Art. -Nr.: HY-B0438

Spectinomycin dihydrochloride

5 Cited Publications

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Spectinomycin dihydrochloride is a broad-spectrum antibiotic and inhibits the growth of a variety of gram-positive and gram-negative organisms. Spectinomycin dihydrochloride acts by selectively targeting to the bacterial ribosome and interrupting protein synthesis. Spectinomycin dihydrochloride is also a noncompetitive inhibitor of td intron RNA with an K_i value of 7.2 mM^-.

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Reinheit: 99.73%
CAS. Nr.: 21736-83-4
Formel: C₁₄H₂₆Cl₂N₂O₇
Molecular Weight:405.27

Speicherung:

4°C, sealed storage, away from moisture

* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Spectinomycin dihydrochloride Chemical Structure

CAS. Nr.: 21736-83-4

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Biologische Aktivität
- Beschreibung
- IC₅₀ & Target
- In Vitro
- In Vivo
Chemical Information
Publications (5)
Lösungsmittel & Löslichkeit
- In Vitro
- In Vivo
Reinheit & Dokumentation
Verweise
Help & FAQs
Classifications
Bioz

Biologische Aktivität

Beschreibung

IC₅₀ & Target

bacterial ribosomal subunit^[5]

Alle Antibiotic Isoform-spezifische Produkte anzeigen :

In Vitro

Spectinomycin dihydrochloride selectively inhibits protein synthesis in cells and in extracts of Escherichia coli:
Spectinomycin dihydrochloride (50 μg/mL) inhibits Escherichia coli growth rapidly and reversibly, and suppresses amino acid incorporation immediately^[1].
Spectinomycin dihydrochloride (1 μg/mL or 3 μM) inhibits polypeptide synthesis directed either by endogenous messenger RNA or by MS-2 bacteriophage RNA, with maximum inhibition of 70-80% in extracts of Escherichia coli^[1]^[1].
Spectinomycin dihydrochloride blocks the translocation of peptidyl-tRNAs from A-site to P-site by inhibiting the binding of elongation factor G to the ribosome^[2].
Spectinomycin dihydrochloride interacts specifically with the residues G1064 and 01192 in 16S rRNA and potentially makes it inactive^[2].
Spectinomycin dihydrochloride exhibits splicing inhibition and dependent on pH changes and Mg²⁺ concentration, indicating electrostatic interactions with the intron RNA^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

Spectinomycin dihydrochloride (20 mg/kg; i.m.; 20-100 mg/kg; 9 d) shows the safety in healthy chicks^[4].
Spectinomycin dihydrochloride (10 mg/kg; i.v.; single dose) has the major elimination pathway by renal excretion, approximately 55% is excreted into the urine in unchanged form^[5].
Pharmacokinetics of Spectinomycin dihydrochloride in Rat^[5]

Parameter C₀ (μg/mL) AUC_0-∞ (μg•h/mL) V_d (L/kg) CL (L/h/kg) MRT (h) T_1/2α (h) T_1/2β (h) T_1/2γ (h) f_e CL_renal (L/h/kg) E_ratio

Non atrioventricular analysis 44.3 16.8 0.756 0.602 0.757 / / / 0.553 0.359 1.00

Three-compartment model 37.8 15.7 0.747 0.649 1.11 / 0.237 0.754 19.5 / /

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:

Arbor Acres plus broiler chicks (15-day-old)^[4]

Dosage:

20 mg/kg, 60 mg/kg, 100 mg/kg

Administration:

Intramuscular injection (chest muscles); 9 days

Result:

Showed biosecurity of 20 mg/kg by complete blood count, biochemical parameters, histopathological, clinical signs, body weight gain, and feed conversion ratio (FCR).
Resulted minor toxicity of 60 mg/kg.

Chemical Information

CAS. Nr. 21736-83-4

Appearance Solid

Molecular Weight 405.27

Formel C₁₄H₂₆Cl₂N₂O₇

Color White to off-white

SMILES

CN[C@@H]([C@H]([C@@H](NC)[C@@H]1O)O)[C@]2([H])[C@]1([H])O[C@@](O[C@H](C)C3)([H])[C@](O)(C3=O)O2.Cl.Cl

Structure Classification

Antibiotics

Disease Research

Antibacterial

Antibiotics

Other Antibiotics

Antibiotics

Mammalian Cell Culture

Antibiotics

Resistance Selection

Initial Source

Microorganisms

bacterium Streptomyces spectabilis

Versand

Room temperature in continental US; may vary elsewhere.

Speicherung

4°C, sealed storage, away from moisture

* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Publications (5)

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Journal Impact Factor

Journal Impact Factor

Most Recent

Nucleic Acids Res

PhoP-regulated VirK acts as an accessory factor to maintain virulence in polymyxin-resistant Klebsiella pneumoniae. [Abstract]

2026 Apr 23;54(8):gkag290. PMID: 42033226

BMC Vet Res

Identification of a broad-spectrum lytic Myoviridae bacteriophage using multidrug resistant Salmonella isolates from pig slaughterhouses as the indicator and its application in combating Salmonella infections. [Abstract]

2022 Jul 12;18(1):270. PMID: 35821025

Vet Microbiol

Discovery of the tigecycline resistance gene cluster tmexCD3-toprJ1 in Pasteurella multocida strains isolated from pigs in China. [Abstract]

2024 May:292:110046. PMID: 38471428

SSRN

2026 May 5.

Patent

US20200368199A1.

Lösungsmittel & Löslichkeit

In Vitro:

H₂O : ≥ 100 mg/mL (246.75 mM)

* "≥" means soluble, but saturation unknown.

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

2.4675 mL

12.3375 mL

24.6749 mL

5 mM

0.4935 mL

2.4675 mL

4.9350 mL

10 mM

0.2467 mL

1.2337 mL

2.4675 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molaritätsrechner

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

=
Konzentration Konzentration

×
Volumen Volumen

×
Molecular Weight Molecular Weight

Verdünnungsrechner

Konzentration _{(Stammlösung)} × Volumen _{(Stammlösung)} = Konzentration _{(Ziellösung)} × Volumen _{(Ziellösung)}

Konzentration _{(Stammlösung)} Konzentration _{(Stammlösung)}

×

Volumen _{(Stammlösung)} Volumen _{(Stammlösung)}

=

Konzentration _{(Ziellösung)} Konzentration _{(Ziellösung)}

×

Volumen _{(Ziellösung)} Volumen _{(Ziellösung)}

In Vivo:

Working Solution

For the following dissolution methods, please prepare the working solution directly:

It is recommended to prepare fresh solutions and use them promptly within a short period of time.

The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: PBS
Solubility: 50 mg/mL (123.37 mM); Clear solution; Need ultrasonic

Reinheit & Dokumentation

Select Batch:

Reinheit: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (297 KB)

SDS (393 KB)

English - EN (393 KB)

Français - FR (393 KB)

Deutsch - DE (393 KB)

Norwegian - NO (393 KB)

Español - ES (393 KB)

Swedish - SV (393 KB)

Italian - IT (393 KB)

Korean - KR (393 KB)

Portuguese - PT (393 KB)

COA (2659 KB)

Elemental Analysis Report (2659 KB)

HNMR (2659 KB)

CNMR (2659 KB)

RP-HPLC (2659 KB)

NP-HPLC (2659 KB)

MS (2659 KB)

LCMS (2659 KB)

IR (2659 KB)

GC (2659 KB)

OR (2659 KB)

SDS-PAGE (2659 KB)

SEC-HPLC (2659 KB)

Other Report (2659 KB)

Handling Instructions (2659 KB)

Verweise

[1]. Davies J, et al. Inhibition of protein synthesis by spectinomycin. Science. 1965 Sep 3;149(3688):1096-8. [Content Brief]

[2]. Brink MF, et al. Spectinomycin interacts specifically with the residues G1064 and C1192 in 16S rRNA, thereby potentially freezing this molecule into an inactive conformation. Nucleic Acids Res. 1994 Feb 11;22(3):325-31. [Content Brief]

[3]. Park IK, et al. Spectinomycin inhibits the self-splicing of the group 1 intron RNA. Biochem Biophys Res Commun. 2000 Mar 16;269(2):574-9. [Content Brief]

[4]. Khan EA, et al. Safety evaluation study of lincomycin and spectinomycin hydrochloride intramuscular injection in chickens. Toxicol Rep. 2022 Jan 29;9:204-209. [Content Brief]

[5]. Madhura DB, et al. Pharmacokinetic profile of spectinomycin in rats. Pharmazie. 2013 Aug;68(8):675-6. [Content Brief]

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Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg

H₂O 1 mM 2.4675 mL 12.3375 mL 24.6749 mL 61.6873 mL

5 mM 0.4935 mL 2.4675 mL 4.9350 mL 12.3375 mL

10 mM 0.2467 mL 1.2337 mL 2.4675 mL 6.1687 mL

15 mM 0.1645 mL 0.8225 mL 1.6450 mL 4.1125 mL

20 mM 0.1234 mL 0.6169 mL 1.2337 mL 3.0844 mL

25 mM 0.0987 mL 0.4935 mL 0.9870 mL 2.4675 mL

30 mM 0.0822 mL 0.4112 mL 0.8225 mL 2.0562 mL

40 mM 0.0617 mL 0.3084 mL 0.6169 mL 1.5422 mL

50 mM 0.0493 mL 0.2467 mL 0.4935 mL 1.2337 mL

60 mM 0.0411 mL 0.2056 mL 0.4112 mL 1.0281 mL

80 mM 0.0308 mL 0.1542 mL 0.3084 mL 0.7711 mL

100 mM 0.0247 mL 0.1234 mL 0.2467 mL 0.6169 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Spectinomycin dihydrochloride Related Classifications

HY-B0438 Related Classifications

Anti-infection

Bacterial Antibiotic

Disease Areas

Infection

Coronavirus Infection Bacterial Infection

SARS-CoV-2 Infection

Natürliche Produkte

Disease Research Fields Antibiotics Microorganisms

Disease Research Infection Other Antibiotics Mammalian Cell Culture Resistance Selection

Antibacterial

Infection

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Keywords

Spectinomycin
21736-83-4
Bacterial
Antibiotic
biosecurity
aminocyclitol
broad-spectrum
gram-positive
gram-negative
organism
bacterial ribosome
Escherichia coli
amino acid incorporation
MS-2 bacteriophage
peptidyl-tRNA
electrostatic interaction
Inhibitor
inhibitor
inhibit

Your Recently Viewed Products

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
H₂O	1 mM	2.4675 mL	12.3375 mL	24.6749 mL	61.6873 mL
5 mM	0.4935 mL	2.4675 mL	4.9350 mL	12.3375 mL
10 mM	0.2467 mL	1.2337 mL	2.4675 mL	6.1687 mL
15 mM	0.1645 mL	0.8225 mL	1.6450 mL	4.1125 mL
20 mM	0.1234 mL	0.6169 mL	1.2337 mL	3.0844 mL
25 mM	0.0987 mL	0.4935 mL	0.9870 mL	2.4675 mL
30 mM	0.0822 mL	0.4112 mL	0.8225 mL	2.0562 mL
40 mM	0.0617 mL	0.3084 mL	0.6169 mL	1.5422 mL
50 mM	0.0493 mL	0.2467 mL	0.4935 mL	1.2337 mL
60 mM	0.0411 mL	0.2056 mL	0.4112 mL	1.0281 mL
80 mM	0.0308 mL	0.1542 mL	0.3084 mL	0.7711 mL
100 mM	0.0247 mL	0.1234 mL	0.2467 mL	0.6169 mL

Spectinomycin dihydrochloride

5 Cited Publications

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Spectinomycin dihydrochloride

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Publications Citing Use of MedChemExpress (MCE) Spectinomycin dihydrochloride

Alle Antibiotic Isoform-spezifische Produkte anzeigen

Biologische Aktivität

Chemical Information

Publications (5)

Lösungsmittel & Löslichkeit

Reinheit & Dokumentation

Verweise

Help & FAQs

Complete Stock Solution Preparation Table

Spectinomycin dihydrochloride Related Classifications

HY-B0438 Related Classifications