SJB3-019A
Based on 13 publication(s) in Google Scholar
SJB3-019A is a potent and novel USP1 inhibitor, 5 times more potent than SJB2-043 in promoting ID1 degradation and cytoxicity in K562 cells with IC50 of 0.0781 μM.
For research use only. We do not sell to patients.
- Purity: 99.88%
- CAS No.: 2070015-29-9
- Formula: C16H8N2O3
- Molecular Weight:276.25
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Storage:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 2 years , -20°C, 1 year
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
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Cell Cycle/DNA Damage Compound Library
HY-L004
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Anti-Cancer Compound Library
HY-L025
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Anti-Aging Compound Library
HY-L034
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Ubiquitination Compound Library
HY-L050
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Metabolic Enzyme Compound Library
HY-L146
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Protease Inhibitor Library
HY-L147
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Bioactive Compound Library Max
HY-L181
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Bioactive Compound Library
HY-L001
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High-Throughput Bioactive Compound Library
HY-L205
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Posttranslational Modification Library
HY-L226
Publications Citing Use of MedChemExpress (MCE) SJB3-019A
More- Blood (2016) 128 (22): 3290.
- Blood. 2015, 126 (23): 1813.
- Mol Cell. 2020 Nov 19;80(4):633-647.e7. [Abstract]
- Sci Adv. 2024 Nov 15;10(46):eadp6567. [Abstract]
- Sci Adv. 2019 May 8;5(5):eaav3235. [Abstract]
- Blood Cancer J. 2024 Nov 26;14(1):207. [Abstract]
- Cell Chem Biol. 2021 Jun 17;28(6):855-865.e9. [Abstract]
- J Med Chem. 2022 Oct 27;65(20):13645-13659. [Abstract]
- Eur J Pharm Biopharm. 2023 Dec:193:187-197. [Abstract]
- J Biol Chem. 2025 Jan 13:108190. [Abstract]
- Biomedicines. 2020 Jun 4;8(6):152. [Abstract]
- Int J Med Sci. 2021 Jan 1;18(1):245-255. [Abstract]
- University of Washington. 2025.
Biological Activity
IC50: 78.1 nM (ID1)
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| A549 | IC50 |
662 nM
Compound: SJB3-019A
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Antiproliferative activity against human A549 cells harboring KRAS mutant incubated for 72 hrs by MTT assay
Antiproliferative activity against human A549 cells harboring KRAS mutant incubated for 72 hrs by MTT assay
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[PMID: 36221183] |
| EBC-1 | IC50 |
122 nM
Compound: SJB3-019A
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Antiproliferative activity against human EBC-1 cells harboring wild type KRAS incubated for 72 hrs by MTT assay
Antiproliferative activity against human EBC-1 cells harboring wild type KRAS incubated for 72 hrs by MTT assay
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[PMID: 36221183] |
| NCI-H1703 | IC50 |
93 nM
Compound: SJB3-019A
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Antiproliferative activity against human NCI-H1703 cells harboring wild type KRAS incubated for 72 hrs by MTT assay
Antiproliferative activity against human NCI-H1703 cells harboring wild type KRAS incubated for 72 hrs by MTT assay
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[PMID: 36221183] |
| NCI-H1975 | IC50 |
98 nM
Compound: SJB3-019A
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Antiproliferative activity against human NCI-H1975 cells harboring wild type KRAS incubated for 72 hrs by MTT assay
Antiproliferative activity against human NCI-H1975 cells harboring wild type KRAS incubated for 72 hrs by MTT assay
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[PMID: 36221183] |
| NCI-H23 | IC50 |
281 nM
Compound: SJB3-019A
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Antiproliferative activity against human NCI-H23 cells harboring KRAS mutant incubated for 72 hrs by MTT assay
Antiproliferative activity against human NCI-H23 cells harboring KRAS mutant incubated for 72 hrs by MTT assay
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[PMID: 36221183] |
SJB3-019A (IC50=0.0781 μM) is 5 times more potent than SJB2-043 in promoting ID1 degradation and cytoxicity in K562 cells. SJB3-019A increases the levels of Ub-FANCD2 and Ub-PCNA, and decreases the HR activity[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 2070015-29-9
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Appearance Solid
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Molecular Weight 276.25
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Formula C16H8N2O3
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Color Light yellow to yellow
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SMILES
O=C1C2=C(OC(C3=CC=CN=C3)=N2)C(C4=CC=CC=C41)=O
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Powder -20°C 3 years 4°C 2 years In solvent -80°C 2 years -20°C 1 year
Publications (13)
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Journal Impact Factor
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Most Recent
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Mol Cell
2020 Nov 19;80(4):633-647.e7. PMID: 33217317 -
Sci Adv
2024 Nov 15;10(46):eadp6567. PMID: 39536107 -
Sci Adv
USP1 links platinum resistance to cancer cell dissemination by regulating Snail stability. [Abstract]2019 May 8;5(5):eaav3235. PMID: 31086816 -
Blood Cancer J
2024 Nov 26;14(1):207. PMID: 39592591 -
Cell Chem Biol
Development of potent and selective inhibitors targeting the papain-like protease of SARS-CoV-2. [Abstract]2021 Jun 17;28(6):855-865.e9. PMID: 33979649 -
J Med Chem
Discovery of Potent OTUB1/USP8 Dual Inhibitors Targeting Proteostasis in Non-Small-Cell Lung Cancer. [Abstract]2022 Oct 27;65(20):13645-13659. PMID: 36221183 -
Eur J Pharm Biopharm
Inhibition of USP1 ameliorates hypertensive nephropathy through regulating oxidative stress and ferroptosis: a precise treatment via SJB3-019A nanodelivery. [Abstract]2023 Dec:193:187-197. PMID: 37949326 -
J Biol Chem
2025 Jan 13:108190. PMID: 39814232 -
Biomedicines
A Human DUB Protein Array for Clarification of Linkage Specificity of Polyubiquitin Chain and Application to Evaluation of Its Inhibitors. [Abstract]2020 Jun 4;8(6):152. PMID: 32512835 -
Int J Med Sci
Inhibition of USP1 induces apoptosis via ID1/AKT pathway in B-cell acute lymphoblastic leukemia cells. [Abstract]2021 Jan 1;18(1):245-255. PMID: 33390793 -
Solvent & Solubility
DMSO : 10 mg/mL (36.20 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
For the following dissolution methods, please prepare the working solution directly:
It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 15% Cremophor EL 85% Saline
Solubility: 10 mg/mL (36.20 mM); Suspended solution; Need ultrasonic
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
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Data Sheet (274 KB)
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SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Portuguese - PT (393 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 3.6199 mL | 18.0995 mL | 36.1991 mL | 90.4977 mL |
| 5 mM | 0.7240 mL | 3.6199 mL | 7.2398 mL | 18.0995 mL | |
| 10 mM | 0.3620 mL | 1.8100 mL | 3.6199 mL | 9.0498 mL | |
| 15 mM | 0.2413 mL | 1.2066 mL | 2.4133 mL | 6.0332 mL | |
| 20 mM | 0.1810 mL | 0.9050 mL | 1.8100 mL | 4.5249 mL | |
| 25 mM | 0.1448 mL | 0.7240 mL | 1.4480 mL | 3.6199 mL | |
| 30 mM | 0.1207 mL | 0.6033 mL | 1.2066 mL | 3.0166 mL |
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