1. Immunology/Inflammation
  2. COX
  3. (S)-(+)-Ibuprofen

(S)-(+)-Ibuprofen  (Synonyms: (S)-Ibuprofen)

Cat. No.: HY-78131A Purity: 99.79%
COA Handling Instructions

(S)-(+)-Ibuprofen ((S)-Ibuprofen), a S(+)-enantiomer of Ibuprofen, is a potent COX-1 and COX-2 inhibitor with IC50s of 2.1 μM and 1.6 μM, respectively. (S)-(+)-Ibuprofen has analgesic, anti-inflammatory, anticancer and antipyretic effects.

For research use only. We do not sell to patients.

(S)-(+)-Ibuprofen Chemical Structure

(S)-(+)-Ibuprofen Chemical Structure

CAS No. : 51146-56-6

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10 mM * 1 mL in DMSO
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Other Forms of (S)-(+)-Ibuprofen:

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Description

(S)-(+)-Ibuprofen ((S)-Ibuprofen), a S(+)-enantiomer of Ibuprofen, is a potent COX-1 and COX-2 inhibitor with IC50s of 2.1 μM and 1.6 μM, respectively. (S)-(+)-Ibuprofen has analgesic, anti-inflammatory, anticancer and antipyretic effects[1][2].

IC50 & Target[1]

COX-1

2.1 μM (IC50)

COX-2

1.6 μM (IC50)

In Vitro

(S)-(+)-Ibuprofen (HCT-15 and HCA-7 cells; 0-1000 µM; 8 days) treatment reduces concentration dependently cell survival in both cell lines to a similar extent[2].
(S)-(+)-Ibuprofen (HCT-15 and HCA-7 cells; 0-1000 µM; 20-72 hours) treatment causes a G0/G1 phase block as well as apoptosis[2].
(S)-(+)-Ibuprofen (HCT-15 and HCA-7 cells; 900 µM; 4-72 hours) treatment shows a down regulation of cyclin A and B and an increase of the cell cycle inhibitory protein p27Kip-1[2].
(S)-(+)-Ibuprofen inhibits COX activity, thromboxane formation, and platelet aggregation[1].
(S)-(+)-Ibuprofen inhibits the activation of NF-κB in response to T-cell stimulation with an IC50 of 61.7 μM[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Proliferation Assay[2]

Cell Line: HCT-15 and HCA-7 cells
Concentration: 0 µM, 200 µM, 400 µM, 600 µM, 700 µM, 800 µM, 900 µM, and 1000 µM
Incubation Time: 8 days
Result: Reduced concentration dependently cell survival in both cell lines to a similar extent.

Cell Cycle Analysis[2]

Cell Line: HCT-15 and HCA-7 cells
Concentration: 0 µM, 200 µM, 400 µM, 600 µM, 800 µM, 900 µM, and 1000 µM
Incubation Time: 24 hours (HCT-15) or 20 hours (HCA-7)
Result: Caused a G0/G1 phase block.

Apoptosis Analysis[2]

Cell Line: HCT-15 and HCA-7 cells
Concentration: 0 µM, 200 µM, 400 µM, 600 µM, 800 µM, 900 µM, and 1000 µM
Incubation Time: 72 hours
Result: Induced cell apoptosis.

Western Blot Analysis[2]

Cell Line: HCT-15 and HCA-7 cells
Concentration: 900 µM
Incubation Time: 4 hours, 8 hours, 16 hours, 24 hours, 32 hours, 48 hours and 72 hours
Result: Decreased levels of Cyclin D1 protein.
In Vivo

(S)-(+)-Ibuprofen (15 mg/kg/day; intraperitoneal injection; five days a week; for 4 weeks) treatment inhibits tumor growth of HCA-7 and HCT-15 xenografts in the nude mice model[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: NMRI (nu/nu) male mice (6-8 week old ) injected with HCA-7 and HCT-15 cells[2]
Dosage: 15 mg/kg/day
Administration: Intraperitoneal injection; five days a week; for 4 weeks
Result: Inhibited tumor growth of HCA-7 and HCT-15 xenografts in mice.
Clinical Trial
Molecular Weight

206.28

Formula

C13H18O2

CAS No.
Appearance

Solid

Color

White to off-white

SMILES

OC([C@H](C1=CC=C(CC(C)C)C=C1)C)=O

Shipping

Room temperature in continental US; may vary elsewhere.

Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 2 years
-20°C 1 year
Solvent & Solubility
In Vitro: 

DMSO : 100 mg/mL (484.78 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Ethanol : 100 mg/mL (484.78 mM; Need ultrasonic)

H2O : 1 mg/mL (4.85 mM; Need ultrasonic and warming)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 4.8478 mL 24.2389 mL 48.4778 mL
5 mM 0.9696 mL 4.8478 mL 9.6956 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 2.5 mg/mL (12.12 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: ≥ 2.5 mg/mL (12.12 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

    Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.

For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  PBS

    Solubility: 6.67 mg/mL (32.33 mM); Clear solution; Need ultrasonic and warming and heat to 60°C

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
+
%
Tween-80 +
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation

Purity: 99.98%

References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
H2O / DMSO / Ethanol 1 mM 4.8478 mL 24.2389 mL 48.4778 mL 121.1945 mL
DMSO / Ethanol 5 mM 0.9696 mL 4.8478 mL 9.6956 mL 24.2389 mL
10 mM 0.4848 mL 2.4239 mL 4.8478 mL 12.1194 mL
15 mM 0.3232 mL 1.6159 mL 3.2319 mL 8.0796 mL
20 mM 0.2424 mL 1.2119 mL 2.4239 mL 6.0597 mL
25 mM 0.1939 mL 0.9696 mL 1.9391 mL 4.8478 mL
30 mM 0.1616 mL 0.8080 mL 1.6159 mL 4.0398 mL
40 mM 0.1212 mL 0.6060 mL 1.2119 mL 3.0299 mL
50 mM 0.0970 mL 0.4848 mL 0.9696 mL 2.4239 mL
60 mM 0.0808 mL 0.4040 mL 0.8080 mL 2.0199 mL
80 mM 0.0606 mL 0.3030 mL 0.6060 mL 1.5149 mL
100 mM 0.0485 mL 0.2424 mL 0.4848 mL 1.2119 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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