Cat. No.: HY-10882

Clotrimazole

9 Cited Publications

Customer Review

Based on 9 publication(s) in Google Scholar

Clotrimazole is an imidazole derivative, an antifungal compound and is a CYP (cytochrome P450) inhibitor. Clotrimazole has antibacterial activity.

For research use only. We do not sell to patients.

Purity: 99.91%
CAS No.: 23593-75-1
Formula: C₂₂H₁₇ClN₂
Molecular Weight:344.84

Storage:

Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 2 years , -20°C, 1 year

Clotrimazole Chemical Structure

CAS No.: 23593-75-1

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Top Publications Citing

Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- IC₅₀ & Target
- Cellular Effect
- In Vitro
- Clinical Trial
Chemical Information
Publications (9)
Solvent & Solubility
- In Vitro
- In Vivo
Purity & Documentation
References
Help & FAQs
Classifications
Bioz

Biological Activity

Description

Clotrimazole is an imidazole derivative, an antifungal compound and is a CYP (cytochrome P450) inhibitor. Clotrimazole has antibacterial activity.

IC₅₀ & Target

Antifungal; CYP

All Antibiotic Isoforms :

Cellular Effect

Cell Line	Type	Value	Description	References
A549	IC₅₀	5.1 μM Compound: 1, CLT	Antiproliferative activity against human A549 cells by SRB assay Antiproliferative activity against human A549 cells by SRB assay	[PMID: 19027297]
COS-7	IC₅₀	0.07 μM Compound: Clotrimazole	Inhibition of human cloned IK1 expressed in african green monkey COS7 cells by whole cell patch clamp assay Inhibition of human cloned IK1 expressed in african green monkey COS7 cells by whole cell patch clamp assay	[PMID: 19282171]
CV-1	IC₅₀	0.69 μM Compound: 2	Inverse agonist activity at human CAR-LBD transfected in CV-1 cells after 24 hrs by luciferase reporter gene assay Inverse agonist activity at human CAR-LBD transfected in CV-1 cells after 24 hrs by luciferase reporter gene assay	[PMID: 26717202]
HaCaT	EC₅₀	15 μM Compound: Clotrimazole	Inhibition of human HaCaT cell proliferation after 48 hrs Inhibition of human HaCaT cell proliferation after 48 hrs	[PMID: 19282171]
HEK293	EC₅₀	3 μM Compound: clotrimazole	Cytotoxicity against HEK293 cells Cytotoxicity against HEK293 cells	[PMID: 18973326]
HEK293	IC₅₀	0.59 μM Compound: Clotrimazole	Inhibition of L-type calcium channel measured using 2-electrode voltage-clamp in human embryonic kidney cells heterologically expressing alpha-1C subunit Inhibition of L-type calcium channel measured using 2-electrode voltage-clamp in human embryonic kidney cells heterologically expressing alpha-1C subunit	[PMID: 22761000]
HEK293	IC₅₀	11.97 μM Compound: Clotrimazole	Inhibition of human OCT1 expressed in HEK293 cells assessed as decrease in uptake of ASP+ after 2 mins by fluorescence assay Inhibition of human OCT1 expressed in HEK293 cells assessed as decrease in uptake of ASP+ after 2 mins by fluorescence assay	[PMID: 28230985]
HEK-293T	CC₅₀	7 μg/mL Compound: Clotrimazole	Cytotoxicity against HEK293T cells assessed as reduction in cell viability measured after 24 hrs by MTT assay Cytotoxicity against HEK293T cells assessed as reduction in cell viability measured after 24 hrs by MTT assay	[PMID: 27720324]
HEK-293T	IC₅₀	5.9 μM Compound: Clotrimazole	Inhibition of mouse Ido2 transfected in HEK293T cells using L-tryptophan as substrate assessed as kynurenine formation after 45 mins by spectrophotometric analysis Inhibition of mouse Ido2 transfected in HEK293T cells using L-tryptophan as substrate assessed as kynurenine formation after 45 mins by spectrophotometric analysis	[PMID: 23122865]
HEK-293T	IC₅₀	51.6 μM Compound: Clotrimazole	Inhibition of mouse Ido1 transfected in HEK293T cells using L-tryptophan as substrate assessed as kynurenine formation after 45 mins by spectrophotometric analysis Inhibition of mouse Ido1 transfected in HEK293T cells using L-tryptophan as substrate assessed as kynurenine formation after 45 mins by spectrophotometric analysis	[PMID: 23122865]
HeLa	IC₅₀	1.4 μM Compound: Clotrimazole	Inhibition of p97 in human HeLa cells assessed as reduction in p97-dependent UbG76V-GFP degradation incubated for 1 hr by luciferase reporter gene assay Inhibition of p97 in human HeLa cells assessed as reduction in p97-dependent UbG76V-GFP degradation incubated for 1 hr by luciferase reporter gene assay	[PMID: 31550150]
HEp-2	CC₅₀	18 μg/mL Compound: Clotrimazole	Cytotoxicity against human Hep2 cells assessed as reduction in cell viability after 24 hrs by trypan blue exclusion assay Cytotoxicity against human Hep2 cells assessed as reduction in cell viability after 24 hrs by trypan blue exclusion assay	[PMID: 27100030]
HEp-2	CC₅₀	38 μg/mL Compound: C	Cytotoxicity against human Hep2 cells assessed as reduction of cell viability after 24 hrs by trypan blue exclusion assay Cytotoxicity against human Hep2 cells assessed as reduction of cell viability after 24 hrs by trypan blue exclusion assay	[PMID: 23702472]
HEp-2	CC₅₀	38 μg/mL Compound: Clotrimazole	Cytotoxicity against human Hep2 cells assessed as cell survival after 24 hrs by trypan blue exclusion method Cytotoxicity against human Hep2 cells assessed as cell survival after 24 hrs by trypan blue exclusion method	[PMID: 26562544]
HEp-2	CC₅₀	38.8 μg/mL Compound: Clotrimazole	Cytotoxicity against human Hep2 cells assessed as reduction in cell viability incubated for 24 hrs by trypan blue dye exclusion assay Cytotoxicity against human Hep2 cells assessed as reduction in cell viability incubated for 24 hrs by trypan blue dye exclusion assay	[PMID: 28963991]
HEp-2	EC₅₀	40 μg/mL Compound: C	Cytotoxicity against human Hep2 cells assessed as cell survival fraction after 24 hrs by trypan blue exclusion method Cytotoxicity against human Hep2 cells assessed as cell survival fraction after 24 hrs by trypan blue exclusion method	[PMID: 22560629]
HEp-2	EC₅₀	48 μg/mL Compound: C	Antiproliferative activity against human Hep2 cells assessed as cell survival fraction after 24 hrs by MTT assay Antiproliferative activity against human Hep2 cells assessed as cell survival fraction after 24 hrs by MTT assay	[PMID: 22560629]
Huh-7	CC₅₀	25.8 μM Compound: GNF-Pf-3499	NOVARTIS: Cytotoxicity against human hepatocellular carcinoma cell line (Huh7) NOVARTIS: Cytotoxicity against human hepatocellular carcinoma cell line (Huh7)	[PMID: 18579783]
LLC-PK1	IC₅₀	3.5 μM Compound: Clotrimazole	Inhibition of P-glycoprotein, mouse L-mdr1b expressed in LLC-PK1 epithelial cells using calcein-AM polarisation assay Inhibition of P-glycoprotein, mouse L-mdr1b expressed in LLC-PK1 epithelial cells using calcein-AM polarisation assay	[PMID: 12699389]
LLC-PK1	IC₅₀	3.5 μM Compound: Clotrimazole	TP_TRANSPORTER: inhibition of Calcein-AM efflux in Mdr1b-expressing LLC-PK1 cells TP_TRANSPORTER: inhibition of Calcein-AM efflux in Mdr1b-expressing LLC-PK1 cells	[PMID: 12699389]
LLC-PK1	IC₅₀	4.8 μM Compound: Clotrimazole	Inhibition of P-glycoprotein, mouse L-mdr1a expressed in LLC-PK1 epithelial cells using calcein-AM polarisation assay Inhibition of P-glycoprotein, mouse L-mdr1a expressed in LLC-PK1 epithelial cells using calcein-AM polarisation assay	[PMID: 12699389]
LLC-PK1	IC₅₀	4.8 μM Compound: Clotrimazole	TP_TRANSPORTER: inhibition of Calcein-AM efflux in Mdr1a-expressing LLC-PK1 cells TP_TRANSPORTER: inhibition of Calcein-AM efflux in Mdr1a-expressing LLC-PK1 cells	[PMID: 12699389]
LLC-PK1	IC₅₀	6.7 μM Compound: Clotrimazole	Inhibition of P-glycoprotein, human L-MDR1 expressed in LLC-PK1 epithelial cells using calcein-AM polarisation assay Inhibition of P-glycoprotein, human L-MDR1 expressed in LLC-PK1 epithelial cells using calcein-AM polarisation assay	[PMID: 12699389]
LLC-PK1	IC₅₀	6.7 μM Compound: Clotrimazole	TP_TRANSPORTER: inhibition of Calcein-AM efflux in MDR1-expressing LLC-PK1 cells TP_TRANSPORTER: inhibition of Calcein-AM efflux in MDR1-expressing LLC-PK1 cells	[PMID: 12699389]
MCF7	CC₅₀	5 μg/mL Compound: Clotrimazole	Cytotoxicity against human MCF7 cells assessed as reduction in cell viability measured after 24 hrs by MTT assay Cytotoxicity against human MCF7 cells assessed as reduction in cell viability measured after 24 hrs by MTT assay	[PMID: 27720324]
NIH3T3	IC₅₀	0.63 μM Compound: 1 (CLT)	Mitogen-induced cell proliferation assay to evaluate the antiproliferative effect in NIH 3T3 cells Mitogen-induced cell proliferation assay to evaluate the antiproliferative effect in NIH 3T3 cells	[PMID: 14698156]
NIH3T3	IC₅₀	289.45 μg/mL Compound: Clotrimazole	Cytotoxicity against mouse NIH/3T3 cells assessed as reduction in cell viability after 24 hrs by MTT assay Cytotoxicity against mouse NIH/3T3 cells assessed as reduction in cell viability after 24 hrs by MTT assay	[PMID: 29274492]
Ventricular myocyte	IC₅₀	20 μM Compound: Clotrimazole	Inhibition of L-type calcium channel measured using whole-cell patch clamp in guinea pig ventricular myocytes Inhibition of L-type calcium channel measured using whole-cell patch clamp in guinea pig ventricular myocytes	[PMID: 22761000]
Vero	EC₅₀	10 M Compound: CTZ	Effective concentration to inhibit the proliferation of amastigote form of the parasite grown on mammalian (Vero) cells Effective concentration to inhibit the proliferation of amastigote form of the parasite grown on mammalian (Vero) cells	[PMID: 8336342]

In Vitro

Clotrimazole is an antifungal agent commonly used in the study of fungal infections such as vaginal yeast infections, oral thrush, and ringworm^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Clinical Trial

NCT Number	Sponsor	Condition	Start Date	Phase A549
NCT01329991	Plexxikon\|		2011-05	PHASE1

Chemical Information

CAS No. 23593-75-1
Appearance Solid
Molecular Weight 344.84
Formula C₂₂H₁₇ClN₂
Color White to off-white
SMILES

ClC1=CC=CC=C1C(N2C=CN=C2)(C3=CC=CC=C3)C4=CC=CC=C4
Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	2 years
	-20°C	1 year

Publications (9)

All Bio/Physico-chemical AssayIn Vivo Efficacy StudyHistological Imaging/StainingWB

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Journal Impact Factor

Adv Sci (Weinh)

Targeting Transient Receptor Potential Melastatin-2 (TRPM2) Enhances Therapeutic Efficacy of Third Generation EGFR Inhibitors against EGFR Mutant Lung Cancer. [Abstract]

2024 Jul 23:e2310126. PMID: 39044361
Nat Chem Biol

Persistent activation of TRPM4 triggers necrotic cell death characterized by sodium overload. [Abstract]

2025 Aug;21(8):1238-1249. PMID: 39915626
Phytomedicine

Cinobufagin overcomes bortezomib resistance in multiple myeloma strains by targeting SEC62/TRPM4-mediated NECSO. [Abstract]

2025 Aug 16:147:157171. PMID: 40839992
Sci Data

High-content, arrayed compound screens with rhinovirus, influenza A virus and herpes simplex virus infections. [Abstract]

2022 Oct 8;9(1):610. PMID: 36209289
Int J Mol Sci

A Functional Pipeline of Genome-Wide Association Data Leads to Midostaurin as a Repurposed Drug for Alzheimer's Disease. [Abstract]

2023 Jul 28;24(15):12079. PMID: 37569459

Clotrimazole purchased from MedChemExpress. Usage Cited in: Int J Mol Sci. 2023 Jul 28;24(15):12079. [Abstract]

Motility of GMC101 treated with Clotrimazole (10-30 h) at 0.1 μM.
Front Endocrinol

PFKP Activation Ameliorates Foot Process Fusion in Podocytes in Diabetic Kidney Disease. [Abstract]

2022 Jan 14;12:797025. PMID: 35095764

Clotrimazole purchased from MedChemExpress. Usage Cited in: Front Endocrinol. 2022 Jan 14;12:797025. [Abstract]

Survival curve of db/m and db/db mice treated with Clotrimazole (100 mg/kg, ip, 2 weeks) over time.

Clotrimazole purchased from MedChemExpress. Usage Cited in: Front Endocrinol. 2022 Jan 14;12:797025. [Abstract]

ACR was significantly higher in Clotrimazole (100 mg/kg, ip, 2 weeks)–administrated db/db mice when compared with solvent-treated db/db mice.

Clotrimazole purchased from MedChemExpress. Usage Cited in: Front Endocrinol. 2022 Jan 14;12:797025. [Abstract]

The mortality rate was not impacted by Clotrimazole (100 mg/kg, ip, 2 weeks) administration in db/m mice. In order to identify the inhibition ability of Clotrimazole on PFKP, IHC and immunofluorescence double staining were used to evaluate the expression of PFKP in glomeruli and podocyte respectively.

Clotrimazole purchased from MedChemExpress. Usage Cited in: Front Endocrinol. 2022 Jan 14;12:797025. [Abstract]

Western blotting analyses indicated that the innate and enhanced protein levels of PFKP were suppressed by Clotrimazole (100 mg/kg, ip, 2 weeks) administration in glomeruli from both db/m and db/db mice respectively.
J Virol

Identification of Clotrimazole Derivatives as Specific Inhibitors of Arenavirus Fusion. [Abstract]

2019 Mar 5;93(6):e01744-18. PMID: 30626681
BMC Cancer

TRPM2 channels mediate ROS-induced actin remodeling and cell migration of prostate cancer cells. [Abstract]

2025 May 28;25(1):956. PMID: 40437388
PLoS Negl Trop Dis

Identification of anti-flaviviral drugs with mosquitocidal and anti-Zika virus activity in Aedes aegypti. [Abstract]

2019 Aug 20;13(8):e0007681. PMID: 31430351

Solvent & Solubility

In Vitro:

DMSO : 33.33 mg/mL (96.65 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H₂O : < 0.1 mg/mL (insoluble)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

2.8999 mL

14.4995 mL

28.9990 mL

5 mM

0.5800 mL

2.8999 mL

5.7998 mL

10 mM

0.2900 mL

1.4499 mL

2.8999 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Molarity Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Concentration Concentration

Volume Volume

Molecular Weight Molecular Weight

Dilution Calculator

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

Concentration _(start) Concentration _(start)

Volume _(start) Volume _(start)

Concentration _(final) Concentration _(final)

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In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline

Solubility: ≥ 2.5 mg/mL (7.25 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)

Solubility: ≥ 2.5 mg/mL (7.25 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil

Solubility: ≥ 2.5 mg/mL (7.25 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

%
DMSO +

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
%
+
+
%
Tween-80 + +
%
Saline +

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Working solution concentration: 0.22 mg/mL

Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (281 KB)
SDS (596 KB)
- English - EN (596 KB)
- Français - FR (596 KB)
- Deutsch - DE (596 KB)
- Norwegian - NO (596 KB)
- Español - ES (596 KB)
- Swedish - SV (596 KB)
- Italian - IT (596 KB)
- Korean - KR (596 KB)
- Portuguese - PT (596 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Sawyer PR, Clotrimazole: a review of its antifungal activity and therapeutic efficacy. Drugs. 1975;9(6):424-47. [Content Brief]

[2]. Witzke A, Inhibition of the gastric H,K-ATPase by clotrimazole. Biochemistry. 2010 Jun 1;49(21):4524-32. [Content Brief]

[3]. Schaller K. In vitro antibacterial activity of different clotrimazole formulations. Chemotherapy. 1982;28 Suppl 1:32-6. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	2.8999 mL	14.4995 mL	28.9990 mL	72.4974 mL
	5 mM	0.5800 mL	2.8999 mL	5.7998 mL	14.4995 mL
	10 mM	0.2900 mL	1.4499 mL	2.8999 mL	7.2497 mL
	15 mM	0.1933 mL	0.9666 mL	1.9333 mL	4.8332 mL
	20 mM	0.1450 mL	0.7250 mL	1.4499 mL	3.6249 mL
	25 mM	0.1160 mL	0.5800 mL	1.1600 mL	2.8999 mL
	30 mM	0.0967 mL	0.4833 mL	0.9666 mL	2.4166 mL
	40 mM	0.0725 mL	0.3625 mL	0.7250 mL	1.8124 mL
	50 mM	0.0580 mL	0.2900 mL	0.5800 mL	1.4499 mL
	60 mM	0.0483 mL	0.2417 mL	0.4833 mL	1.2083 mL
	80 mM	0.0362 mL	0.1812 mL	0.3625 mL	0.9062 mL

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Clotrimazole

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Publications Citing Use of MedChemExpress (MCE) Clotrimazole

All Antibiotic Isoforms

Biological Activity

Chemical Information

Publications (9)

Solvent & Solubility

Purity & Documentation

References

Help & FAQs

Complete Stock Solution Preparation Table

Clotrimazole Related Classifications

HY-10882 Related Classifications