ANO1-IN-5
ANO1-IN-5 is an orally active ANO1 inhibitor with an IC50 of 4.57 μM. J ANO1-IN-5 demonstrates 118-, 642-, and 10000-fold selectivity over α1A-,α1B-, andα1D-AR, respectively. ANO1-IN-5 exhibits significant inhibitory effects on osteoclast differentiation and function. ANO1-IN-5 can used for the study of osteoporosis.
For research use only. We do not sell to patients.
- Formula: C23H33N3O4S
- Molecular Weight:447.59
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
- Protocols
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
Description
In Vitro
ANO1-IN-5 (Compound 10) (20 μM, 5 d) significantly inhibits the formation of TRAP+ multinucleated osteoclasts[1].
ANO1-IN-5 (20 μM) significantly reduces the mRNA expression of osteoclast marker genes such as NFATc1, Acp5, Ctsk, and Mmp9[1].
ANO1-IN-5 directly suppresses the Cl- secretion function of osteoclasts by inhibiting ANO1[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.
Parmacokinetics
In Vivo
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:OVX-induced bone loss model established in 6-8-week-old female mice[1]
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Dosage:3, 10 and 30 mg/kg
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Administration:Intragastrical administration (i.g.), once daily for 4 weeks
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Result:Exhibited higher bone volume/total volume (BV/TV), trabecular number (Tb.N), and trabecular thickness (Tb.Th) values, but a lower trabecular separation (Tb.Sp) in a dose-dependent manner.
Reduced the level of the bone resorption marker CTX-1 in the serum, and downregulated the expression of osteoclast marker genes in the bone tissue.
Chemical Information
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Molecular Weight 447.59
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Formula C23H33N3O4S
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SMILES
O=S(C1=CC(CC(NC2CCN(C3=CC=CC=C3OCC)CC2)C)=CC=C1OC)(N)=O
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Protocols
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Osteoclast differentiation from monocyte/macrophage precursors
Osteoclast differentiation is an in vitro induction assay in which monocyte/macrophage-lineage precursors are exposed to macrophage colony-stimulating factor (M-CSF) and receptor activator of NF-κB ligand (RANKL), generating multinucleated osteoclasts that are commonly identified by tartrate-resistant acid phosphatase (TRAP) staining and functionally confirmed by resorption pits on dentin, bone, or mineralized substrates. M-CSF supports survival and expansion of osteoclast precursors, while RANKL binding to RANK drives osteoclast commitment, fusion, maturation, and resorptive function; osteoprotegerin inhibits this pathway by binding RANKL and preventing RANK activation. The main readouts are the number of TRAP-positive multinucleated cells, formation of F-actin rings, and resorbed surface area; TRAP-positive multinucleated cells indicate osteoclast differentiation, whereas pit formation on dentin, bone, or mineralized coating indicates functional bone-resorbing activity.
Purity & Documentation
References
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
ANO1-IN-5 Related Classifications
HY-175669 Related Classifications
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