2. Metabolic Enzyme/Protease
  3. Endogenous Metabolite
  4. Mono-(2-ethylhexyl) phthalate

Mono-(2-ethylhexyl) phthalate  (Synonyms: MEHP; Phthalic acid mono-2-ethylhexyl ester)

Cat. No.: HY-W018392 Purity: 97.70%
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Mono-(2-ethylhexyl) phthalate (MEHP) is a major bioactive metabolite of diethylhexyl phthalate (DEHP). Mono-(2-ethylhexyl) phthalate can promote fatty acid synthesis in hepatocytes by regulating the expression of relevant genes and proteins, contributing to non-alcoholic fatty liver disease (NAFLD).

For research use only. We do not sell to patients.

CAS No. : 4376-20-9

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Based on 22 publication(s) in Google Scholar

Other Forms of Mono-(2-ethylhexyl) phthalate:

Mono-(2-ethylhexyl) phthalate Related Antibodies

Top Publications Citing Use of Products

    Mono-(2-ethylhexyl) phthalate purchased from MedChemExpress. Usage Cited in: J Cachexia Sarcopenia Muscle. 2022 Dec;13(6):2740-2751.  [Abstract]

    Treatment with 250 μM Mono-(2-ethylhexyl) phthalate for 3 days reduced the viability and morphology of C2C12 cells.

    Mono-(2-ethylhexyl) phthalate purchased from MedChemExpress. Usage Cited in: J Cachexia Sarcopenia Muscle. 2022 Dec;13(6):2740-2751.  [Abstract]

    Mono-(2-ethylhexyl) phthalate (250 μM, 48 h) treatment promoted C2C12 cell apoptosis.

    Mono-(2-ethylhexyl) phthalate purchased from MedChemExpress. Usage Cited in: J Cachexia Sarcopenia Muscle. 2022 Dec;13(6):2740-2751.  [Abstract]

    Western blot analyses revealed that Mono-(2-ethylhexyl) phthalate (0-1000 μM, 48 h) treatment up-regulated caspase-3 cleavage and Myostatin expression in C2C12 cells in a dose-dependent manner.

    Mono-(2-ethylhexyl) phthalate purchased from MedChemExpress. Usage Cited in: J Cachexia Sarcopenia Muscle. 2022 Dec;13(6):2740-2751.  [Abstract]

    RT-qPCR indicated that C/EBPδ silencing abrogated the Mono-(2-ethylhexyl) phthalate (48 h)-regulated Myogenin, MyoD, Myostatin, MuRF-1, and Atrogin-1 expression in C2C12 cells.

    Mono-(2-ethylhexyl) phthalate purchased from MedChemExpress. Usage Cited in: J Cachexia Sarcopenia Muscle. 2022 Dec;13(6):2740-2751.  [Abstract]

    Luciferase assay indicated that Mono-(2-ethylhexyl) phthalate (125 and 500 μM, 48 h) enhanced the transcription activity of the myostatin promoter.

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    Human Endogenous Metabolite Microbial Metabolite

    • Biological Activity

    • Purity & Documentation

    • References

    • Customer Review

    Description

    Mono-(2-ethylhexyl) phthalate (MEHP) is a major bioactive metabolite of diethylhexyl phthalate (DEHP). Mono-(2-ethylhexyl) phthalate can promote fatty acid synthesis in hepatocytes by regulating the expression of relevant genes and proteins, contributing to non-alcoholic fatty liver disease (NAFLD)[1].

    IC50 & Target

    Human Endogenous Metabolite

     

    In Vitro

    Mono-(2-ethylhexyl) phthalate (0-100 μM, 48 h) can promote lipid accumulation in HepG2 cells[1].
    Mono-(2-ethylhexyl) phthalate (0-100 μM, 24-48 h) increases the TG level in HepG2 cells in a dose-dependent manner[1].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Mono-(2-ethylhexyl) phthalate Related Antibodies

    Cell Viability Assay[1]

    Cell Line: HepG2 cells
    Concentration: 0-250 μM
    Incubation Time: 24-72 h
    Result: Decreased cell viability at 50-250 μM.

    Western Blot Analysis[1]

    Cell Line: HepG2 cells
    Concentration: 0-100 μM
    Incubation Time: 6-48 h
    Result: Increased the levels of ACC1, FASN, and SCD proteins after (24 h).
    Decreased the levels of ACC1, FASN, and SCD proteins (48 h).
    Increased the levels of ChREBP (24-48 h).
    Increased the expressions of five genes (SREBP-1c, ACC1, FASN, SCD and ChREBP) within a short period of time (6 h), and decreased with prolonged exposure (24 and 48 h).
    In Vivo

    Mono-(2-ethylhexyl) phthalate (0.1-1 mL/kg, p.o., daily, 3 days) causes fetal malformations and reduces live fetuses in pregnant mice[2].
    Mono-(2-ethylhexyl) phthalate (25-50 mg/kg, i.p., once time) induces a significantly high incidence of somatic mutations in the coat hair of offspring of mice[2].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
    Molecular Weight

    278.34

    Formula

    C16H22O4
    CAS No.
    Appearance

    <25°C Solid,>27°C Liquid

    Color

    Colorless to off-white

    SMILES

    O=C(O)C1=CC=CC=C1C(OCC(CC)CCCC)=O
    Structure Classification
    Initial Source
    Shipping

    Room temperature in continental US; may vary elsewhere.
    Storage
    Pure form -20°C 3 years
    4°C 2 years
    In solvent -80°C 1 year
    -20°C 6 months
    Solvent & Solubility
    In Vitro: 

    DMSO : ≥ 100 mg/mL (359.27 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

    *"≥" means soluble, but saturation unknown.

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 3.5927 mL 17.9636 mL 35.9273 mL
    5 mM 0.7185 mL 3.5927 mL 7.1855 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.

    • Molarity Calculator

    • Dilution Calculator

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    Mass
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    Concentration
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    Volume
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    Molecular Weight *

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    Concentration (start)

    C1

    ×
    Volume (start)

    V1

    =
    Concentration (final)

    C2

    ×
    Volume (final)

    V2

    In Vivo:

    Select the appropriate dissolution method based on your experimental animal and administration route.

    For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
    To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

      Solubility: ≥ 2.5 mg/mL (8.98 mM); Clear solution

      This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

      Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
    • Protocol 2

      Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

      Solubility: ≥ 2.5 mg/mL (8.98 mM); Clear solution

      This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

      Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.

    For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  PBS

      Solubility: 4.29 mg/mL (15.41 mM); Clear solution; Need ultrasonic

    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

    Dosage

    mg/kg

    Animal weight
    (per animal)

    g

    Dosing volume
    (per animal)

    μL

    Number of animals

    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Please enter your animal formula composition:
    %
    DMSO +
    % 
    +
    %
    Tween-80 +
    %
    Saline
    Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
    The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
    Calculation results:
    Working solution concentration: mg/mL
    Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
    The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
    Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
     If the continuous dosing period exceeds half a month, please choose this protocol carefully.
    Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
    Purity & Documentation

    Purity: 97.70%

    SDS (394 KB)

    COA (246 KB) HNMR (261 KB) LCMS (95 KB)

    Handling Instructions (2659 KB)
    References

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    DMSO 1 mM 3.5927 mL 17.9636 mL 35.9273 mL 89.8182 mL
    5 mM 0.7185 mL 3.5927 mL 7.1855 mL 17.9636 mL
    10 mM 0.3593 mL 1.7964 mL 3.5927 mL 8.9818 mL
    15 mM 0.2395 mL 1.1976 mL 2.3952 mL 5.9879 mL
    20 mM 0.1796 mL 0.8982 mL 1.7964 mL 4.4909 mL
    25 mM 0.1437 mL 0.7185 mL 1.4371 mL 3.5927 mL
    30 mM 0.1198 mL 0.5988 mL 1.1976 mL 2.9939 mL
    40 mM 0.0898 mL 0.4491 mL 0.8982 mL 2.2455 mL
    50 mM 0.0719 mL 0.3593 mL 0.7185 mL 1.7964 mL
    60 mM 0.0599 mL 0.2994 mL 0.5988 mL 1.4970 mL
    80 mM 0.0449 mL 0.2245 mL 0.4491 mL 1.1227 mL
    100 mM 0.0359 mL 0.1796 mL 0.3593 mL 0.8982 mL
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    Mono-(2-ethylhexyl) phthalate Related Classifications

    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    Mono-(2-ethylhexyl) phthalate4376-20-9MEHP Phthalic acid mono-2-ethylhexyl esterEndogenous MetaboliteDEHPNAFLDfatty acidhepatocytesInhibitorinhibitorinhibit

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