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  4. Rhodamine 6G

Rhodamine dyes are membrane-permeable cationic fluorescent probes that specifically recognize mitochondrial membrane potentials, thereby attaching to mitochondria and producing bright fluorescence, and at certain concentrations, rhodamine dyes have low toxicity to cells, so they are commonly used to detect mitochondria in animal cells, plant cells, and microorganisms.

For research use only. We do not sell to patients.

Rhodamine 6G

Rhodamine 6G Chemical Structure

CAS No. : 989-38-8

Size Price Stock Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
 In-stock
Solution
10 mM * 1 mL in DMSO In-stock
Solid
50 mg In-stock
100 mg In-stock
250 mg In-stock
1 g In-stock
5 g In-stock
10 g In-stock
50 g   Get quote  

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Customer Review

Based on 4 publication(s) in Google Scholar

Other Forms of Rhodamine 6G:

Rhodamine 6G Related Antibodies

Top Publications Citing Use of Products

  • Biological Activity

  • Protocol

  • Purity & Documentation

  • References

  • Customer Review

Description

Rhodamine dyes are membrane-permeable cationic fluorescent probes that specifically recognize mitochondrial membrane potentials, thereby attaching to mitochondria and producing bright fluorescence, and at certain concentrations, rhodamine dyes have low toxicity to cells, so they are commonly used to detect mitochondria in animal cells, plant cells, and microorganisms[1].

In Vitro

Guide (The following is our recommended experimental protocol. This protocol serves merely as a reference guide; specific procedures should be adjusted according to your actual requirements.)
1. Preparation of Rhodamine 6G Working Solution
1.1 Preparation of Stock Solution
Dilute 1 mg of Rhodamine 6G with 525 μL of anhydrous DMSO to prepare a 5 mM stock solution.
1.2 Preparation of Working Solution
Dilute the stock solution with pre-warmed serum-free cell culture medium or PBS to prepare a Rhodamine 6G working solution with a concentration of 1–20 μM.
Note: Please adjust the concentration of the Rhodamine 6G working solution according to your specific needs, and prepare it immediately before use.
2. Cell Staining (Suspension Cells)
2.1 Centrifuge to harvest the cells, then wash twice with PBS (5 minutes per wash). Adjust the cell density to 1 × 106 cells/mL.
2.2 Add 1 mL of the Rhodamine 6G working solution and incubate at room temperature for 30–60 minutes.
2.3 Centrifuge at 400 × g for 3–4 minutes, and discard the supernatant.
2.4 Wash the cells twice with PBS (5 minutes per wash).
2.5 Resuspend the cells in 1 mL of serum-free medium or PBS, then observe using a fluorescence microscope or flow cytometer.
3. Cell Staining (Adherent Cells)
3.1 Culture the adherent cells on sterile coverslips.
3.2 Remove the coverslips from the culture medium and aspirate any excess medium.
3.3 Add 100 μL of the dye working solution, gently agitate to ensure the solution completely covers the cells, and incubate for 5–30 minutes.
3.4 Aspirate the working dye solution, wash with culture medium 2–3 times (5 minutes each), and observe using a fluorescence microscope or flow cytometer.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Rhodamine 6G Related Antibodies
In Vivo

Melanoma-transplanted mice receiving Rhodamine 6G demonstrate prolonged survival, improved clinical parameters, inhibited tumor growth and metastases count, compared to their untreated counterparts. Twice-a-week 10-6M Rhodamine 6G regimen yield the most prominent results[2]. The Rhodamine-6G enters the circulatory system and labels leukocytes. It is possible to monitor changes in the interactions between leukocytes and the endothelium by determining the numbers of rolling and adhering leukocytes as well as the total flux of these cells[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Clinical Trial
Molecular Weight

479.01

Formula

C28H31ClN2O3
CAS No.
Appearance

Solid

Color

Brown to reddish brown

Emission (Em)

552
Excitation (Ex)

530
SMILES

CC1=CC2=C(C3=CC=CC=C3C(OCC)=O)C4=C([O+]=C2C=C1NCC)C=C(NCC)C(C)=C4.[Cl-]
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, sealed storage, away from moisture and light

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

Solvent & Solubility
In Vitro: 

DMSO : 23.33 mg/mL (48.70 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H2O : 10 mg/mL (20.88 mM; ultrasonic and warming and heat to 60°C)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.0876 mL 10.4382 mL 20.8764 mL
5 mM 0.4175 mL 2.0876 mL 4.1753 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
Purity & Documentation

Purity: 98.02%

SDS (785 KB)

COA (252 KB) HNMR (272 KB) LCMS (333 KB)

Handling Instructions (2659 KB)
References
Cell Assay
[2]

Malignant cells and normal control cultures are seeded in equal (protein adjusted) cell amounts into 6-well tissue culture plates. The cells are pulsed with 25µCi/mL of 3H-Thymidine and immediately treated with Rhodamine 6G at the fixed concentration of 1 μM for 24h, 48h, 72h or 5 days (120h). Following 24h, 48h, 72h or 5 days, the excessive radioactive material is ished out with PBS. The cell samples are transferred into polystyrene vials containing 4 ml scintillation liquid, and their radioactivity counted in a β-counter. Total cell protein is assessed by Bradford’s assay[2].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
Animal Administration
[2]

Mice: C57Bl mice are implanted with B16-F10 melanoma and treated with Rhodamine 6G (1, 0.1, 0.01 μM) at different dosage/time regimens. Viability and proliferation of cultured tumor cells are analyzed[2].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
H2O / DMSO 1 mM 2.0876 mL 10.4382 mL 20.8764 mL 52.1910 mL
5 mM 0.4175 mL 2.0876 mL 4.1753 mL 10.4382 mL
10 mM 0.2088 mL 1.0438 mL 2.0876 mL 5.2191 mL
15 mM 0.1392 mL 0.6959 mL 1.3918 mL 3.4794 mL
20 mM 0.1044 mL 0.5219 mL 1.0438 mL 2.6095 mL
DMSO 25 mM 0.0835 mL 0.4175 mL 0.8351 mL 2.0876 mL
30 mM 0.0696 mL 0.3479 mL 0.6959 mL 1.7397 mL
40 mM 0.0522 mL 0.2610 mL 0.5219 mL 1.3048 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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Rhodamine 6G Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Rhodamine 6G989-38-8Basic Red 1Basic Red1Basic Red-1Fluorescent DyeEnvironmental PollutantsEnvironmental ContaminantsInhibitorinhibitorinhibit

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