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  3. Environmental Pollutants Endogenous Metabolite
  4. DEHP

DEHP  (Synonyms: Bis(2-ethylhexyl) phthalate; Ergoplast FDO; ESBO-D 82)

Cat. No.: HY-B1945 Purity: 99.56%
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Handling Instructions Technical Support

DEHP (Bis(2-ethylhexyl) phthalate) is a widely used plasticizer, which has orally active. DEHP can produce a wide spectrum of toxic effects on organisms including neurotoxicity, liver toxicity, immunotoxicity, and reproductive toxicity.

For research use only. We do not sell to patients.

CAS No. : 117-81-7

Size Price Stock Quantity
Liquid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
 In-stock
Solution
10 mM * 1 mL in DMSO In-stock
Liquid
100 mg In-stock
200 mg   Get quote  
500 mg   Get quote  

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This product is a controlled substance and not for sale in your territory.

Customer Review

Based on 10 publication(s) in Google Scholar

Other Forms of DEHP:

DEHP Related Antibodies

Top Publications Citing Use of Products

    DEHP purchased from MedChemExpress. Usage Cited in: J Hazard Mater. 2025 Sep 5:495:139147.  [Abstract]

    DEHP (Di-(2-ethylhexyl) phthalate) (100 nM; 3 months) enhanced MM cell viability.

    DEHP purchased from MedChemExpress. Usage Cited in: J Hazard Mater. 2025 Sep 5:495:139147.  [Abstract]

    DEHP (Di-(2-ethylhexyl) phthalate) (100 nM; 3 months) increased the migration and invasion abilities of MM cells.

    DEHP purchased from MedChemExpress. Usage Cited in: J Hazard Mater. 2025 Sep 5:495:139147.  [Abstract]

    DEHP (Di-(2-ethylhexyl) phthalate) (100 nM; 3 months) elevated levels of cyclin D1, CDK2 and P21 in MM cells.

    DEHP purchased from MedChemExpress. Usage Cited in: J Hazard Mater. 2025 Sep 5:495:139147.  [Abstract]

    DEHP (Di-(2-ethylhexyl) phthalate) (100 nM; 3 months) increased the transcriptional levels of MYC, SOX2, OCT3/4, NQO1, GCLC, GSTM2, TXNRD1, and HMOX1 in MM cells.

    DEHP purchased from MedChemExpress. Usage Cited in: J Hazard Mater. 2025 Sep 5:495:139147.  [Abstract]

    DEHP (Di-(2-ethylhexyl) phthalate) (100 nM; 3 months) led to an increase in both tumor weight and volume in the RPMI-8226-derived and U266-derived xenograft mice.

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    Human Endogenous Metabolite Microbial Metabolite

    • Biological Activity

    • Purity & Documentation

    • References

    • Customer Review

    Description

    DEHP (Bis(2-ethylhexyl) phthalate) is a widely used plasticizer, which has orally active. DEHP can produce a wide spectrum of toxic effects on organisms including neurotoxicity, liver toxicity, immunotoxicity, and reproductive toxicity[1][2].

    Cellular Effect
    Cell Line Type Value Description References
    NCI-H460 IC50
    > 50 μM
    Compound: 14
    Cytotoxicity against human NCI-H460 cells incubated for 24 hrs by MTT assay
    Cytotoxicity against human NCI-H460 cells incubated for 24 hrs by MTT assay
    		[PMID: 28606759]
    In Vitro

    DEHP (0-160 µM, 48 h) dramatically inhibits cell viability in mouse GC-1 spg cells[1].
    DEHP (0-160 µM, 48 h) induces autophagy and apoptosis of mouse GC-1 spg cells via oxidative stress[1].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    DEHP Related Antibodies

    Western Blot Analysis[1]

    Cell Line: Mouse GC-1 spg cells
    Concentration: 0-160 µM
    Incubation Time: 48 h
    Result: Decreased Bcl-2 level.
    Increased the levels of Bax, cleaved Caspase-3, and cleaved Caspase-8.
    Increased the levels of LC3-II, Beclin1, and Atg5, as well as the ratio of LC3-II/LC3-I.
    In Vivo

    DEHP (0-500 mg/kg, p.o., daily, 8 weeks) impairs fertility of female C3H/N mice[2].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Animal Model: Female C3H/N mice[1]
    Dosage: 0-500 mg/kg
    Administration: p.o., daily, 8 weeks
    Result: Increased the body weight and visceral fat tissue.
    Increased the expression of PPARα and PPARγ mRNA at the dose of 500 mg/kg.
    Decreased mRNA expression of PPARα transcripts/106 18S molecules at the dose of 500 mg/kg.
    Reduced the level of adiponectin mRNA.
    Increased the mRNA concentration of FABP4 at the dose of 0.05 mg/kg.
    Molecular Weight

    390.56

    Formula

    C24H38O4
    CAS No.
    Appearance

    Liquid (Density: 0.985 g/cm3)

    Color

    Colorless to light yellow

    SMILES

    O=C(C1=CC=CC=C1C(OCC(CC)CCCC)=O)OCC(CC)CCCC
    Structure Classification
    Initial Source
    Shipping

    Room temperature in continental US; may vary elsewhere.
    Storage

    4°C, protect from light

    *In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

    Solvent & Solubility
    In Vitro: 

    DMSO : 150 mg/mL (384.06 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

    0.1 M NaOH : 6.67 mg/mL (17.08 mM; ultrasonic and warming and adjust pH to 10 with 0.1 M NaOH and heat to 60°C)

    H2O : < 0.1 mg/mL (insoluble)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 2.5604 mL 12.8021 mL 25.6043 mL
    5 mM 0.5121 mL 2.5604 mL 5.1209 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    • Molarity Calculator

    • Dilution Calculator

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    Mass
    =
    Concentration
    ×
    Volume
    ×
    Molecular Weight *

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    Concentration (start)

    C1

    ×
    Volume (start)

    V1

    =
    Concentration (final)

    C2

    ×
    Volume (final)

    V2

    In Vivo:

    Select the appropriate dissolution method based on your experimental animal and administration route.

    For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
    To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

      Solubility: ≥ 1.25 mg/mL (3.20 mM); Clear solution

      This protocol yields a clear solution of ≥ 1.25 mg/mL (saturation unknown).

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (12.5 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

      Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
    • Protocol 2

      Add each solvent one by one:  10% DMSO    90% Corn Oil

      Solubility: ≥ 1.25 mg/mL (3.20 mM); Clear solution

      This protocol yields a clear solution of ≥ 1.25 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (12.5 mg/mL) to 900 μL Corn oil, and mix evenly.

    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

    Dosage

    mg/kg

    Animal weight
    (per animal)

    g

    Dosing volume
    (per animal)

    μL

    Number of animals

    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Please enter your animal formula composition:
    %
    DMSO +
    % 
    +
    %
    Tween-80 +
    %
    Saline
    Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
    The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
    Calculation results:
    Working solution concentration: mg/mL
    Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).

    *In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

    The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
    Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
     If the continuous dosing period exceeds half a month, please choose this protocol carefully.
    Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
    Purity & Documentation
    References

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    0.1 M NaOH / DMSO 1 mM 2.5604 mL 12.8021 mL 25.6043 mL 64.0107 mL
    5 mM 0.5121 mL 2.5604 mL 5.1209 mL 12.8021 mL
    10 mM 0.2560 mL 1.2802 mL 2.5604 mL 6.4011 mL
    15 mM 0.1707 mL 0.8535 mL 1.7070 mL 4.2674 mL
    DMSO 20 mM 0.1280 mL 0.6401 mL 1.2802 mL 3.2005 mL
    25 mM 0.1024 mL 0.5121 mL 1.0242 mL 2.5604 mL
    30 mM 0.0853 mL 0.4267 mL 0.8535 mL 2.1337 mL
    40 mM 0.0640 mL 0.3201 mL 0.6401 mL 1.6003 mL
    50 mM 0.0512 mL 0.2560 mL 0.5121 mL 1.2802 mL
    60 mM 0.0427 mL 0.2134 mL 0.4267 mL 1.0668 mL
    80 mM 0.0320 mL 0.1600 mL 0.3201 mL 0.8001 mL
    100 mM 0.0256 mL 0.1280 mL 0.2560 mL 0.6401 mL
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    DEHP Related Classifications

    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    DEHP117-81-7Bis(2-ethylhexyl) phthalate Ergoplast FDO ESBO-D 82Environmental PollutantsEndogenous MetaboliteEnvironmental Contaminantsplasticizerorally activeneurotoxicityliver toxicityimmunotoxicityreproductive toxicityInhibitorinhibitorinhibit

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