Cat. No.: HY-N0761

Isoferulic acid

(Synonyms: 3-Hydroxy-4-methoxycinnamic acid)

3 Cited Publications

Customer Review

Based on 3 publication(s) in Google Scholar

Isoferulic acid (3-Hydroxy-4-methoxycinnamic acid) is an orally active cinnamic acid derivative. Isoferulic acid exhibits hypoglycemic, antiviral, and antioxidant activities. Isoferulic acid can also inhibit fructose- and glucose-mediated protein glycation. Isoferulic acid can be used in the research of diseases such as diabetes.

For research use only. We do not sell to patients.

Purity: 99.91%
CAS No.: 537-73-5
Formula: C₁₀H₁₀O₄
Molecular Weight:194.19

Storage:

Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 2 years , -20°C, 1 year

Isoferulic acid Chemical Structure

CAS No.: 537-73-5

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Top Publications Citing

Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- Cellular Effect
- In Vitro
- In Vivo
Chemical Information
Publications (3)
Solvent & Solubility
- In Vitro
- In Vivo
Purity & Documentation
References
Help & FAQs
Classifications
Bioz

Biological Activity

Description

All Adrenergic Receptor Isoforms :

Cellular Effect

Cell Line	Type	Value	Description	References
A549	EC₅₀	>515 μM Compound: 10	Antiproliferative activity against human A549 cells after 72 hrs by MTT assay Antiproliferative activity against human A549 cells after 72 hrs by MTT assay	[PMID: 12027739]
B16-BL6	EC₅₀	>515 μM Compound: 10	Antiproliferative activity against mouse B16-BL6 cells after 72 hrs by MTT assay Antiproliferative activity against mouse B16-BL6 cells after 72 hrs by MTT assay	[PMID: 12027739]
EA.hy 926	EC₅₀	29.3 μM Compound: 3-OH-4-OCH3-cinnamic acid	Cytoprotective activity against H2O2-induced cytotoxicity in human EAhy926 cells pre-incubated for 24 hrs before H2O2 challenge for 12 hrs by MTT assay Cytoprotective activity against H2O2-induced cytotoxicity in human EAhy926 cells pre-incubated for 24 hrs before H2O2 challenge for 12 hrs by MTT assay	10.1039/C4MD00022F
HeLa	EC₅₀	>515 μM Compound: 10	Antiproliferative activity against human HeLa cells after 72 hrs by MTT assay Antiproliferative activity against human HeLa cells after 72 hrs by MTT assay	[PMID: 12027739]
HeLa	IC₅₀	>=10 μM Compound: isoferulic acid	Cytotoxicity against human HeLa cells by MTT assay Cytotoxicity against human HeLa cells by MTT assay	[PMID: 19278239]
HT-1080	EC₅₀	>515 μM Compound: 10	Antiproliferative activity against human HT1080 cells after 72 hrs by MTT assay Antiproliferative activity against human HT1080 cells after 72 hrs by MTT assay	[PMID: 12027739]
Oocyte	IC₅₀	13.5 μM Compound: 4-O-Me-Caf-COOH	Antagonist activity at Gloeobacter violaceus ligand-gated ion channel expressed in Xenopus oocytes assessed as inhibition of MES buffer pH 5.5 -induced currents after 30 secs by voltage clamp technique Antagonist activity at Gloeobacter violaceus ligand-gated ion channel expressed in Xenopus oocytes assessed as inhibition of MES buffer pH 5.5 -induced currents after 30 secs by voltage clamp technique	[PMID: 23682762]
RAW264.7	IC₅₀	>50 μM Compound: 12	Antiinflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-induced NO production after 24 hrs by Griess method Antiinflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-induced NO production after 24 hrs by Griess method	[PMID: 22079762]

In Vitro

Isoferulic acid has IC₅₀ values of 7.30, 4.58, 1.08, 8.84, 7.69, 1.57 and 13.33 μg/mL, respectively, for lipid peroxidation, DPPH and ABTS radical scavenging, reducing power on Fe³⁺ and Cu²⁺ ions, and hydroxyl and superoxide anion radical scavenging^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

Isoferulic acid (0.5-10 mg/kg; intravenous injection; single dose) has an improving effect in a diabetic rat model^[2].
Isoferulic acid (0.25-1.0 mg/kg; oral administration; 4 days) has antiviral activity in mice infected with the influenza virus^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:

Male Wistar rats weighing 200 to 250 g treated Streptozotocin (HY-13753) ^[2]
Dosage:

0.5, 1, 3, 5 and 10 mg/kg
Administration:

Intravenous injection; single dose
Result:

Dose-dependently lowered plasma glucose concentrations and increased plasma plasma β-endorphin-like immunoreactivity in rats. This effect was abolished by pretreatment with α₁-adrenoceptor antagonists or opioid μ-receptor antagonists.

Animal Model:

ICR female aged 4 weeks old mice (body weight, approximately 17 g) treated influenza virus^[3]
Dosage:

0.25, 0.5 and 1.0 mg/kg
Administration:

Oral administration; 4 days
Result:

Significantly improved survival rate and reduced body weight loss compared to the control group at dose of 0.5 mg/kg.
Had a slightly beneficial effect on survival rates at dose of 0.25 mg/kg, and had no effect at dose of 1 mg/kg.

Chemical Information

CAS No. 537-73-5
Appearance Solid
Molecular Weight 194.19
Formula C₁₀H₁₀O₄
Color White to off-white
SMILES

O=C(O)/C=C/C1=CC=C(OC)C(O)=C1
Synonyms

3-Hydroxy-4-methoxycinnamic acid
Structure Classification
- Phenylpropanoids
- Simple Phenylpropanols
- Phenols
- Monophenols
Initial Source
Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	2 years
	-20°C	1 year

Publications (3)

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Journal Impact Factor

Chin Med

Therapeutic potential of Sheng-Xian-Tang in doxorubicin-induced chronic heart failure by regulation of phenylalanine metabolism disruption. [Abstract]

2026 Jan 12;21(1):29. PMID: 41527105
Vet Microbiol

The Chinese medicine monomer Schisandrin C inhibits PRRSV infection by regulating the OGT-PI3K/AKT/mTOR signaling pathway. [Abstract]

2026 May:316:110992. PMID: 41865607
Iran J Pharm Res

Geranyl Acetate Attenuates Para-phenylenediamine-induced Cytotoxicity, DNA Damage, Apoptosis, and Inflammation in HaCaT Keratinocytes. [Abstract]

2025 Oct 1;24(1):e164379. PMID: 41104235

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (514.96 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

5.1496 mL

25.7480 mL

51.4960 mL

5 mM

1.0299 mL

5.1496 mL

10.2992 mL

10 mM

0.5150 mL

2.5748 mL

5.1496 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Molarity Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Concentration Concentration

Volume Volume

Molecular Weight Molecular Weight

Dilution Calculator

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

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In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline

Solubility: ≥ 2.08 mg/mL (10.71 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)

Solubility: ≥ 2.08 mg/mL (10.71 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil

Solubility: ≥ 2.08 mg/mL (10.71 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

%
DMSO +

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
%
+
+
%
Tween-80 + +
%
Saline +

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Working solution concentration: 0.22 mg/mL

Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (277 KB)
SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Wang X, et al. Evaluation of antioxidant activity of isoferulic acid in vitro. Nat Prod Commun. 2011 Sep;6(9):1285-8. [Content Brief]

[2]. Liu IM, et al. Mediation of beta-endorphin by isoferulic acid to lower plasma glucose in streptozotocin-induced diabetic rats. J Pharmacol Exp Ther. 2003 Dec;307(3):1196-204. [Content Brief]

[3]. Sakai S, et al. Administration of isoferulic acid improved the survival rate of lethal influenza virus pneumonia in mice. Mediators Inflamm. 2001 Apr;10(2):93-6. [Content Brief]

[4]. Meeprom A, et al. Isoferulic acid, a new anti-glycation agent, inhibits fructose- and glucose-mediated protein glycation in vitro. Molecules. 2013 May 30;18(6):6439-54. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	5.1496 mL	25.7480 mL	51.4960 mL	128.7399 mL
	5 mM	1.0299 mL	5.1496 mL	10.2992 mL	25.7480 mL
	10 mM	0.5150 mL	2.5748 mL	5.1496 mL	12.8740 mL
	15 mM	0.3433 mL	1.7165 mL	3.4331 mL	8.5827 mL
	20 mM	0.2575 mL	1.2874 mL	2.5748 mL	6.4370 mL
	25 mM	0.2060 mL	1.0299 mL	2.0598 mL	5.1496 mL
	30 mM	0.1717 mL	0.8583 mL	1.7165 mL	4.2913 mL
	40 mM	0.1287 mL	0.6437 mL	1.2874 mL	3.2185 mL
	50 mM	0.1030 mL	0.5150 mL	1.0299 mL	2.5748 mL
	60 mM	0.0858 mL	0.4291 mL	0.8583 mL	2.1457 mL
	80 mM	0.0644 mL	0.3218 mL	0.6437 mL	1.6092 mL
	100 mM	0.0515 mL	0.2575 mL	0.5150 mL	1.2874 mL

Keywords

Isoferulic acid

3 Cited Publications

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Isoferulic acid

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Publications Citing Use of MedChemExpress (MCE) Isoferulic acid

All Adrenergic Receptor Isoforms

Biological Activity

Chemical Information

Publications (3)

Solvent & Solubility

Purity & Documentation

References

Help & FAQs

Complete Stock Solution Preparation Table

Isoferulic acid Related Classifications

HY-N0761 Related Classifications