Acid Red 94 sodium
Based on 1 publication(s) in Google Scholar
Acid Red 94 sodium, a synthetic fluorescein derivative, is a deep red dye primarily composed of 4,5,6,7-tetrachloro-2,4,5,7-tetraiodo fluorescein. It is widely used as an ophthalmic diagnostic agent to detect dry or damaged cells on the ocular surface. Acid Red 94 sodium exhibits antitumor activity and can inhibit Friend Leukemia Virus (FLV) infection through photodynamic action. Additionally, Acid Red 94 sodium can inhibit Aβ aggregation through light irradiation. Acid Red 94 sodium holds potential for use in cancer, viral infections, and neurodegenerative disease research.
For research use only. We do not sell to patients.
- Purity: 99.49%
- CAS No.: 632-69-9
- Formula: C20H2Cl4I4Na2O5
- Molecular Weight:1017.64
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Storage:
RT, sealed storage, away from moisture and light.
In solvent -80°C, 1 year , -20°C, 6 months
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Publications Citing Use of MedChemExpress (MCE) Acid Red 94 sodium
More
Biological Activity
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| B16-F10 | IC50 |
>5 μM
Compound: 1; RBNa
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Photodynamic anticancer activity in mouse B16F10 cells preincubated for 2 hrs followed by light exposure at 27 J/cm2 for 30 mins and subsequent incubation for 24 hrs by MTT assay
Photodynamic anticancer activity in mouse B16F10 cells preincubated for 2 hrs followed by light exposure at 27 J/cm2 for 30 mins and subsequent incubation for 24 hrs by MTT assay
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[PMID: 29324346] |
| HepG2 | IC50 |
>5 μM
Compound: 1; RBNa
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Photodynamic anticancer activity in human HepG2 cells preincubated for 2 hrs followed by light exposure at 27 J/cm2 for 30 mins and subsequent incubation for 24 hrs by MTT assay
Photodynamic anticancer activity in human HepG2 cells preincubated for 2 hrs followed by light exposure at 27 J/cm2 for 30 mins and subsequent incubation for 24 hrs by MTT assay
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[PMID: 29324346] |
| MCF7 | IC50 |
>5 μM
Compound: 1; RBNa
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Photodynamic anticancer activity in human MCF7 cells preincubated for 2 hrs followed by light exposure at 27 J/cm2 for 30 mins and subsequent incubation for 24 hrs by MTT assay
Photodynamic anticancer activity in human MCF7 cells preincubated for 2 hrs followed by light exposure at 27 J/cm2 for 30 mins and subsequent incubation for 24 hrs by MTT assay
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[PMID: 29324346] |
Acid Red 94 (300 µM, 48 h) exerts toxic effects on MCF-7 cells by inducing apoptosis[3]. Acid Red 94 (5 µM, under light irradiation, 24 h) inhibits Aβ aggregation, likely by binding to Ab42 and preventing its conformational transition[4].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:MCF-7 (Breast cancer)
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Concentration:100 µM, 200 µM, 300 µM
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Incubation Time:24 h, 48 h, 72 h
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Result:Decreased MCF-7 cell viability in a concentration- and time-dependent manner.
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Cell Line:MCF-7 (Breast cancer)
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Concentration:300 µM
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Incubation Time:48 h
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Result:Significantly increased Bax protein expression.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:BALB/c mice bearing MT-901 breast cancer model[5]
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Dosage:50 µL (10% Rose Bengal solution, PV-10, prepared by dissolving 10% Rose Bengal disodium salt in 0.9% NaCl (saline solution))
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Administration:Intralesional injection (IL) injected into the tumor lesion, once a week, total duration 14 days
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Result:Significantly reduced the volume of both the injected tumors and the lung metastases.
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Animal Model:Friend Leukemia Virus (FLV) infection in BALB/c mice model[6]
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Dosage:7 µg, 22 µg, 67 µg, 200 µg
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Administration:Intraperitoneal injection (i.p.), mixed with FLV inoculum, illuminated for 1 hour (photodynamic action)
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Result:Significantly inhibited FLV infection upon illumination at low doses.
Chemical Information
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CAS No. 632-69-9
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Appearance Solid
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Molecular Weight 1017.64
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Formula C20H2Cl4I4Na2O5
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Color Brown to reddish brown
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SMILES
O=C1OC2(C3=C(OC4=C2C=C(I)C(O[Na])=C4I)C(I)=C(O[Na])C(I)=C3)C5=C1C(Cl)=C(Cl)C(Cl)=C5Cl
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
RT, sealed storage, away from moisture and light
In solvent -80°C 1 year -20°C 6 months
Publications (1)
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Journal Impact Factor
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Most Recent
Solvent & Solubility
H2O : 31.25 mg/mL (30.71 mM; Need ultrasonic)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Purity & Documentation
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Data Sheet (287 KB)
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SDS (396 KB)
- English - EN (396 KB)
- Français - FR (396 KB)
- Deutsch - DE (396 KB)
- Norwegian - NO (396 KB)
- Español - ES (396 KB)
- Swedish - SV (396 KB)
- Italian - IT (396 KB)
- Korean - KR (396 KB)
- Portuguese - PT (396 KB)
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Handling Instructions (2659 KB)
References
[1]. Doughty MJ, et, al. Fluorescence characteristics of sodium fluorescein-rose bengal ophthalmic solution mixtures. Cont Lens Anterior Eye. 2014 Oct; 37(5): 358-62. [Content Brief]
[2]. Roat MI, et, al. The antiviral effects of rose bengal and fluorescein. Arch Ophthalmol. 1987 Oct; 105(10): 1415-7. [Content Brief]
[3]. Mousavi SH, et al. Direct toxicity of Rose Bengal in MCF-7 cell line: role of apoptosis. Food Chem Toxicol. 2009 Apr;47(4):855-9. [Content Brief]
[4]. Lee JS, et al. Photo-induced inhibition of Alzheimer's β-amyloid aggregation in vitro by rose bengal. Biomaterials. 2015 Jan;38:43-9. [Content Brief]
[5]. Toomey P, et al. Intralesional injection of rose bengal induces a systemic tumor-specific immune response in murine models of melanoma and breast cancer. PLoS One. 2013 Jul 17;8(7):e68561. [Content Brief]
[6]. Stevenson NR, Lenard J. Antiretroviral activities of hypericin and rose bengal: photodynamic effects on Friend leukemia virus infection of mice. Antiviral Res. 1993 Jun;21(2):119-27. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| H2O | 1 mM | 0.9827 mL | 4.9133 mL | 9.8267 mL | 24.5666 mL |
| 5 mM | 0.1965 mL | 0.9827 mL | 1.9653 mL | 4.9133 mL | |
| 10 mM | 0.0983 mL | 0.4913 mL | 0.9827 mL | 2.4567 mL | |
| 15 mM | 0.0655 mL | 0.3276 mL | 0.6551 mL | 1.6378 mL | |
| 20 mM | 0.0491 mL | 0.2457 mL | 0.4913 mL | 1.2283 mL | |
| 25 mM | 0.0393 mL | 0.1965 mL | 0.3931 mL | 0.9827 mL | |
| 30 mM | 0.0328 mL | 0.1638 mL | 0.3276 mL | 0.8189 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Acid Red 94 sodium Related Classifications
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