Sinensetin (Synonyms: Pedalitin permethyl ether)

Name: Sinensetin
Brand: MedChemExpress
SKU: HY-N0297
Rating: 5.0 (9 reviews)

Cat. No.: HY-N0297 Purity: 99.62%: Data Sheet
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Sinensetin is a methylated flavonoid found in fruits that has strong anti-vascular and anti-inflammatory properties.

For research use only. We do not sell to patients.

CAS No. : 2306-27-6

Size	Stock	Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	In-stock	Estimated Time of Arrival: December 31
Solid
1 mg	In-stock	Estimated Time of Arrival: December 31
5 mg	In-stock	Estimated Time of Arrival: December 31
10 mg	In-stock	Estimated Time of Arrival: December 31
25 mg	In-stock	Estimated Time of Arrival: December 31
50 mg	In-stock	Estimated Time of Arrival: December 31
100 mg	In-stock	Estimated Time of Arrival: December 31
200 mg	Get quote
500 mg	Get quote

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Customer Review

Based on 9 publication(s) in Google Scholar

Other Forms of Sinensetin:

Sinensetin (Standard) In-stock

9 Publications Citing Use of MCE Sinensetin

Cell Proliferation/Viability Assay

Flow Cytometry

Cell Imaging/Staining

Apoptosis Analysis

: Sinensetin purchased from MedChemExpress. Usage Cited in: Nutrients. 2020 Aug 15;12(8):2462. [Abstract]; HepG2 and Thle2 cells were treated with indicated concentrations of Sinensetin (SIN) (0, 25, 50, 75, and 100 μM) for 24 h or 48 h, and cell viability was determined by the MTT assay.

: Sinensetin purchased from MedChemExpress. Usage Cited in: Nutrients. 2020 Aug 15;12(8):2462. [Abstract]; HepG2 cells were treated with Sinensetin (SIN) (0, 50, 100 μM) for 48 h. Immunoblotting analysis of apoptosis markers, cleaved-PARP, cleaved-caspase-3, and Bcl-xL, was performed.

: Sinensetin purchased from MedChemExpress. Usage Cited in: Nutrients. 2020 Aug 15;12(8):2462. [Abstract]; HepG2 cells were treated with Sinensetin (SIN) (0, 50, 100 μM) for 48 h, then fixed and stained with propidium iodide (PI). Cell cycle was analyzed with flow cytometry.

: Sinensetin purchased from MedChemExpress. Usage Cited in: Nutrients. 2020 Aug 15;12(8):2462. [Abstract]; The images above shows HepG2 cells treated with Sinensetin (SIN) (0, 50, 100 μM) for 48 h. The cell morphology was examined by phase-contrast microscopy.

: Sinensetin purchased from MedChemExpress. Usage Cited in: Nutrients. 2020 Aug 15;12(8):2462. [Abstract]; Apoptosis was identified by annexin V-propidium iodide (PI) staining and analyzed by flow cytometry treated with Sinensetin (SIN) (0, 50, 100 μM).

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Biological Activity
Purity & Documentation
References
Customer Review

Description

Sinensetin is a methylated flavonoid found in fruits that has strong anti-vascular and anti-inflammatory properties.

Cellular Effect

Cell Line	Type	Value	Description	References
3T3-L1	IC₅₀	59 μM Compound: 5, sinensetin	Anticorpulence activity against mouse 3T3L1 cells assessed as inhibition of lipid droplet accumulation Anticorpulence activity against mouse 3T3L1 cells assessed as inhibition of lipid droplet accumulation	[PMID: 19054677]
A2780 ADR	IC₅₀	34 μM Compound: 19	Inhibition of P-gp expressed in A2780adr cells by calcein AM accumulation assay Inhibition of P-gp expressed in A2780adr cells by calcein AM accumulation assay	[PMID: 21354800]
HL-60	IC₅₀	> 100 μM Compound: V	Antiproliferative activity against HL60 after 24 hrs Antiproliferative activity against HL60 after 24 hrs	[PMID: 17391969]
HT-29	IC₅₀	67 μM Compound: 5, sinensetin	Anticancer activity against human HT-29 cells after 72 hrs by MTT assay Anticancer activity against human HT-29 cells after 72 hrs by MTT assay	[PMID: 19054677]
MCF-10A	IC₅₀	65 μM Compound: Sinensetin	Cytotoxicity against human MCF-10A cells assessed as inhibition of cell growth by MTT assay Cytotoxicity against human MCF-10A cells assessed as inhibition of cell growth by MTT assay	[PMID: 33257172]
MCF7	IC₅₀	8.3 μM Compound: 19	Inhibition of BCRP expressed in MCF-7 MX cells using Hoechst 33342 staining Inhibition of BCRP expressed in MCF-7 MX cells using Hoechst 33342 staining	[PMID: 21354800]
MDA-MB-231	EC₅₀	268.7 μM Compound: 22	Cytotoxicity against human MDA-MB-231 cells Cytotoxicity against human MDA-MB-231 cells	[PMID: 28112509]
MDA-MB-468	IC₅₀	0.2 μM Compound: Sinensetin	Antiproliferative activity against human MDA-MB-468 cells assessed as inhibition of cell growth by MTT assay Antiproliferative activity against human MDA-MB-468 cells assessed as inhibition of cell growth by MTT assay	[PMID: 33257172]
MDCK	IC₅₀	29 μM Compound: 19	Inhibition of MDR1 expressed in MDCK cells using rhodamine 123 staining by flow cytometry Inhibition of MDR1 expressed in MDCK cells using rhodamine 123 staining by flow cytometry	[PMID: 21354800]
MDCK	IC₅₀	9 μM Compound: 19	Inhibition of BCRP expressed in MDCK cells using Hoechst 33342 staining Inhibition of BCRP expressed in MDCK cells using Hoechst 33342 staining	[PMID: 21354800]
Monocyte	IC₅₀	1 x 10^-5 M Compound: sinensetin	Inhibition of procoagulant activity in monocyte from human blood assessed as counteraction of IL1-induced tissue factor expression after 18 hrs Inhibition of procoagulant activity in monocyte from human blood assessed as counteraction of IL1-induced tissue factor expression after 18 hrs	[PMID: 8882428]
Monocyte	IC₅₀	8 μM Compound: Sinensetin	Inhibition of TNFalpha expression in LPS-stimulated human monocytes treated 30 mins before LPS challenge measured after 14 hrs by ELISA Inhibition of TNFalpha expression in LPS-stimulated human monocytes treated 30 mins before LPS challenge measured after 14 hrs by ELISA	[PMID: 10096854]

In Vitro

Sinensetin (40 μM, 2 d) enhances adipogenesis of 3T3-L1 preadipocytes by up-regulating the adipogenic transcription factors in the absence of IBMX^[1].
Sinensetin (12-200 μM, 24-48 h) shows significant cytotoxic effects on Jurkat and CCRF-CEM cells in a dose-dependent and time-dependent manner^[4].
Sinensetin (100 μM, 48h) induces the sub-G1 phase and apoptosis in Jurkat cells^[4].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Western Blot Analysis^[1]

Cell Line:	3T3-L1
Concentration:	2, 10, 40 μM
Incubation Time:	24 d
Result:	Increased cellular lipid accumulation and triglyceride content in a dose-dependent manner. Increased the expression of PPARγ1, PPARγ2, C/EBPα, and aP2.

Cell Proliferation Assay^[4]

Cell Line:	CCRF-CEM cell, Jurkat
Concentration:	6.25–100 μM
Incubation Time:	24 or 48 h
Result:	Inhibited cell viability with different concentrations of sinensetin for 24 h and 48 h.

Apoptosis Analysis^[4]

Cell Line:	Jurkat cell
Concentration:	50 μM, 100 μM
Incubation Time:	24 h and 48 h
Result:	Induced a sub-G1 population and apoptotic.

In Vivo

Sinensetin (50 mg/kg, single dose, i.p.) has anti-inflammatory effects on carrageenan (HY-125474) induced paw inflammation in the mouse^[5].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	Carrageenan-induced paw edema in male C57BL/6 mice^[5]
Dosage:	50 mg/kg, single dose
Administration:	Intraperitoneal injection (i.p.)
Result:	Sloewd the volume increased of the carrageenan-treated paw at 6 h.

Molecular Weight

372.37

Formula

C₂₀H₂₀O₇

CAS No.

2306-27-6

Appearance

Solid

Color

White to light yellow

SMILES

O=C1C=C(C2=CC=C(OC)C(OC)=C2)OC3=CC(OC)=C(OC)C(OC)=C13

Structure Classification

Initial Source

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	2 years
	-20°C	1 year

Solvent & Solubility

In Vitro:

DMSO : 18.18 mg/mL (48.82 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.6855 mL	13.4275 mL	26.8550 mL
5 mM	0.5371 mL	2.6855 mL	5.3710 mL
10 mM	0.2686 mL	1.3428 mL	2.6855 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 1.25 mg/mL (3.36 mM); Clear solution

This protocol yields a clear solution of ≥ 1.25 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (12.5 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 1.25 mg/mL (3.36 mM); Clear solution

This protocol yields a clear solution of ≥ 1.25 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (12.5 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 99.62%

Select Batch:

Data Sheet (280 KB) SDS (393 KB)

COA (249 KB) HNMR (248 KB) LCMS (95 KB)

Handling Instructions (2659 KB)

References

[1]. Kang SI et al. Sinensetin enhances adipogenesis and lipolysis by increasing cyclic adenosine monophosphate levels in 3T3-L1 adipocytes. Biol Pharm Bull. 2015;38(4):552-8. [Content Brief]

[2]. Shin HS et al. Sinensetin attenuates LPS-induced inflammation by regulating the protein level of IκB-α. Biosci Biotechnol Biochem. 2012;76(4):847-9. [Content Brief]

[3]. Lam IK et al. In vitro and in vivo structure and activity relationship analysis of polymethoxylated flavonoids: identifying sinensetin as a novel antiangiogenesis agent. Mol Nutr Food Res. 2012 Jun;56(6):945-56. [Content Brief]

[4]. Kok-Tong Tan, et al. Sinensetin induces apoptosis and autophagy in the treatment of human T-cell lymphoma. Anticancer Drugs. 2019, 30, 5. [Content Brief]

[5]. Mirka Laavola, et al. Flavonoids eupatorin and sinensetin present in Orthosiphon stamineus leaves inhibit inflammatory gene expression and STAT1 activation. Planta Med. 2012, 78, 8. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	2.6855 mL	13.4275 mL	26.8550 mL	67.1375 mL
	5 mM	0.5371 mL	2.6855 mL	5.3710 mL	13.4275 mL
	10 mM	0.2686 mL	1.3428 mL	2.6855 mL	6.7138 mL
	15 mM	0.1790 mL	0.8952 mL	1.7903 mL	4.4758 mL
	20 mM	0.1343 mL	0.6714 mL	1.3428 mL	3.3569 mL
	25 mM	0.1074 mL	0.5371 mL	1.0742 mL	2.6855 mL
	30 mM	0.0895 mL	0.4476 mL	0.8952 mL	2.2379 mL
	40 mM	0.0671 mL	0.3357 mL	0.6714 mL	1.6784 mL

Sinensetin Related Classifications

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Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Sinensetin2306-27-6Pedalitin permethyl etherPGE synthaseTNF ReceptorProstaglandin E synthaseTumor Necrosis Factor ReceptorTNFRTNF Receptor InhibitorInhibitorinhibitorinhibit

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Sinensetin (Synonyms: Pedalitin permethyl ether)

Customer Review

Other Forms of Sinensetin:

Sinensetin Related Antibodies

9 Publications Citing Use of MCE Sinensetin

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•Related Screening Libraries:

•Related Small Molecules:

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View All TNF Receptor Isoform Specific Products:

Biological Activity

Purity & Documentation

References

Customer Review

Sinensetin Related Antibodies

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Complete Stock Solution Preparation Table

Sinensetin Related Classifications

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