Flucytosine (Synonyms: 5-Fluorocytosine; NSC 103805; Ro 2-9915)

Name: Flucytosine
Brand: MedChemExpress
SKU: HY-B0139
Rating: 5.0 (3 reviews)

Cat. No.: HY-B0139 Purity: 99.99%: Data Sheet
COA

SDS
Handling Instructions
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Flucytosine (5-Fluorocytosine) is an antifungal compound with oral activity. Flucytosine is a widely used cytotoxic drug that, after further metabolism, produces fluorinated ribonucleotides and deoxyribonucleotides, inhibits DNA and protein synthesis, and has multiple effects such as inhibiting candida and candida neoplasm infection and producies cytotoxicity to cancer cells.

For research use only. We do not sell to patients.

CAS No. : 2022-85-7

Size	Stock	Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	In-stock	Estimated Time of Arrival: December 31
Solid
500 mg	In-stock	Estimated Time of Arrival: December 31
1 g	In-stock	Estimated Time of Arrival: December 31
5 g	In-stock	Estimated Time of Arrival: December 31
10 g	Get quote
50 g	Get quote

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Customer Review

Based on 3 publication(s) in Google Scholar

Other Forms of Flucytosine:

Flucytosine-¹³C,15N₂ In-stock
Flucytosine (Standard) In-stock

3 Publications Citing Use of MCE Flucytosine

Others

: Flucytosine purchased from MedChemExpress. Usage Cited in: Front Microbiol. 2024 Jul 30:15:1450557. [Abstract]; We evaluated the resistance of wild-type strain SC5314 and two strains carrying ChrR trisomy and tetrasomy mutations, respectively, to a range of antifungal drugs, including azoles (clotrimazole and miconazole), echinocandins (caspofungin), and pyrimidines (5-Fluorocytosine).

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Biological Activity
Purity & Documentation
References
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Description

Cellular Effect

Cell Line	Type	Value	Description	References
CCRF-CEM	IC₅₀	> 100 μM Compound: 5-Fluorocytosine	Cytotoxicity was determined in CEM cells, relative to RVT Cytotoxicity was determined in CEM cells, relative to RVT	[PMID: 15081000]
Vero	IC₅₀	> 100 μM Compound: 5-Fluorocytosine	Cytotoxicity was determined in Vero cells, relative to RVT Cytotoxicity was determined in Vero cells, relative to RVT	[PMID: 15081000]

In Vitro

Flucytosine transfected CD-gene hMSC (CD-hMSC) effectively converts 5-FC into 5-FU, showing anti-cancer therapeutic potential^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

Flucytosine (500 mg/kg/ day, intravenous injection for 7 days) can effectively reduce tumor volume and weight in CD-hMSC mice^[2].
Flucytosine (50, 75 mg/kg/ day, gavage, 3-30 days) combines with itraconazole (HY-17514) can effectively inhibit cryptococcus infection in hamsters^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	CD-hMSC mice^[2]
Dosage:	500 mg/kg
Administration:	i.v. for 7 days
Result:	Inhibited tumor volume and bodyweight.

Clinical Trial

Molecular Weight

129.09

Formula

C₄H₄FN₃O

CAS No.

2022-85-7

Appearance

Solid

Color

White to off-white

SMILES

O=C1NC=C(F)C(N)=N1

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	1 year
	-20°C	6 months

Solvent & Solubility

In Vitro:

DMSO : 16.67 mg/mL (129.13 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H₂O : 6.67 mg/mL (51.67 mM; Need ultrasonic)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	7.7465 mL	38.7327 mL	77.4653 mL
5 mM	1.5493 mL	7.7465 mL	15.4931 mL
10 mM	0.7747 mL	3.8733 mL	7.7465 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 1.67 mg/mL (12.94 mM); Clear solution

This protocol yields a clear solution of ≥ 1.67 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (16.7 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 1.67 mg/mL (12.94 mM); Clear solution

This protocol yields a clear solution of ≥ 1.67 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (16.7 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 1.67 mg/mL (12.94 mM); Suspended solution

This protocol yields a suspended solution of ≥ 1.67 mg/mL (saturation unknown). Suspended solution can be used for oral and intraperitoneal injection.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (16.7 mg/mL) to 900 μL Corn oil, and mix evenly.

For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: PBS
Solubility: 8.67 mg/mL (67.16 mM); Clear solution; Need ultrasonic

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 99.99%

Select Batch:

Data Sheet (277 KB) SDS (418 KB)

COA (252 KB) HNMR (117 KB) RP-HPLC (266 KB)

Handling Instructions (2659 KB)

References

[1]. Vermes A, et al. Flucytosine: a review of its pharmacology, clinical indications, pharmacokinetics, toxicity and drug interactions. J Antimicrob Chemother. 2000 Aug;46(2):171-9. [Content Brief]

[2]. You MH, et al. Cytosine deaminase-producing human mesenchymal stem cells mediate an antitumor effect in a mouse xenograft model. J Gastroenterol Hepatol. 2009 Aug;24(8):1393-400. [Content Brief]

[3]. Iovannitti C, et al. Itraconazole and flucytosine+itraconazole combination in the treatment of experimental cryptococcosis in hamsters. Mycoses. 1995 Nov-Dec;38(11-12):449-52. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

H₂O / DMSO	1 mM	7.7465 mL	38.7327 mL	77.4653 mL	193.6633 mL
	5 mM	1.5493 mL	7.7465 mL	15.4931 mL	38.7327 mL
	10 mM	0.7747 mL	3.8733 mL	7.7465 mL	19.3663 mL
	15 mM	0.5164 mL	2.5822 mL	5.1644 mL	12.9109 mL
	20 mM	0.3873 mL	1.9366 mL	3.8733 mL	9.6832 mL
	25 mM	0.3099 mL	1.5493 mL	3.0986 mL	7.7465 mL
	30 mM	0.2582 mL	1.2911 mL	2.5822 mL	6.4554 mL
	40 mM	0.1937 mL	0.9683 mL	1.9366 mL	4.8416 mL
	50 mM	0.1549 mL	0.7747 mL	1.5493 mL	3.8733 mL
DMSO	60 mM	0.1291 mL	0.6455 mL	1.2911 mL	3.2277 mL
	80 mM	0.0968 mL	0.4842 mL	0.9683 mL	2.4208 mL
	100 mM	0.0775 mL	0.3873 mL	0.7747 mL	1.9366 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Flucytosine Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Flucytosine2022-85-75-Fluorocytosine NSC 103805 Ro 2-9915NSC103805NSC 103805NSC-103805FungalAntibiotic5-FC5-FUMKN45CD-hMSCInhibitorinhibitorinhibit

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Flucytosine (Synonyms: 5-Fluorocytosine; NSC 103805; Ro 2-9915)

Customer Review

Other Forms of Flucytosine:

Flucytosine Related Antibodies

3 Publications Citing Use of MCE Flucytosine

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•Related Screening Libraries:

•Related Small Molecules:

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View All Antibiotic Isoform Specific Products:

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References

Customer Review

Flucytosine Related Antibodies

Molarity Calculator

Dilution Calculator

Complete Stock Solution Preparation Table

Flucytosine Related Classifications

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