Dibutyl phthalate
Based on 11 publication(s) in Google Scholar
Dibutyl phthalate is a commonly used plasticizer commonly found in some food packaging materials, personal care products, and the coating of oral medications. May cause toxicity and adverse neurobehavioral effects.
For research use only. We do not sell to patients.
- Purity: 99.85%
- CAS No.: 84-74-2
- Formula: C16H22O4
- Molecular Weight:278.34
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Storage:Pure form -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
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Publications Citing Use of MedChemExpress (MCE) Dibutyl phthalate
More- Nat Commun. 2024 Sep 19;15(1):8221. [Abstract]
- J Adv Res. 2025 Apr 3:S2090-1232(25)00220-6. [Abstract]
- Environ Pollut. 2026 Jun 1:398:128010. [Abstract]
- Environ Pollut. 2025 May 7:126395. [Abstract]
- J Agric Food Chem. 2025 Jul 30;73(30):18804-18821. [Abstract]
- Ecotoxicol Environ Saf. 2026 Apr 1:314:120051. [Abstract]
- Ecotoxicol Environ Saf. 2025 May 28:299:118398. [Abstract]
- iScience. 2026 Jan 5;29(2):114619. [Abstract]
- Food Chem Toxicol. 2026 Jul:213:116091. [Abstract]
- Food Chem Toxicol. 2026 Jun:212:116039. [Abstract]
- Sci Total Environ. 2023 Nov 1:897:165500. [Abstract]
Customer Validation & Images
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In Vivo Efficacy Study
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In Vivo Efficacy Study
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IHC
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Bio/Physico-chemical Assay
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IHC
Biological Activity
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Cell Line
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Type | Value | Description | References |
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| B16-F10 | IC50 |
0.23 mM
Compound: 1
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Inhibition of cathepsin B in mouse B16F10 cells
Inhibition of cathepsin B in mouse B16F10 cells
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[PMID: 18289850] |
Dibutyl phthalate (0.001 μg/mL-1000 μg/mL) is detrimental to follicle growth and viability and results in significant dysregulation of cell cycle and apoptosis gene expression in a dose-specific manner. But MBP does not play a role in Dibutyl phthalate toxicity in follicles exposed in vitro[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Dibutyl phthalate (6.25, 12.5, 25, 50, 100 and 200 mg/kg) could cause some neurobehavioral adverse effects in mice[3].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 84-74-2
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Appearance Liquid (Density: 1.043 g/cm3 )
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Molecular Weight 278.34
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Formula C16H22O4
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Color Colorless to light yellow
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SMILES
O=C(C1=CC=CC=C1C(OCCCC)=O)OCCCC
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Structure Classification
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Initial Source
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Pure form -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Publications (11)
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Journal Impact Factor
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Most Recent
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Nat Commun
2024 Sep 19;15(1):8221. PMID: 39300135 -
J Adv Res
Natural, safety immunomodulatory derivatives of lactobacillus biofilms promote diabetic wound healing by metabolically regulating macrophage phenotype and alleviating local inflammation. [Abstract]2025 Apr 3:S2090-1232(25)00220-6. PMID: 40187726 -
Environ Pollut
Dibutyl phthalate exposure-induced AhR activation drives ferroptosis and HMGB1/TLR4-mediated inflammatory liver injury via NRF2-HO-1 signaling. [Abstract]2026 Jun 1:398:128010. PMID: 41887355 -
Environ Pollut
Novel insights into DBP-induced zebrafish liver inflammatory damage: Ferroptosis activating the HMGB1-TLR4-NF-κB signaling pathway. [Abstract]2025 May 7:126395. PMID: 40345373 -
J Agric Food Chem
Analysis of the Antagonistic Effect of Dibutyl Phthalate on Magnaporthe oryzae through Bioassay and Omics Data. [Abstract]2025 Jul 30;73(30):18804-18821. PMID: 40674033 -
Ecotoxicol Environ Saf
The role of Lactobacillus in dibutyl phthalate-induced gut and liver injury: Probiotic intervention and multi-omics evidence. [Abstract]2026 Apr 1:314:120051. PMID: 41895142
Dibutyl phthalate purchased from MedChemExpress. Usage Cited in: Ecotoxicol Environ Saf. 2026 Apr 1:314:120051. [Abstract]
Mice in the Dibutyl phthalate (DBP, 50-200 mg/kg, 28 days)-treated group displayed lethargy, disheveled coats, and sluggish responses. Adverse reactions intensified with increasing DBP doses, though no mouse deaths occurred during the trial.
Dibutyl phthalate purchased from MedChemExpress. Usage Cited in: Ecotoxicol Environ Saf. 2026 Apr 1:314:120051. [Abstract]
With increasing Dibutyl phthalate (DBP, 50-200 mg/kg, 28 days) doses, mouse body weight gradually decreased while liver weight and organ coefficient progressively increased.
Dibutyl phthalate purchased from MedChemExpress. Usage Cited in: Ecotoxicol Environ Saf. 2026 Apr 1:314:120051. [Abstract]
Histopathological analysis revealed that Dibutyl phthalate (DBP, 50-200 mg/kg, 28 days) exposure caused severe liver damage characterized by disrupted hepatic cord arrangement, hepatocyte swelling, and the appearance of lipid droplets.
Dibutyl phthalate purchased from MedChemExpress. Usage Cited in: Ecotoxicol Environ Saf. 2026 Apr 1:314:120051. [Abstract]
Dibutyl phthalate (DBP) exposure induced differential expression in 454 genes, with 240 up-regulated and 214 down-regulated genes identified.
Dibutyl phthalate purchased from MedChemExpress. Usage Cited in: Ecotoxicol Environ Saf. 2026 Apr 1:314:120051. [Abstract]
Dibutyl phthalate (DBP, 50-200 mg/kg, 28 days) exposure induced severe damage to the intestinal physical barrier. Histological examination showed shortened and disordered ileal villi.
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Ecotoxicol Environ Saf
Subacute dibutyl phthalate exposure impairs hepatic T cells and facilitates hepatitis B progression in mouse liver. [Abstract]2025 May 28:299:118398. PMID: 40440982 -
iScience
Mechanistic evidence for dibutyl phthalate as an environmental trigger for inflammatory bowel disease. [Abstract]2026 Jan 5;29(2):114619. PMID: 41630903 -
Food Chem Toxicol
Integrative network toxicology reveals lipoprotein lipase as a key mediator of dibutyl phthalate-associated head and neck squamous cell carcinoma. [Abstract]2026 Jul:213:116091. PMID: 41935815 -
Food Chem Toxicol
Integrated network toxicology and transcriptomics analysis reveals the pathogenic mechanisms in DBP/MBP-induced MASLD/MASH. [Abstract]2026 Jun:212:116039. PMID: 41771476 -
Sci Total Environ
2023 Nov 1:897:165500. PMID: 37442457
Solvent & Solubility
DMSO : 100 mg/mL (359.27 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Ethanol : ≥ 50 mg/mL (179.64 mM)
* "≥" means soluble, but saturation unknown.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (8.98 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (8.98 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Add each solvent one by one: 10% EtOH 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (8.98 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL EtOH stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% EtOH 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (8.98 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL EtOH stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Add each solvent one by one: 10% EtOH 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (8.98 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL EtOH stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.
For the following dissolution methods, please prepare the working solution directly:
It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: Corn Oil
Solubility: ≥ 100 mg/mL (359.27 mM); Clear solution
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
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Data Sheet (276 KB)
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SDS (622 KB)
- English - EN (622 KB)
- Français - FR (622 KB)
- Deutsch - DE (622 KB)
- Norwegian - NO (622 KB)
- Español - ES (622 KB)
- Swedish - SV (622 KB)
- Italian - IT (622 KB)
- Korean - KR (622 KB)
- Portuguese - PT (622 KB)
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Handling Instructions (2659 KB)
References
[1]. Rasmussen LM, et al. Effects of in vitro exposure to dibutyl phthalate, mono-butyl phthalate, and acetyl tributyl citrate on ovarian antral follicle growth and viability. Biol Reprod. 2017 May 1;96(5):1105-1117. [Content Brief]
[2]. Aly HA, et al. Dibutyl phthalate induces oxidative stress and impairs spermatogenesis in adult rats. Toxicol Ind Health. 2016 Aug;32(8):1467-1477. [Content Brief]
[3]. Farzanehfar V, et al. Determination of dibutyl phthalate neurobehavioral toxicity in mice. Food Chem Toxicol. 2016 Aug;94:221-6. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| Ethanol / DMSO | 1 mM | 3.5927 mL | 17.9636 mL | 35.9273 mL | 89.8182 mL |
| 5 mM | 0.7185 mL | 3.5927 mL | 7.1855 mL | 17.9636 mL | |
| 10 mM | 0.3593 mL | 1.7964 mL | 3.5927 mL | 8.9818 mL | |
| 15 mM | 0.2395 mL | 1.1976 mL | 2.3952 mL | 5.9879 mL | |
| 20 mM | 0.1796 mL | 0.8982 mL | 1.7964 mL | 4.4909 mL | |
| 25 mM | 0.1437 mL | 0.7185 mL | 1.4371 mL | 3.5927 mL | |
| 30 mM | 0.1198 mL | 0.5988 mL | 1.1976 mL | 2.9939 mL | |
| 40 mM | 0.0898 mL | 0.4491 mL | 0.8982 mL | 2.2455 mL | |
| 50 mM | 0.0719 mL | 0.3593 mL | 0.7185 mL | 1.7964 mL | |
| 60 mM | 0.0599 mL | 0.2994 mL | 0.5988 mL | 1.4970 mL | |
| 80 mM | 0.0449 mL | 0.2245 mL | 0.4491 mL | 1.1227 mL | |
| 100 mM | 0.0359 mL | 0.1796 mL | 0.3593 mL | 0.8982 mL |
Dibutyl phthalate Related Classifications
HY-Y0304 Related Classifications
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