IR-820  (Synonyms: New Indocyanine Green)

IR-820 (New Indocyanine Green) is an infrared blood pool contrast agent. IR-820 also is normally used as a laser and near-infrared dye to detect and quantify diseased tissue in live animals^[1].

Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).
In vivo^[1]:
1. The powdered IR-820 was freshly mixed with phosphate buffer saline (PBS) to a final concentration of 0.2 mM and 100 μl of this solution was used for injecting into experimental animals.
2. Used 2 groups of four Hairless SKHi/sKHl mice. The first group was injected with IR-820 by intravenous tail vein injection while the second was injected intraperitoneally.
3. IR-820 has maximal excitation and emission wavelengths of 710 nm and 820 nm, respectively. Both groups were serially imaged for 8 days.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

849.47

C₄₆H₅₀ClN₂NaO₆S₂

172616-80-7

Solid

Brown to dark brown

845

820

CC1(C(C2=CC=CC=C2C=C3)=C3[N+](CCCC[S](=O)([O-])=O)=C1/C=C/C(CCC/C4=C\C=C(C5(C)C)/N(C6=C5C7=CC=CC=C7C=C6)CCCC[S](=O)(O[Na])=O)=C4Cl)C

Room temperature in continental US; may vary elsewhere.

4°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

• [1]. Suresh I Prajapati, et al. Near-infrared imaging of injured tissue in living subjects using IR-820. Mol Imaging. 2009 Jan-Feb;8(1):45-54.  [Content Brief]