DEHP
Based on 13 publication(s) in Google Scholar
DEHP (Bis(2-ethylhexyl) phthalate) is a widely used plasticizer, which has orally active. DEHP can produce a wide spectrum of toxic effects on organisms including neurotoxicity, liver toxicity, immunotoxicity, and reproductive toxicity.
For research use only. We do not sell to patients.
- Purity: 99.56%
- CAS No.: 117-81-7
- Formula: C24H38O4
- Molecular Weight:390.56
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Storage:
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
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Publications Citing Use of MedChemExpress (MCE) DEHP
More- J Hazard Mater. 2025 Sep 5:495:139147. [Abstract]
- Environ Int. 2026 Apr:210:110254. [Abstract]
- Environ Int. 2024 Jun 24:190:108851. [Abstract]
- Int J Biol Macromol. 2026 May:360:151847. [Abstract]
- Ecotoxicol Environ Saf. 2025 Sep 1:302:118689. [Abstract]
- J Environ Sci. 2025 Sep 20.
- Chem Biol Interact. 2025 Sep 5:111738. [Abstract]
- Chem Biol Interact. 2025 Sep 26:111758. [Abstract]
- Poult Sci. 2025 Jun;104(6):105074. [Abstract]
- Poult Sci. 2025 Jan;104(1):104558. [Abstract]
- Toxicol Appl Pharmacol. 2026 Jul:512:117858. [Abstract]
- Toxicol Appl Pharmacol. 2026 Jan:506:117655. [Abstract]
- Sci Total Environ. 2022 Oct 10:842:156815. [Abstract]
Customer Validation & Images
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Cell Proliferation/Viability Assay
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Cell Migration/Invasion Assay
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WB
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RT-PCR
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In Vivo Efficacy Study
All Endogenous Metabolite Isoforms
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Biological Activity
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Cell Line
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Type | Value | Description | References |
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| NCI-H460 | IC50 |
>50 μM
Compound: 14
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Cytotoxicity against human NCI-H460 cells incubated for 24 hrs by MTT assay
Cytotoxicity against human NCI-H460 cells incubated for 24 hrs by MTT assay
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[PMID: 28606759] |
DEHP (0-160 µM, 48 h) dramatically inhibits cell viability in mouse GC-1 spg cells[1].
DEHP (0-160 µM, 48 h) induces autophagy and apoptosis of mouse GC-1 spg cells via oxidative stress[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:Mouse GC-1 spg cells
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Concentration:0-160 µM
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Incubation Time:48 h
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Result:Decreased Bcl-2 level.
Increased the levels of Bax, cleaved Caspase-3, and cleaved Caspase-8.
Increased the levels of LC3-II, Beclin1, and Atg5, as well as the ratio of LC3-II/LC3-I.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:Female C3H/N mice[1]
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Dosage:0-500 mg/kg
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Administration:p.o., daily, 8 weeks
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Result:Increased the body weight and visceral fat tissue.
Increased the expression of PPARα and PPARγ mRNA at the dose of 500 mg/kg.
Decreased mRNA expression of PPARα transcripts/106 18S molecules at the dose of 500 mg/kg.
Reduced the level of adiponectin mRNA.
Increased the mRNA concentration of FABP4 at the dose of 0.05 mg/kg.
Chemical Information
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CAS No. 117-81-7
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Appearance Liquid (Density: 0.985 g/cm3)
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Molecular Weight 390.56
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Formula C24H38O4
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Color Colorless to light yellow
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SMILES
O=C(C1=CC=CC=C1C(OCC(CC)CCCC)=O)OCC(CC)CCCC
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Synonyms
Bis(2-ethylhexyl) phthalate; Ergoplast FDO; ESBO-D 82
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Structure Classification
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Initial Source
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
Publications (13)
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Journal Impact Factor
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Most Recent
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J Hazard Mater
Di-(2-ethylhexyl) phthalate (DEHP) and its metabolite promote multiple myeloma progression and bortezomib resistance. [Abstract]2025 Sep 5:495:139147. PMID: 40633344
DEHP purchased from MedChemExpress. Usage Cited in: J Hazard Mater. 2025 Sep 5:495:139147. [Abstract]
DEHP (Di-(2-ethylhexyl) phthalate) (100 nM; 3 months) enhanced MM cell viability.
DEHP purchased from MedChemExpress. Usage Cited in: J Hazard Mater. 2025 Sep 5:495:139147. [Abstract]
DEHP (Di-(2-ethylhexyl) phthalate) (100 nM; 3 months) increased the migration and invasion abilities of MM cells.
DEHP purchased from MedChemExpress. Usage Cited in: J Hazard Mater. 2025 Sep 5:495:139147. [Abstract]
DEHP (Di-(2-ethylhexyl) phthalate) (100 nM; 3 months) elevated levels of cyclin D1, CDK2 and P21 in MM cells.
DEHP purchased from MedChemExpress. Usage Cited in: J Hazard Mater. 2025 Sep 5:495:139147. [Abstract]
DEHP (Di-(2-ethylhexyl) phthalate) (100 nM; 3 months) increased the transcriptional levels of MYC, SOX2, OCT3/4, NQO1, GCLC, GSTM2, TXNRD1, and HMOX1 in MM cells.
DEHP purchased from MedChemExpress. Usage Cited in: J Hazard Mater. 2025 Sep 5:495:139147. [Abstract]
DEHP (Di-(2-ethylhexyl) phthalate) (100 nM; 3 months) led to an increase in both tumor weight and volume in the RPMI-8226-derived and U266-derived xenograft mice.
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Environ Int
From phthalate exposure to venous thrombotic risk: Decoding the DEHP-ROS mediated OTUD1- p53 stabilization loop in endothelium. [Abstract]2026 Apr:210:110254. PMID: 41967174 -
Environ Int
Effects of BBIBP-CorV vaccine on gut microbiota and short-chain fatty acids in mice exposed to bis (2-ethylhexyl) phthalate and dioctyl terephthalate. [Abstract]2024 Jun 24:190:108851. PMID: 38941942 -
Int J Biol Macromol
2026 May:360:151847. PMID: 41937013 -
Ecotoxicol Environ Saf
Mechanistic insight into plasticizer di-(2-ethylhexyl) phthalate as an environmental hazard for abdominal aortic aneurysm: Evidence from in vitro and in vivo studies. [Abstract]2025 Sep 1:302:118689. PMID: 40680446 -
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Chem Biol Interact
Involvement of ductal reaction in Di-(2-ethylhexyl)-phthalate-caused hepatic fibrosis: Molecular mechanisms and potential intervention strategies. [Abstract]2025 Sep 5:111738. PMID: 40915524 -
Chem Biol Interact
Mechanisms and targeted prevention of chronic pancreatitis-acinar to ductal metaplasia caused by a low concentration of di-(2-ethylhexyl)-phthalate. [Abstract]2025 Sep 26:111758. PMID: 41016453 -
Poult Sci
New insights into DEHP-induced inflammatory injury in chicken spleen: ROS/TLR4/MyD88 pathway and apoptosis/necroptosis-M1 polarization crosstalk. [Abstract]2025 Jun;104(6):105074. PMID: 40245538 -
Poult Sci
ROS-mediated M1 polarization-necroptosis crosstalk involved in Di-(2-ethylhexyl) phthalate-induced chicken liver injury. [Abstract]2025 Jan;104(1):104558. PMID: 39631278 -
Toxicol Appl Pharmacol
Shared inflammatory targets of phthalate plasticizers in MODS-related syndromes: An integrative network toxicology study with representative DEHP in vitro validation. [Abstract]2026 Jul:512:117858. PMID: 42107545 -
Toxicol Appl Pharmacol
Capsaicin alleviates DEHP-induced testicular dysfunction by suppressing oxidative stress in mice. [Abstract]2026 Jan:506:117655. PMID: 41276025 -
Sci Total Environ
Di-(2-ethylhexyl) phthalate increases plasma glucose and induces lipid metabolic disorders via FoxO1 in adult mice. [Abstract]2022 Oct 10:842:156815. PMID: 35750186
Solvent & Solubility
DMSO : 150 mg/mL (384.06 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
0.1 M NaOH : 6.67 mg/mL (17.08 mM; ultrasonic and warming and adjust pH to 10 with 0.1 M NaOH and heat to 60°C)
H2O : < 0.1 mg/mL (insoluble)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 1.25 mg/mL (3.20 mM); Clear solution
This protocol yields a clear solution of ≥ 1.25 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (12.5 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL. * In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
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Data Sheet (276 KB)
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SDS (622 KB)
- English - EN (622 KB)
- Français - FR (622 KB)
- Deutsch - DE (622 KB)
- Norwegian - NO (622 KB)
- Español - ES (622 KB)
- Swedish - SV (622 KB)
- Italian - IT (622 KB)
- Korean - KR (622 KB)
- Portuguese - PT (622 KB)
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Handling Instructions (2659 KB)
References
[1]. Gan Y, et al. Di-2-ethylhexyl phthalate (DEHP) induces apoptosis and autophagy of mouse GC-1 spg cells. Environ Toxicol. 2020 Feb;35(2):292-299. [Content Brief]
[2]. Schmidt JS, et al. Effects of di(2-ethylhexyl) phthalate (DEHP) on female fertility and adipogenesis in C3H/N mice. Environ Health Perspect. 2012 Aug;120(8):1123-9. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| 0.1 M NaOH / DMSO | 1 mM | 2.5604 mL | 12.8021 mL | 25.6043 mL | 64.0107 mL |
| 5 mM | 0.5121 mL | 2.5604 mL | 5.1209 mL | 12.8021 mL | |
| 10 mM | 0.2560 mL | 1.2802 mL | 2.5604 mL | 6.4011 mL | |
| 15 mM | 0.1707 mL | 0.8535 mL | 1.7070 mL | 4.2674 mL | |
| DMSO | 20 mM | 0.1280 mL | 0.6401 mL | 1.2802 mL | 3.2005 mL |
| 25 mM | 0.1024 mL | 0.5121 mL | 1.0242 mL | 2.5604 mL | |
| 30 mM | 0.0853 mL | 0.4267 mL | 0.8535 mL | 2.1337 mL | |
| 40 mM | 0.0640 mL | 0.3201 mL | 0.6401 mL | 1.6003 mL | |
| 50 mM | 0.0512 mL | 0.2560 mL | 0.5121 mL | 1.2802 mL | |
| 60 mM | 0.0427 mL | 0.2134 mL | 0.4267 mL | 1.0668 mL | |
| 80 mM | 0.0320 mL | 0.1600 mL | 0.3201 mL | 0.8001 mL | |
| 100 mM | 0.0256 mL | 0.1280 mL | 0.2560 mL | 0.6401 mL |
DEHP Related Classifications
HY-B1945 Related Classifications
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