Anidulafungin
Based on 6 publication(s) in Google Scholar
Anidulafungin is a new semisynthetic echinocandin with antifungal potency.
For research use only. We do not sell to patients.
- Purity: 99.69%
- CAS No.: 166663-25-8
- Formula: C58H73N7O17
- Molecular Weight:1140.24
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Storage:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 1 year , -20°C, 6 months
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Protocol
- Purity & Documentation
- References
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Help & FAQs
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Publications Citing Use of MedChemExpress (MCE) Anidulafungin
More- Adv Sci (Weinh). 2022 Dec;9(34):e2203088. [Abstract]
- J Clin Microbiol. 2017 Jun;55(6):1883-1893. [Abstract]
- J Fungi (Basel). 2024 May 31;10(6):397. [Abstract]
- mSphere. 2020 Oct 28;5(5):e00821-20. [Abstract]
- Mycoses. 2021 Mar;64(3):282-291. [Abstract]
- mSphere. 2018 Nov 14;3(6). pii: e00547-18. [Abstract]
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Bio/Physico-chemical Assay
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RT-PCR
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In Vivo Efficacy Study
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Biological Activity
Antifungal[1]
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Cell Line
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Type | Value | Description | References |
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| Vero | CC50 |
>50 μM
Compound: Anidulafungin
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Cell viability measured by CellTiter-Glo assay in Vero cells at MOI 0.05 after 72hr
Cell viability measured by CellTiter-Glo assay in Vero cells at MOI 0.05 after 72hr
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10.1101/2020.03.20.999730 |
| Vero | IC50 |
4.64 μM
Compound: Anidulafungin
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Antiviral activity against SARS-CoV-2 (viral titer) measured by plaque assay in Vero cells at MOI 0.0125 after 24 hr
Antiviral activity against SARS-CoV-2 (viral titer) measured by plaque assay in Vero cells at MOI 0.0125 after 24 hr
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10.1101/2020.03.20.999730 |
Anidulafungin (LY-303366) has MICs of ≤0.32 μg/mL for all Candida albicans (n=99), Candida glabrata (n=18), and Candida tropicalis (n=10) isolates tested. Anidulafungin is also active against Aspergillus species (minimum effective concentration at which 90% of the isolates are inhibited, 0.02 μg/mL) (n=20), is less active against Candida parapsilosis (MIC at which 90% of the isolates are inhibited [MIC90], 5.12 μg/mL) (n=10), and is inactive against C. neoformans (MIC90 >10.24 μg/mL) (n=15) and B. dermatitidis (MIC90, 16 μg/mL) (n=29).The MICs of Fluconazole for three strains of C. tropicalis, seven strains of C. glabrata, and two strains of Candida krusei are ≥16 μg/mL. The MICs of Anidulafungin for 11 of these 12 strains range from 0.08 to 0.32 mg/mL. The twelfth strain is a C. krusei strain (Fluconazole MIC, 32 μg/mL) for which the Anidulafungin MIC is 1.28 mg/mL. The MIC at which 90% of the isolates are inhibited (MIC90) for these 12 strains is 0.32 μg/mL. The Anidulafungin MIC90 for the remaining 18 C. glabrata isolates and C. tropicalis isolates for which the Fluconazole MICs are ≥ 8 μg/mL is also 0.32 mg/mL. Anidulafungin appeares equally active against Candida species for which the fluconazole MICs are ≥16 mg/mL and against those for which the fluconazole MICs are ≥ 8 μg/mL. Anidulafungin has significantly less activity against C. neoformans and B. dermatitidis than against C. albicans, C. glabrata, and C. tropicalis. Ketoconazole and amphotericin B are the most active antifungal agents tested for both C. neoformans and B. dermatitidis. Anidulafungin demonstrated potent in vitro activity against Aspergillus species with a MEC90 of 0.02 μg/mL. MICs of Anidulafungin for the control strain yeast isolates are 0.02 μg/mL for C. albicans ATCC 90028, 0.16 mg/mL for C. glabrata ATCC 90030, and >10.24 μg/mL for C. neoformans ATCC 90112[1]. Strains selected with CD101 that have a 2-fold or greater CD101 MIC increase also have at least a 2-fold MIC increase for Anidulafungin (ANF) and/or Caspofungin (CSF)[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
| NCT Number | Sponsor | Condition | Start Date |
Phase
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|---|---|---|---|---|
| NCT01329991 | Plexxikon| | 2011-05 | PHASE1 |
Chemical Information
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CAS No. 166663-25-8
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Appearance Solid
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Molecular Weight 1140.24
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Formula C58H73N7O17
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Color White to off-white
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SMILES
O[C@@H]1C[C@H](N2C1)C(N[C@@H]([C@H](O)[C@@H](O)C3=CC=C(O)C=C3)C(N[C@@H]([C@@H](C)O)C(N4[C@@H]([C@@H](O)[C@@H](C)C4)C(N[C@H](O)[C@H](O)C[C@H](NC(C5=CC=C(C6=CC=C(C7=CC=C(OCCCCC)C=C7)C=C6)C=C5)=O)C(N[C@@H]([C@H](O)C)C2=O)=O)=O)=O)=O)=O
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Synonyms
LY303366
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Powder -20°C 3 years 4°C 2 years In solvent -80°C 1 year -20°C 6 months
Publications (6)
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Journal Impact Factor
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Most Recent
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Adv Sci (Weinh)
SRSF5-Mediated Alternative Splicing of M Gene is Essential for Influenza A Virus Replication: A Host-Directed Target Against Influenza Virus. [Abstract]2022 Dec;9(34):e2203088. PMID: 36257906
Anidulafungin purchased from MedChemExpress. Usage Cited in: Adv Sci (Weinh). 2022 Dec;9(34):e2203088. [Abstract]
Anidulafungin (40 µM; 6 h) markedly inhibited PR8 virus replication in A549 cells.
Anidulafungin purchased from MedChemExpress. Usage Cited in: Adv Sci (Weinh). 2022 Dec;9(34):e2203088. [Abstract]
Anidulafungin (5-40 μM) significantly reduced the ratio of M2/M1 RNA during PR8 virus infection of A549 cells in a dose-dependent manner.
Anidulafungin purchased from MedChemExpress. Usage Cited in: Adv Sci (Weinh). 2022 Dec;9(34):e2203088. [Abstract]
Anidulafungin (20 mg/kg; p.o.; 12 d) prolonged survival of BALB/c mice infected with PR8 virus, with 80% of mice gaining body weight from day 6 of infection.
Anidulafungin purchased from MedChemExpress. Usage Cited in: Adv Sci (Weinh). 2022 Dec;9(34):e2203088. [Abstract]
Anidulafungin (20 mg/kg; p.o.; 6 d) significantly decreased infectious progeny from lung tissues of BALB/c mice infected with PR8 virus.
Anidulafungin purchased from MedChemExpress. Usage Cited in: Adv Sci (Weinh). 2022 Dec;9(34):e2203088. [Abstract]
Anidulafungin (20 mg/kg; p.o.; 6 d) alleviated severe lung pathology in BALB/c mice infected with PR8 virus.
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J Clin Microbiol
Antifungal Susceptibility Testing of Malassezia spp. with an Optimized Colorimetric Broth Microdilution Method. [Abstract]2017 Jun;55(6):1883-1893. PMID: 28381607 -
J Fungi (Basel)
In Vitro Activitiy of Rezafungin in Comparison with Anidulafungin and Caspofungin against Invasive Fungal Isolates (2017 to 2022) in China. [Abstract]2024 May 31;10(6):397. PMID: 38921383 -
mSphere
Triazole Evolution of Candida parapsilosis Results in Cross-Resistance to Other Antifungal Drugs, Influences Stress Responses, and Alters Virulence in an Antifungal Drug-Dependent Manner. [Abstract]2020 Oct 28;5(5):e00821-20. PMID: 33115837 -
Mycoses
Antifungal susceptibility testing of dermatophytes: Development and evaluation of an optimised broth microdilution method. [Abstract]2021 Mar;64(3):282-291. PMID: 33084127 -
mSphere
Echinocandin-Induced Microevolution of Candida parapsilosis Influences Virulence and Abiotic Stress Tolerance. [Abstract]2018 Nov 14;3(6). pii: e00547-18. PMID: 30429225
Solvent & Solubility
DMSO : ≥ 100 mg/mL (87.70 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
* "≥" means soluble, but saturation unknown.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Protocol
Stocks of CD101 (formerly SP 3025, biafungin, AF-025) are prepared fresh in 100% DMSO prior to use. The comparator antifungals Anidulafungin (ANF), Caspofungin (CSF), and Amphotericin B (AMB) are also prepared in 100% DMSO. MIC assays are performed via broth microdilution in accordance with CLSI guidelines, with the exception that test compounds are made up at a 50× final assay concentration and 100 μL assay mixture volumes are used (2 μL added to 98 μL of broth containing cells at 0.5×103 to 2.5×103 CFU/mL). All MIC assays are run at least three times, and a representative data set is shown. Quality control (QC) is assessed throughout the study via comparison of MIC values derived for WT C. krusei strain ATCC 6258 for AMB, CSF, and ANF with previously reported CLSI 24-h broth microdilution QC ranges[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Purity & Documentation
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Data Sheet (280 KB)
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SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Portuguese - PT (393 KB)
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Handling Instructions (2659 KB)
References
[1]. Zhanel GG, et al. In vitro activity of a new semisynthetic echinocandin, LY-303366, against systemic isolates of Candida species, Cryptococcus neoformans, Blastomyces dermatitidis, and Aspergillus species. Antimicrob Agents Chemother. 1997 Apr;41(4):863-5 [Content Brief]
[2]. Locke JB, et al. Characterization of In Vitro Resistance Development to the Novel Echinocandin CD101 in Candida Species. Antimicrob Agents Chemother. 2016 Sep 23;60(10):6100-7. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
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| DMSO | 1 mM | 0.8770 mL | 4.3850 mL | 8.7701 mL | 21.9252 mL |
| 5 mM | 0.1754 mL | 0.8770 mL | 1.7540 mL | 4.3850 mL | |
| 10 mM | 0.0877 mL | 0.4385 mL | 0.8770 mL | 2.1925 mL | |
| 15 mM | 0.0585 mL | 0.2923 mL | 0.5847 mL | 1.4617 mL | |
| 20 mM | 0.0439 mL | 0.2193 mL | 0.4385 mL | 1.0963 mL | |
| 25 mM | 0.0351 mL | 0.1754 mL | 0.3508 mL | 0.8770 mL | |
| 30 mM | 0.0292 mL | 0.1462 mL | 0.2923 mL | 0.7308 mL | |
| 40 mM | 0.0219 mL | 0.1096 mL | 0.2193 mL | 0.5481 mL | |
| 50 mM | 0.0175 mL | 0.0877 mL | 0.1754 mL | 0.4385 mL | |
| 60 mM | 0.0146 mL | 0.0731 mL | 0.1462 mL | 0.3654 mL | |
| 80 mM | 0.0110 mL | 0.0548 mL | 0.1096 mL | 0.2741 mL |
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