Art. -Nr.: HY-128140

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Z-FY-CHO

(Synonyms: Z-Phe-Tyr-CHO)

8 Cited Publications

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Based on 8 publication(s) in Google Scholar

Z-FY-CHO (Z-Phe-Tyr-CHO) is a potent and specific cathepsin L (CTSL) inhibitor.

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Reinheit: 95.0%
CAS. Nr.: 167498-29-5
Formel: C₂₆H₂₆N₂O₅
Molecular Weight:446.50

Speicherung:

-20°C, sealed storage, away from moisture

* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Z-FY-CHO Chemical Structure

CAS. Nr.: 167498-29-5

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Biologische Aktivität
- Beschreibung
- IC₅₀ & Target
- Cellular Effect
- In Vitro
- In Vivo
Chemical Information
Publications (8)
Lösungsmittel & Löslichkeit
- In Vitro
- In Vivo
Reinheit & Dokumentation
Verweise
Help & FAQs
Classifications
Bioz

Biologische Aktivität

Beschreibung

Z-FY-CHO (Z-Phe-Tyr-CHO) is a potent and specific cathepsin L (CTSL) inhibitor^[1]^[2].

IC₅₀ & Target

cathepsin L

Alle Cathepsin Isoform-spezifische Produkte anzeigen :

Cellular Effect

Cell Line	Type	Value	Description	References
HL-60	ED₅₀	21500 nM Compound: 10a	Cytotoxicity against human HL60 cells Cytotoxicity against human HL60 cells	[PMID: 23611656]

In Vitro

Z-FY-CHO (10 μM, for 1 h) pretreatment alleviated cell death induced by 6-OHDA. Z-FY-CHO inhibits the increase in CTSL protein expression in 6-OHDA-treated SH-SY5Y cells. Treatment with Z-FY-CHO caused more LC3-II and less P62 expression in SH-SY5Y cells treated with 6-OHDA, indicating enhancing autophagy activity, and Z-FY-CHO blocks activation of caspase-3 and PARP^[1].
Treatment with the specific cathepsin L inhibitor Z-FY-CHO (10 μM) or transfection with cathepsin L shRNA significantly increased the radiosensitivity of U251 cells. Both suppression and knockdown of cathepsin L in U251 cells increased irradiation-induced DNA damage and G2/M phase cell cycle arrest. Both suppression and knockdown of cathepsin L in U251 cells also increased irradiation-induced apoptosis, as shown by the increased levels of Bax and decreased levels of Bcl-2^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

Intraperitoneal administration of Z-FY-CHO (2.5-10 mg/kg) for 4 weeks suppresses bone weight loss dose dependently in the ovariectomized mouse, experimental model of osteoporosis. Z-FY-CHO acts as a bone resorption suppressor through the inhibition of collagen degradation^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Chemical Information

CAS. Nr. 167498-29-5
Appearance Solid
Molecular Weight 446.50
Formel C₂₆H₂₆N₂O₅
Color White to off-white
SMILES

O=C(OCC1=CC=CC=C1)N[C@@H](CC2=CC=CC=C2)C(N[C@H](C=O)CC3=CC=C(O)C=C3)=O
Synonyms

Z-Phe-Tyr-CHO
Versand

Room temperature in continental US; may vary elsewhere.
Speicherung

-20°C, sealed storage, away from moisture

* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Publications (8)

All Cell Proliferation/Viability AssayCell Imaging/StainingRT-PCRBio/Physico-chemical Assay

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Journal Impact Factor

Mol Cancer

Sulindac (K-80003) with nab-paclitaxel and gemcitabine overcomes drug-resistant pancreatic cancer. [Abstract]

2024 Sep 30;23(1):215. PMID: 39350121
Cell Discov

Single-cell transcriptome reveals cellular hierarchies and guides p-EMT-targeted trial in skull base chordoma. [Abstract]

2022 Sep 20;8(1):94. PMID: 36127333
Cell Death Dis

Prosapogenin A induces GSDME-dependent pyroptosis of anaplastic thyroid cancer through vacuolar ATPase activation-mediated lysosomal over-acidification. [Abstract]

2024 Aug 13;15(8):586. PMID: 39138191
Proc Natl Acad Sci U S A

TMPRSS2-mediated SARS-CoV-2 uptake boosts innate immune activation, enhances cytopathology, and drives convergent virus evolution. [Abstract]

2024 Jun 4;121(23):e2407437121. PMID: 38814864
Basic Res Cardiol

Antimicrobial peptide CRAMP/LL-37 mediates ferroptosis resistance in cardiomyocytes by inhibiting cathepsin L. [Abstract]

2025 Jun 15. PMID: 40517353
Emerg Microbes Infect

Porcine deltacoronavirus resists antibody neutralization through cell-to-cell transmission. [Abstract]

2023 Dec;12(1):2207688. PMID: 37125733

Z-FY-CHO purchased from MedChemExpress. Usage Cited in: Emerg Microbes Infect. 2023 Dec;12(1):2207688. [Abstract]

IPI-2I cells cultured in 96-well plates were incubated with leupeptin (50 µM), Baf-A1 (50 nM), E64d (25 µM), Z-FY-CHO (20 µM), or CA-074 (5 µM). After 24 h incubation, 10 μl of CCK-8 was added to the cells and incubated for 1 h at 37°C, followed by measurement of OD value at 450 nm.

Z-FY-CHO purchased from MedChemExpress. Usage Cited in: Emerg Microbes Infect. 2023 Dec;12(1):2207688. [Abstract]

The results showed that leupeptin, Baf-A1, E64d, and Z-FY-CHO significantly reduced the cell–cell fusion induced by PDCoV.
J Virol

Cathepsin L and transmembrane serine protease 11E mediate trypsin-independent entry of porcine deltacoronavirus into Huh7 cells. [Abstract]

2025 Aug 11:e0105525. PMID: 40787987

Z-FY-CHO purchased from MedChemExpress. Usage Cited in: J Virol. 2025 Aug 11:e0105525. [Abstract]

Effect of Z-FY-CHO on PDCoV infection. Huh7 cells were pretreated with different concentrations of Z-FY-CHO (0, 12.5, 25 and 50 µM), and then the cells were infected with PDCoV^T- (MOI = 0.5). At 24 hpi, the viral RNA copies and titers were measured by RT-qPCR.

Z-FY-CHO purchased from MedChemExpress. Usage Cited in: J Virol. 2025 Aug 11:e0105525. [Abstract]

Effect of Z-FY-CHO on PDCoV infection. Huh7 cells were pretreated with different concentrations of Z-FY-CHO (0, 12.5, 25 and 50 µM), and then the cells were infected with PDCoV^T- (MOI = 0.5). At 24 hpi, the viral RNA copies and titers were measured by TCID₅₀ assay.

Z-FY-CHO purchased from MedChemExpress. Usage Cited in: J Virol. 2025 Aug 11:e0105525. [Abstract]

Viral internalization assay with Z-FY-CHO. Huh7 cells were pretreated with different concentrations of Z-FY-CHO (0, 12.5, 25, and 50 µM), then the viral internalization assay was performed with PDCoV^T- (MOI = 1), and the internalized viral RNA copies were quantified by RT-qPCR.
Res Sq

2025 Sep 7.

Lösungsmittel & Löslichkeit

In Vitro:

DMSO : 100 mg/mL (223.96 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Ethanol : 10 mg/mL (22.40 mM; Need ultrasonic)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

2.2396 mL

11.1982 mL

22.3964 mL

5 mM

0.4479 mL

2.2396 mL

4.4793 mL

10 mM

0.2240 mL

1.1198 mL

2.2396 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molaritätsrechner

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Konzentration Konzentration

Volumen Volumen

Molecular Weight Molecular Weight

Verdünnungsrechner

Konzentration _{(Stammlösung)} × Volumen _{(Stammlösung)} = Konzentration _{(Ziellösung)} × Volumen _{(Ziellösung)}

Konzentration _{(Stammlösung)} Konzentration _{(Stammlösung)}

Volumen _{(Stammlösung)} Volumen _{(Stammlösung)}

Konzentration _{(Ziellösung)} Konzentration _{(Ziellösung)}

Volumen _{(Ziellösung)} Volumen _{(Ziellösung)}

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline

Solubility: ≥ 1.25 mg/mL (2.80 mM); Clear solution

This protocol yields a clear solution of ≥ 1.25 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (12.5 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)

Solubility: 1.25 mg/mL (2.80 mM); Suspended solution; Need ultrasonic

This protocol yields a suspended solution of 1.25 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (12.5 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil

Solubility: ≥ 1.25 mg/mL (2.80 mM); Clear solution

This protocol yields a clear solution of ≥ 1.25 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (12.5 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

%
DMSO +

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
%
+
+
%
Tween-80 + +
%
Saline +

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Working solution concentration: 0.22 mg/mL

Reinheit & Dokumentation

Select Batch:

Reinheit: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (275 KB)
SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

Verweise

[1]. Lingyun Li, et al. Activated cathepsin L is associated with the switch from autophagy to apoptotic death of SH-SY5Y cells exposed to 6-hydroxydopamine. Biochem Biophys Res Commun. 2016 Feb 12;470(3):579-585. [Content Brief]

[2]. Qing-qing Zhang, et al. Cathepsin L suppression increases the radiosensitivity of human glioma U251 cells via G2/M cell cycle arrest and DNA damage. Acta Pharmacol Sin. 2015 Sep;36(9):1113-25. [Content Brief]

[3]. J T Woo, et al. Suppressive effect of N-(benzyloxycarbonyl)-L-phenylalanyl-L-tyrosinal on bone resorption in vitro and in vivo. Eur J Pharmacol. 1996 Apr 4;300(1-2):131-5. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
Ethanol / DMSO	1 mM	2.2396 mL	11.1982 mL	22.3964 mL	55.9910 mL
	5 mM	0.4479 mL	2.2396 mL	4.4793 mL	11.1982 mL
	10 mM	0.2240 mL	1.1198 mL	2.2396 mL	5.5991 mL
	15 mM	0.1493 mL	0.7465 mL	1.4931 mL	3.7327 mL
	20 mM	0.1120 mL	0.5599 mL	1.1198 mL	2.7996 mL
DMSO	25 mM	0.0896 mL	0.4479 mL	0.8959 mL	2.2396 mL
	30 mM	0.0747 mL	0.3733 mL	0.7465 mL	1.8664 mL
	40 mM	0.0560 mL	0.2800 mL	0.5599 mL	1.3998 mL
	50 mM	0.0448 mL	0.2240 mL	0.4479 mL	1.1198 mL
	60 mM	0.0373 mL	0.1866 mL	0.3733 mL	0.9332 mL
	80 mM	0.0280 mL	0.1400 mL	0.2800 mL	0.6999 mL
	100 mM	0.0224 mL	0.1120 mL	0.2240 mL	0.5599 mL

Keywords

Z-FY-CHO

8 Cited Publications

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Z-FY-CHO

Metabolism/Protease Compound Library

Covalent Screening Library

Angiogenesis-Related Compound Library

Protease Inhibitor Library

Cysteine Targeted Covalent Library

Highly Selective Inhibitors Library

Bioactive Compound Library Max

MCE Bioactive Compound Library

Covalent Screening Library Plus

Bioactive Compound Library

High-Throughput Bioactive Compound Library

Publications Citing Use of MedChemExpress (MCE) Z-FY-CHO

Alle Cathepsin Isoform-spezifische Produkte anzeigen

Biologische Aktivität

Chemical Information

Publications (8)

Lösungsmittel & Löslichkeit

Reinheit & Dokumentation

Verweise

Help & FAQs

Complete Stock Solution Preparation Table

Z-FY-CHO

Metabolism/Protease Compound Library

Covalent Screening Library

Angiogenesis-Related Compound Library

Protease Inhibitor Library

Cysteine Targeted Covalent Library

Highly Selective Inhibitors Library

Bioactive Compound Library Max

MCE Bioactive Compound Library

Covalent Screening Library Plus

Bioactive Compound Library

High-Throughput Bioactive Compound Library

Publications Citing Use of MedChemExpress (MCE) Z-FY-CHO

Alle Cathepsin Isoform-spezifische Produkte anzeigen

Biologische Aktivität

Chemical Information

Publications (8)

Lösungsmittel & Löslichkeit

Reinheit & Dokumentation

Verweise

Help & FAQs

Complete Stock Solution Preparation Table

Z-FY-CHO Related Classifications

HY-128140 Related Classifications