Rhodamine 6G perchlorate  (Synonyms: Basic Red 1 perchlorate)

Rhodamine dyes are membrane-permeable cationic fluorescent probes that specifically recognize mitochondrial membrane potentials, thereby attaching to mitochondria and producing bright fluorescence, and at certain concentrations, rhodamine dyes have low toxicity to cells, so they are commonly used to detect mitochondria in animal cells, plant cells, and microorganisms^[1][2][3][4].

Guide (The following is our recommended experimental protocol. This protocol serves merely as a reference guide; specific procedures should be adjusted according to your actual requirements.)
1. Preparation of Rhodamine 6G perchlorate Working Solution
1.1 Preparation of Stock Solution
Dilute 1 mg of Rhodamine 6G perchlorate with 525 μL of anhydrous DMSO to prepare a 5 mM stock solution.
1.2 Preparation of Working Solution
Dilute the stock solution with pre-warmed serum-free cell culture medium or PBS to prepare a Rhodamine 6G perchlorate working solution with a concentration of 1-20 μM.
Note: Please adjust the concentration of the Rhodamine 6G working solution according to your specific needs, and prepare it immediately before use.
2. Cell Staining (Suspension Cells)
2.1 Centrifuge to harvest the cells, then wash twice with PBS (5 minutes per wash). Adjust the cell density to 1 × 10⁶ cells/mL.
2.2 Add 1 mL of the Rhodamine 6G working solution and incubate at room temperature for 30–60 minutes.
2.3 Centrifuge at 400 × g for 3-4 minutes, and discard the supernatant.
2.4 Wash the cells twice with PBS (5 minutes per wash).
2.5 Resuspend the cells in 1 mL of serum-free medium or PBS, then observe using a fluorescence microscope or flow cytometer.
3. Cell Staining (Adherent Cells)
3.1 Culture the adherent cells on sterile coverslips.
3.2 Remove the coverslips from the culture medium and aspirate any excess medium.
3.3 Add 100 μL of the dye working solution, gently agitate to ensure the solution completely covers the cells, and incubate for 5-30 minutes.
3.4 Aspirate the working dye solution, wash with culture medium 2-3 times (5 minutes each), and observe using a fluorescence microscope or flow cytometer.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

543.01

C₂₈H₃₁ClN₂O₇

13161-28-9

CCNC1=CC2=C(C=C1C)C(=C3C=C(C)/C(=N/CC)/C=C3O2)C4=CC=CC=C4C(=O)OCC.Cl(=O)(=O)(=O)O

Room temperature in continental US; may vary elsewhere.

Please store the product under the recommended conditions in the Certificate of Analysis.

• [1]. Zehentbauer FM, et al. Fluorescence spectroscopy of Rhodamine 6G: concentration and solvent effects. Spectrochim Acta A Mol Biomol Spectrosc. 2014;121:147-51.  [Content Brief]

  [2]. Kutushov M, et al. Low concentrations of Rhodamine 6G selectively destroy tumor cells and improve survival of melanoma transplanted mice. Neoplasma. 2013;60(3):262-73.  [Content Brief]

  [3]. Kutushov M, et al. Low concentrations of Rhodamine 6G selectively destroy tumor cells and improve survival of melanoma transplanted mice. Neoplasma. 2013;60(3):262-73.  [Content Brief]

  [4]. Jain RK, et al. Measuring leukocyte-endothelial interactions in mice. Cold Spring Harb Protoc. 2013 Jun 1;2013(6):561-3.  [Content Brief]