Spectinomycin dihydrochloride pentahydrate
Based on 5 publication(s) in Google Scholar
Spectinomycin dihydrochloride pentahydrate is a broad-spectrum antibiotic and inhibits the growth of a variety of gram-positive and gram-negative organisms. Spectinomycin dihydrochloride pentahydrate acts by selectively targeting to the bacterial ribosome and interrupting protein synthesis. Spectinomycin dihydrochloride pentahydrate is also a noncompetitive inhibitor of td intron RNA with an Ki value of 7.2 mM-.
For research use only. We do not sell to patients.
- Purity: 98.0%
- CAS No.: 22189-32-8
- Formula: C14H36Cl2N2O12
- Molecular Weight:495.35
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Storage:
4°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
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Publications Citing Use of MedChemExpress (MCE) Spectinomycin dihydrochloride pentahydrate
MoreAll Antibiotic Isoforms
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Biological Activity
bacterial ribosomal subunit[5]
Spectinomycin dihydrochloride pentahydrate selectively inhibits protein synthesis in cells and in extracts of Escherichia coli:
Spectinomycin dihydrochloride pentahydrate (50 μg/mL) inhibits Escherichia coli growth rapidly and reversibly, and suppresses amino acid incorporation immediately[1].
Spectinomycin dihydrochloride pentahydrate (1 μg/mL or 3 μM) inhibits polypeptide synthesis directed either by endogenous messenger RNA or by MS-2 bacteriophage RNA, with maximum inhibition of 70-80% in extracts of Escherichia coli[1].
Spectinomycin dihydrochloride pentahydrate blocks the translocation of peptidyl-tRNAs from A-site to P-site by inhibiting the binding of elongation factor G to the ribosome[2].
Spectinomycin dihydrochloride pentahydrate interacts specifically with the residues G1064 and 01192 in 16S rRNA and potentially makes it inactive[2].
Spectinomycin dihydrochloride pentahydrate exhibits splicing inhibition and dependent on pH changes and Mg2+ concentration, indicating electrostatic interactions with the intron RNA[3].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Spectinomycin dihydrochloride pentahydrate (10 mg/kg; i.v.; single dose) has the major elimination pathway by renal excretion, approximately 55% is excreted into the urine in unchanged form[5].
Pharmacokinetics of Spectinomycin dihydrochloride pentahydrate in Rat[5]
| Parameter | C0 (μg/mL) | AUC0-∞ (μg•h/mL) | Vd (L/kg) | CL (L/h/kg) | MRT (h) | T1/2α (h) | T1/2β (h) | T1/2γ (h) | fe | CLrenal (L/h/kg) | Eratio |
| Non atrioventricular analysis | 44.3 | 16.8 | 0.756 | 0.602 | 0.757 | / | / | / | 0.553 | 0.359 | 1.00 |
| Three-compartment model | 37.8 | 15.7 | 0.747 | 0.649 | 1.11 | / | 0.237 | 0.754 | 19.5 | / | / |
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:Arbor Acres plus broiler chicks (15-day-old)[4]
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Dosage:20 mg/kg, 60 mg/kg, 100 mg/kg
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Administration:Intramuscular injection (chest muscles); 9 days
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Result:Showed biosecurity of 20 mg/kg by complete blood count, biochemical parameters, histopathological, clinical signs, body weight gain, and feed conversion ratio (FCR).
Resulted minor toxicity of 60 mg/kg.
Chemical Information
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CAS No. 22189-32-8
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Appearance Solid
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Molecular Weight 495.35
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Formula C14H36Cl2N2O12
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Color White to off-white
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SMILES
O=C1C[C@@H](C)O[C@@]2([H])O[C@@]3([H])[C@]([C@@H](NC)[C@@H](O)[C@@H](NC)[C@@H]3O)([H])O[C@]21O.[H]Cl.[H]Cl.[5H2O]
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Synonyms
Spectinomycin hydrochloride hydrate
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
4°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
Publications (5)
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Journal Impact Factor
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Most Recent
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Nucleic Acids Res
PhoP-regulated VirK acts as an accessory factor to maintain virulence in polymyxin-resistant Klebsiella pneumoniae. [Abstract]2026 Apr 23;54(8):gkag290. PMID: 42033226 -
BMC Vet Res
Identification of a broad-spectrum lytic Myoviridae bacteriophage using multidrug resistant Salmonella isolates from pig slaughterhouses as the indicator and its application in combating Salmonella infections. [Abstract]2022 Jul 12;18(1):270. PMID: 35821025 -
Vet Microbiol
Discovery of the tigecycline resistance gene cluster tmexCD3-toprJ1 in Pasteurella multocida strains isolated from pigs in China. [Abstract]2024 May:292:110046. PMID: 38471428 -
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Solvent & Solubility
H2O : 62.5 mg/mL (126.17 mM; Need ultrasonic)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
For the following dissolution methods, please prepare the working solution directly:
It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: PBS
Solubility: 50 mg/mL (100.94 mM); Clear solution; Need ultrasonic
Purity & Documentation
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Data Sheet (298 KB)
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SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
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Handling Instructions (2659 KB)
References
[1]. Davies J, et al. Inhibition of protein synthesis by spectinomycin. Science. 1965 Sep 3;149(3688):1096-8. [Content Brief]
[2]. Brink MF, et al. Spectinomycin interacts specifically with the residues G1064 and C1192 in 16S rRNA, thereby potentially freezing this molecule into an inactive conformation. Nucleic Acids Res. 1994 Feb 11;22(3):325-31. [Content Brief]
[3]. Park IK, et al. Spectinomycin inhibits the self-splicing of the group 1 intron RNA. Biochem Biophys Res Commun. 2000 Mar 16;269(2):574-9. [Content Brief]
[4]. Madhura DB, et al. Pharmacokinetic profile of spectinomycin in rats. Pharmazie. 2013 Aug;68(8):675-6. [Content Brief]
[5]. Khan EA, et al. Safety evaluation study of lincomycin and spectinomycin hydrochloride intramuscular injection in chickens. Toxicol Rep. 2022 Jan 29;9:204-209. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| H2O | 1 mM | 2.0188 mL | 10.0939 mL | 20.1877 mL | 50.4694 mL |
| 5 mM | 0.4038 mL | 2.0188 mL | 4.0375 mL | 10.0939 mL | |
| 10 mM | 0.2019 mL | 1.0094 mL | 2.0188 mL | 5.0469 mL | |
| 15 mM | 0.1346 mL | 0.6729 mL | 1.3458 mL | 3.3646 mL | |
| 20 mM | 0.1009 mL | 0.5047 mL | 1.0094 mL | 2.5235 mL | |
| 25 mM | 0.0808 mL | 0.4038 mL | 0.8075 mL | 2.0188 mL | |
| 30 mM | 0.0673 mL | 0.3365 mL | 0.6729 mL | 1.6823 mL | |
| 40 mM | 0.0505 mL | 0.2523 mL | 0.5047 mL | 1.2617 mL | |
| 50 mM | 0.0404 mL | 0.2019 mL | 0.4038 mL | 1.0094 mL | |
| 60 mM | 0.0336 mL | 0.1682 mL | 0.3365 mL | 0.8412 mL | |
| 80 mM | 0.0252 mL | 0.1262 mL | 0.2523 mL | 0.6309 mL | |
| 100 mM | 0.0202 mL | 0.1009 mL | 0.2019 mL | 0.5047 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Spectinomycin dihydrochloride pentahydrate Related Classifications
HY-B1828A Related Classifications
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