with RANKL without or with different concentrations of Isoorientin (ISO) (0, 20, 40, 60, 100 μM).
Isoorientin purchased from MedChemExpress. Usage Cited in: Mol Med. 2024 Feb 20;30(1):27. [Abstract]
RAW264.7 cells were stimulated with RANKL for the indicated times in the presence or absence of Isoorientin (ISO) at 100 μM respectively, and analyzed by western blot using specific antibodies against c-Fos, NFATc1, and β-actin.
Isoorientin purchased from MedChemExpress. Usage Cited in: Mol Med. 2024 Feb 20;30(1):27. [Abstract]
Representative image of H&E staining. The OVX group showed significant bone destruction, while Isoorientin (ISO) (15 mg/kg, i.p.) treatment significantly ameliorated OVX-induced bone loss.
Isoorientin purchased from MedChemExpress. Usage Cited in: Mol Med. 2024 Feb 20;30(1):27. [Abstract]
RANKL-stimulated RAW264.7 cells treated with or without the indicated concentrations of Isoorientin (ISO) (0, 20, 40, 60, 100 μM) were stained with TRAP, and representative images were shown.
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Eur J Med Chem
Unraveling the anti-influenza effect of flavonoids: Experimental validation of luteolin and its congeners as potent influenza endonuclease inhibitors. [Abstract]2020 Dec 15:208:112754. PMID: 32883638 -
Pharmaceuticals (Basel)
A Mechanism of Isoorientin-Induced Apoptosis and Migration Inhibition in Gastric Cancer AGS Cells. [Abstract]2022 Dec 12;15(12):1541. PMID: 36558992 -
Int Immunopharmacol
The mechanism study of isoorientin regulating neuroinflammation after subarachnoid hemorrhage through AKT/GSK3β. [Abstract]2026 Apr 1:174:116299. PMID: 41671619 -
Animals (Basel)
Isoorientin Promotes Early Porcine Embryonic Development by Alleviating Oxidative Stress and Improving Lipid Metabolism. [Abstract]2024 Sep 28;14(19):2806. PMID: 39409754 -
Biochem Biophys Res Commun
2022 Apr 2:598:81-88. PMID: 35151208
Solvent & Solubility
DMSO : ≥ 100 mg/mL (223.03 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
* "≥" means soluble, but saturation unknown.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (5.58 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (5.58 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Protocol
PANC-1 and PATU-8988 cells are plated onto 96-well plates. Each well contain ~5,000 cells and 200 μL of the medium with 10% FBS. When the cells of each well reach 70% confluency, the medium is changed, and FBS-free medium with different concentrations of Isoorientin is added. After 24 hours, the cells are washed with PBS once, the medium containing Isoorientin is discarded, and 100 μL of FBS-free medium with 10 μL of the Cell Counting Kit 8 (CCK8) reagent are added. The cells are incubated for another 1-2 hours at 37°C, and the absorbance of each well is detected using an ELISA reader at 490 nm. Cell viability is expressed as the fold change of absorbance[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Mice[3]
In the case of paw edema model the Isoorientin or Celecoxib is given intraperitoneally and Carrageenan is injected into the paw directly one hour later. In air pouch model all the treatments are given along with Carrageenan directly into the pouch cavity. Isoorientin is injected three hours earlier than injection of Carrageenan into the pouch cavity. Isoorientin and Celecoxib are administered into the mice air pouch. The stock solutions of Isoorientin (100?mg/mL) and Celecoxib (100?mg/mL) are prepared in DMSO and further dilutions are made at the time of treatments. Animals are divided, into 5 different groups as follows: control (DMSO treated); Carrageenan (0.5?mL of 1.5% (w/v) Carrageenan in saline) treated; Carrageenan+Celecoxib (20?mg/kg body weight) treated; Carrageenan+Isoorientin (10?mg/Kg body weight) treated; Carrageenan+Isoorientin (20?mg/Kg body weight) treated.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Purity & Documentation
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Data Sheet (282 KB)
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SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
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Handling Instructions (2659 KB)
References
[1]. Sumalatha M, et al. Isoorientin, a Selective Inhibitor of Cyclooxygenase-2 (COX-2) from the Tubers of Pueraria tuberosa. Nat Prod Commun. 2015 Oct;10(10):1703-4. [Content Brief]
[2]. Ye T, et al. Isoorientin induces apoptosis, decreases invasiveness, and downregulates VEGF secretion by activating AMPK signaling in pancreatic cancer cells. Onco Targets Ther. 2016 Dec 12;9:7481-7492. [Content Brief]
[3]. Anilkumar K, et al. Evaluation of Anti-Inflammatory Properties of Isoorientin Isolated from Tubers of Pueraria tuberosa. Oxid Med Cell Longev. 2017;2017:5498054. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
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| DMSO | 1 mM | 2.2303 mL | 11.1513 mL | 22.3025 mL | 55.7563 mL |
| 5 mM | 0.4461 mL | 2.2303 mL | 4.4605 mL | 11.1513 mL | |
| 10 mM | 0.2230 mL | 1.1151 mL | 2.2303 mL | 5.5756 mL | |
| 15 mM | 0.1487 mL | 0.7434 mL | 1.4868 mL | 3.7171 mL | |
| 20 mM | 0.1115 mL | 0.5576 mL | 1.1151 mL | 2.7878 mL | |
| 25 mM | 0.0892 mL | 0.4461 mL | 0.8921 mL | 2.2303 mL | |
| 30 mM | 0.0743 mL | 0.3717 mL | 0.7434 mL | 1.8585 mL | |
| 40 mM | 0.0558 mL | 0.2788 mL | 0.5576 mL | 1.3939 mL | |
| 50 mM | 0.0446 mL | 0.2230 mL | 0.4461 mL | 1.1151 mL | |
| 60 mM | 0.0372 mL | 0.1859 mL | 0.3717 mL | 0.9293 mL | |
| 80 mM | 0.0279 mL | 0.1394 mL | 0.2788 mL | 0.6970 mL | |
| 100 mM | 0.0223 mL | 0.1115 mL | 0.2230 mL | 0.5576 mL |
Isoorientin Related Classifications
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