8-Phenyl-BODIPY 505/515

Name: 8-Phenyl-BODIPY 505/515
Brand: MedChemExpress
SKU: HY-W089353
Rating: 4.5 (1 reviews)

Cat. No.: HY-W089353 Purity: 98.47%: Data Sheet
COA

SDS
Handling Instructions
FAQs
Technical Support

8-Phenyl-BODIPY 505/515 is a molecular rotor based on the BODIPY scaffold, specifically used as a microviscosity probe. The working principle of 8-Phenyl-BODIPY 505/515 is based on the restriction of molecular motion by viscosity: in a high-viscosity environment, the rotational freedom of its chemical bonds is inhibited, which leads to a significant extension of fluorescence lifetime. This property makes 8-Phenyl-BODIPY 505/515 an effective tool for monitoring changes in the viscosity of intracellular microenvironments via fluorescence lifetime imaging (FLIM) technology.

For research use only. We do not sell to patients.

8-Phenyl-BODIPY 505/515 Chemical Structure

CAS No. : 194235-40-0

Size	Stock	Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	In-stock	Estimated Time of Arrival: December 31
Solid
10 mg	In-stock	Estimated Time of Arrival: December 31
25 mg	In-stock	Estimated Time of Arrival: December 31
50 mg	In-stock	Estimated Time of Arrival: December 31
100 mg	In-stock	Estimated Time of Arrival: December 31
200 mg	Get quote
500 mg	Get quote

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Customer Review

Based on 1 publication(s) in Google Scholar

1 Publications Citing Use of MCE 8-Phenyl-BODIPY 505/515

•iScience. 2025 Jun 19;28(7):112954. [Abstract]

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Biological Activity
Purity & Documentation
References
Customer Review

Description

In Vitro

8-Phenyl-BODIPY 505/515 detects a local microviscosity maximum 10-20 μm above untreated F1-4_ErbB2 cells, and this region of increased microviscosity shifts to the membrane surface in enzyme-treated F1-4_ErbB2 cells, confirming its association with extracellular matrix components^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

324.18

Formula

C₁₉H₁₉BF₂N₂

CAS No.

194235-40-0

Appearance

Solid

Color

Orange to red

Emission (Em)

515

Excitation (Ex)

502

SMILES

CC1=C([N-](C(C)=C1)[B+3]([F-])2[F-])C(C3=CC=CC=C3)=C(C(C)=C4)[N]2=C4C

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, sealed storage, away from moisture and light

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

Solvent & Solubility

In Vitro:

DMSO : 40 mg/mL (123.39 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	3.0847 mL	15.4235 mL	30.8471 mL
5 mM	0.6169 mL	3.0847 mL	6.1694 mL
10 mM	0.3085 mL	1.5424 mL	3.0847 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

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μL

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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

Purity & Documentation

Purity: 99.78%

Select Batch:

Data Sheet (280 KB) SDS (393 KB)

COA (260 KB) HNMR (118 KB) RP-HPLC (215 KB) LCMS (100 KB)

Handling Instructions (2659 KB)

References

[1]. Szabó Á, et al. Local ligand concentration gradients induced by the plasma membrane. iScience. 2025;28(7):112954. Published 2025 Jun 19. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	3.0847 mL	15.4235 mL	30.8471 mL	77.1177 mL
	5 mM	0.6169 mL	3.0847 mL	6.1694 mL	15.4235 mL
	10 mM	0.3085 mL	1.5424 mL	3.0847 mL	7.7118 mL
	15 mM	0.2056 mL	1.0282 mL	2.0565 mL	5.1412 mL
	20 mM	0.1542 mL	0.7712 mL	1.5424 mL	3.8559 mL
	25 mM	0.1234 mL	0.6169 mL	1.2339 mL	3.0847 mL
	30 mM	0.1028 mL	0.5141 mL	1.0282 mL	2.5706 mL
	40 mM	0.0771 mL	0.3856 mL	0.7712 mL	1.9279 mL
	50 mM	0.0617 mL	0.3085 mL	0.6169 mL	1.5424 mL
	60 mM	0.0514 mL	0.2571 mL	0.5141 mL	1.2853 mL
	80 mM	0.0386 mL	0.1928 mL	0.3856 mL	0.9640 mL
	100 mM	0.0308 mL	0.1542 mL	0.3085 mL	0.7712 mL